Core–shell patterning of synthetic hydrogels <i>via</i> interfacial bioorthogonal chemistry for spatial control of stem cell behavior

Dicker, Kevin T.; Song, Jianzheng; Moore, Axel C.; Zhang, Han; Li, Y.; Burris, David L.; Jia, Xinqiao; Fox, Joseph M.

doi:10.1039/c8sc00495a

Cited by 32 publications

(53 citation statements)

References 80 publications

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“…[ 166 ] Previously, flat substrate‐based 2D cell models enable the automated and high throughput cell assay performance in vitro, which cannot provide useful tools to analyze cell behaviors in spatial cell microenvironments. Recently, in synthetic ECM, some new techniques are described for the patterning of cell behaviors guidance cues in 3D cell models, [ 167 ] where cell differentiation or cell behaviors are controlled by the synthetic hydrogels with the defined guidance cues in a biomimetic fashion. It is a helpful bioengineering methodology to customize the synthetic hydrogels to develop 3D cell models.…”

Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Yang

Zhao

2020

Advanced Science

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mouse intestinal stem cells and primary colorectal cancer cells can be propagated in vitro long term, [1] there is an urgent need to develop more physiologically relevant, efficient, and robust precise oncology models that closely recapitulate the genetic and morphological heterogeneous composition and mimic the arrangement pattern of cancer cells in the original tumor. [2] To our knowledge in biomedical research, hydrogel is the best tool to reconstruct precise oncology models in vitro, especially tumor organoid, which not only recapitulates in vivo biology and microenvironmental factors, but also at large extent allows side-by-side comparison to evaluate the translational potential of 3D model systems to the patients. [2a,3] Generally, hydrogels are mainly composed of hydrophilic polymeric scaffolds that absorb large amounts of water, so the hydrogel matrix better mimics elastic and viscoelastic properties in ECM and microscale topographies of cell matrices, which controls proper cell morphology, directs viable cell behaviors, and drives in vivo fundamental cell-cell or cell-ECM interactions. [4] To recapitulate pathophysiological features of human tumors and imitate various aspects of human tumorigenesis in vivo, hydrogels can provide a realistic platform to establish a useful bridge between in vitro assays and in vivo cell microenvironments. In current biomedical applications, there are many kinds of hydrogels to be developed to mimic the biological properties of ECM, such Designer self-assembling peptides form the entangled nanofiber networks in hydrogels by ionic-complementary self-assembly. This type of hydrogel has realistic biological and physiochemical properties to serve as biomimetic extracellular matrix (ECM) for biomedical applications. The advantages and benefits are distinct from natural hydrogels and other synthetic or semisynthetic hydrogels. Designer peptides provide diverse alternatives of main building blocks to form various functional nanostructures. The entangled nanofiber networks permit essential compositional complexity and heterogeneity of engineering cell microenvironments in comparison with other hydrogels, which may reconstruct the tumor microenvironments (TMEs) in 3D cell cultures and tissue-specific modeling in vitro. Either ovarian cancer progression or recurrence and relapse are involved in the multifaceted TMEs in addition to mesothelial cells, fibroblasts, endothelial cells, pericytes, immune cells, adipocytes, and the ECM. Based on the progress in common hydrogel products, this work focuses on the diverse designer self-assembling peptide hydrogels for instructive cell constructs in tissue-specific modeling and the precise oncology remodeling for ovarian cancer, which are issued by several research aspects in a 3D context. The advantages and significance of designer peptide hydrogels are discussed, and some common approaches and coming challenges are also addressed in current complex tumor diseases.

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Section: Instructive Cell Constructs In Tissue Engineering and Precismentioning

confidence: 99%

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Yang

Zhao

2020

Advanced Science

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“…In this study, the unique semisynthetic protein‐PEG hydrogel matrices yielded tunable stiffness‐patternable features which could be cycled on demand to investigate 3D cellular response to cyclic loading, a key aspect that native ECM is subjected to but was yet to be replicated in cell‐laden hydrogel constructs (Figure G). In another sophisticated work, researchers have manufactured core–shell hyaluronic‐based hydrogels with distinct biochemical and biophysical features . Cell adhesion site density, enzymatic degradability, and mechanical stiffness could be hierarchically presented in a core–shell bioarchitecture by varying the macromer/crosslinker ratios and timing of introduction of the interfacial crosslinking agents that covalently functionalized hydrogels at the liquid‐gel interface.…”

