Core–shell molecularly imprinted polymer nanoparticles with assistant recognition polymer chains for effective recognition and enrichment of natural low-abundance protein

Liu, Dejing; Yang, Qian; Jin, Susu; Song, Yingying; Gao, Junfei; Wang, Ying; Mi, Huaifeng

doi:10.1016/j.actbio.2013.10.007

Cited by 44 publications

(24 citation statements)

References 34 publications

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“…Surface imprinting is an emerging technique that creates sites on the surface of suitable support materials. The sites are complementary with template molecules in shape, size and functional groups and favor rebinding of template molecules to other compounds with similar structures . Generally, surface molecularly imprinted polymers possess many advantages, for instance, easier extraction of the template molecule, better mechanical stability, larger surface area and faster mass transfer than traditional molecularly imprinted polymers (MIPs).…”

Section: Introductionmentioning

confidence: 99%

Bovine serum albumin surface imprinted polymer fabricated by surface grafting copolymerization on zinc oxide rods and its application for protein recognition

Zhou

Tian

et al. 2015

J. Sep. Science

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A novel bovine serum albumin (BSA) surface imprinted polymer based on ZnO rods was synthesized by surface grafting copolymerization. It exhibited an excellent recognition performance to bovine serum albumin. The adsorption capacity and imprinting factor of bovine serum albumin could reach 89.27 mg/g and 2.35, respectively. Furthermore, the fluorescence property of ZnO was used for tracing the process of protein imprinting and it implied the excellent optical sensing property of this material. More importantly, the hypothesis that the surface charge of carrier could affect the imprinting process was confirmed. That is, ZnO with positive surface charge could not only improve the recognition specificity of binding sites to template proteins (pI < 7), but also deteriorate the bindings between sites and non-template proteins (pI > 7). It was also important that the reusability of ZnO@BSA molecularly imprinted polymers was satisfactory. This implied that the poor mechanical/chemical stability of traditional zinc oxide sensors could be solved by the introduction of surface grafting copolymerization. These results revealed that the ZnO@BSA molecularly imprinted polymers are a promising optical/electrochemical sensor element.

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Section: Introductionmentioning

confidence: 99%

Bovine serum albumin surface imprinted polymer fabricated by surface grafting copolymerization on zinc oxide rods and its application for protein recognition

Zhou

Tian

et al. 2015

J. Sep. Science

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“…The direct assembly between template and functional monomer in the polymeric forming mixture enables the formation of discrete cavities to provide specific interaction with the template when rebinding. 3,4 Thus, this special characteristic of MIP technology has been employed in the identification and purification of protein and high molecular structures such as chromatographic resin, solid-phase adsorbent, bio mimetic sensor, reaction catalyst and enzyme mimetic. [5][6][7][8][9] Before the MIP can be used, it is essential to eliminate the template molecules from the polymer cavities.…”

Section: Introductionmentioning

confidence: 99%

Heparin imprinted polymer prepared by sol–gel process on silica microparticles surface: Analysis of template removal and binding performance

Nasir

Ahmad

Dzahir

et al. 2014

Materials Research Innovations

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A surface molecular imprinted polymer on silica gel particles for highly selective recognition of heparin (sulphated glycosaminogylcan) was prepared by a sol-gel process. The low molecular weight heparin was exploited as the template model in preparation of the non-covalent surface imprinted polymer. Meanwhile the primary amine functioned silane was utilised as the key functional monomer for biologically relevant heparin sulphate sequences-specific recognition. The heparin imprinted silica microparticles were characterised by Fourier transform infrared spectroscopy and scanning electron micrograph. Since there was template bleeding following the first extraction, a different washing solution type and template removal strategies have been developed to overcome the problem that was interfering with the binding performance. The finding of this study shows that the MeOH acid removes the higher amount of template from the imprinting polymer. The best rebinding analyte solution was obtained with acidic medium at both stirring and ultrasonic extraction.

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“…have also been reported. [19][20][21][22][23][24][25][26] Despite the signicant progress, there are still some problems, especially when proteins are applied as template/target. 4 The major problem is the restricted mass transfer across the cross-linked polymer matrix due to the large sizes of proteins, which limits the ease of template removal as well as target rebinding.…”

Section: Introductionmentioning

confidence: 99%

A highly selective protein adsorber via two-step surface-initiated molecular imprinting utilizing a multi-functional polymeric scaffold on a macroporous cellulose membrane

Liu

Ulbricht

2017

RSC Adv.

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Core–shell molecularly imprinted polymer nanoparticles with assistant recognition polymer chains for effective recognition and enrichment of natural low-abundance protein

Cited by 44 publications

References 34 publications

Bovine serum albumin surface imprinted polymer fabricated by surface grafting copolymerization on zinc oxide rods and its application for protein recognition

Bovine serum albumin surface imprinted polymer fabricated by surface grafting copolymerization on zinc oxide rods and its application for protein recognition

Heparin imprinted polymer prepared by sol–gel process on silica microparticles surface: Analysis of template removal and binding performance

A highly selective protein adsorber via two-step surface-initiated molecular imprinting utilizing a multi-functional polymeric scaffold on a macroporous cellulose membrane

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