Cored anion-exchange chromatography media for antibody flow-through purification

Modeling of Liquid Chromatography with Cored Beads

“…They suffer from flow distortion, resulting in deterioration of separation outcome. Because cored beads have a rigid solid cores that maintain their shape under pressure better, this results in column pressure drops several times lower than a column packed with fully porous beads [3]. This means the columns packed with cored beads have more room for axial direction scale-up.…”

“…9.11 have the same peak area, leading to the same sample volume of 6.36 ml. Because cored beads exhibit bed pressure drops that are several times lower than fully porous beads with the same column dimensions [3], it is possible to scale up a column packed with cored beads by doubling the column length while using a flow rate that is twice as large. Figure 9.13 shows the comparison of ovalbumin separation from myoglobin using fully porous beds (dashed lines) and core beads (solid lines).…”

“…Cored beads are also known as pellicular beads [3][4][5][6] and superficially porous beads [7] with an inert impenetrable solid core to block fluids. A heavy silica or stainless steel core may be used to achieve this objective.…”

“…These beads have desirable hydrodynamic performances such as reduced nonideal flow and pressure drop [9,10]. They have been used in various LC separations including ion exchange [3,11,12]. Some commercially available cored beads were reviewed and compared experimentally by Cabooter et al [13].…”

Application of a PEG/salt aqueous two‐phase partition system for the recovery of monoclonal antibodies from unclarified transgenic tobacco extract

“…These include cation exchange chromatography [16], anion exchange chromatography [17] Protein isolation and purification from plants is a difficult task, owing to the complexity of the plant system and the presence of phenolic compounds, pigments, and mucilages [14,31]. The presence of such compounds is potential interfering factor in downstream processing and has to be addressed early in the isolation process.…”