Section: Cell–biomaterials Assembliesmentioning

confidence: 99%

“…Another relevant strategy to obtain cellularly graded hydrogels exploits the self‐healing phenomena that is characteristic of dynamic covalent hydrogel networks. Using this approach cell‐laden hydrogels based on 2‐acrylamidophenylboronic acid and poly(vinyl alcohol), which readily self‐assembled in aqueous conditions were successfully fabricated . Due to the dynamic nature of boronic‐diol bonds, these hydrogels exhibited significant self‐healing capacity, which was exploited for establishing a gradient of two different cell types (e.g., lung fibroblasts and breast cancer cells) initially cultured separately on different hydrogels.…”

Section: Cell–biomaterials Assembliesmentioning

confidence: 99%

Advanced Bottom‐Up Engineering of Living Architectures

et al. 2019

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Bottom‐up tissue engineering is a promising approach for designing modular biomimetic structures that aim to recapitulate the intricate hierarchy and biofunctionality of native human tissues. In recent years, this field has seen exciting progress driven by an increasing knowledge of biological systems and their rational deconstruction into key core components. Relevant advances in the bottom‐up assembly of unitary living blocks toward the creation of higher order bioarchitectures based on multicellular‐rich structures or multicomponent cell–biomaterial synergies are described. An up‐to‐date critical overview of long‐term existing and rapidly emerging technologies for integrative bottom‐up tissue engineering is provided, including discussion of their practical challenges and required advances. It is envisioned that a combination of cell–biomaterial constructs with bioadaptable features and biospecific 3D designs will contribute to the development of more robust and functional humanized tissues for therapies and disease models, as well as tools for fundamental biological studies.

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“…Recent reviews summarizing advances in cross-linking chemistries are available; [35,36] these are focused on commonly used PEG-based hydrogels. [37][38][39], PLA-b-PEG-b-PLA [4], PVA [4], chondroitin sulfate [4], alginate [40], hyaluronic acid [41][42][43], collagen [44,45], chitosan [46,47], gelatin [48][49][50] Aortic smooth muscle cells [37,44], calvaria osteoblasts [38], pancreactic [39], articular chondrocytes [40,46,47,51,52] [78,79], hyaluronic acid [79][80][81] Mesenchymal stem cells [78,81], prostate cancer cells [80], bone marrow mesenchymal stem cells [79] Native chemical ligation Ethyl 3-mercaptoproprionate, Boc-Cys(Trt)-OH PEG [82][83][84] Insulinoma cells [83], mesenchymal stem cells [84], induced pluripotent stem cells [84] Imine formation Sodium periodate (oxidative cleavage of vicinal diols), 4-formylbenzoic acid, ethylene diamine…”

Section: Cell Encapsulation: Implications For Hydrogel Cross-linking mentioning

confidence: 99%

Chemical cross-linking methods for cell encapsulation in hydrogels

Echalier

Valot

Martínez

et al. 2019

Materials Today Communications

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Cell-encapsulating hydrogels are of tremendous interest in regenerative medicine. Tissue engineering relies on biomaterials able to act as artificial extracellular matrices to guide cells towards the development of new tissues. Therefore, considerable efforts have been made to design biomaterials which mimic cells' native environment, thus encouraging natural behavior. The choice of biomaterial in which cells are embedded is crucial for their survival, proliferation and differentiation. Being more stable, chemical hydrogels are preferred over physical hydrogels as cell-laden substrates. When designing chemical hydrogels, scientists must choose not only the nature of the network (synthetic and/or biopolymers) but also the type of cross-link bridging hydrogel constituents. For that purpose, numerous chemistries have been used (i) to introduce reactive functions on the hydrogel precursors and (ii) to form covalent bonds in the presence of living cells. The review will discuss the advantages and limitations of each strategy.

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Core–shell patterning of synthetic hydrogels via interfacial bioorthogonal chemistry for spatial control of stem cell behavior

Abstract: A new technique is described for the patterning of cell-guidance cues in synthetic extracellular matrices.

Cited by 32 publications

References 80 publications

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Designer Self‐Assembling Peptide Hydrogels to Engineer 3D Cell Microenvironments for Cell Constructs Formation and Precise Oncology Remodeling in Ovarian Cancer

Advanced Bottom‐Up Engineering of Living Architectures

Chemical cross-linking methods for cell encapsulation in hydrogels

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