Core–Shell Heterostructured CuFe@FeFe Prussian Blue Analogue Coupling with Silver Nanoclusters via a One-Step Bioinspired Approach: Efficiently Nonlabeled Aptasensor for Detection of Bleomycin in Various Aqueous Environments

Abstract

We synthesized novel core−shell heterostructured Prussian blue analogue (PBA) nanospheres coupled with silver nanoclusters (AgNCs) via a one-step bioinspired approach and further exploited these as aptasensors for the detection of a trace antibiotic, bleomycin (BLM). Using FeFe Prussian blue (FeFe PB) as the core, a bimetallic CuFe@FeFe PBA layer was prepared by coupling with AgNCs synthesized by taking the BLM-targeted aptamer as a template (denoted by AgNCs/Apt@CuFe@FeFe). The coupling of AgNCs/Apt via a one-step bioinspired approach not only can improve the sensing performance of CuFe@FeFe-based aptasensors but also can shorten the aptasensor fabrication procedure. Due to the strong coordination interaction between abundant Fe(II) ions contained in CuFe@FeFe PBA nanospheres and BLM (represented by Fe(II)•BLM), the Fe(II)•BLM complex formed enables aptamer strands to undergo an irreversible cleavage event that can result in a significant change in electrochemical activity. Electrochemical results displayed

Recent Advances in (Bio)Chemical Sensors for Food Safety and Quality Based on Silver Nanomaterials

“…In these cases, the dried particles were either purchased ( 39 , 40 ), or isolated from the reaction suspension applying centrifugation ( 41 , 42 ), magnet separation ( 43 ), electrodeposition ( 34 , 44 ) or solution drying techniques ( 45 , 46 ). Furthermore, crystalline nanosilver product can also be obtained by implementing precipitation agents ( 35 , 47 ). Recently, we have shown that weak organic acids ( 48 ), as well as strong mineral acids ( 49 ), can be successfully applied to precipitate polyacid-stabilised nanosilver grains as the functional component of printable conductive inks.…”

“…A current trend in the sensor field is directed towards solving analytical problems with the development of cost-effective, miniaturised and portable devices that could be operated in the field ( 54 ). A survey of the silver nanomaterial-based electrochemical sensors for food applications published in the last five years is listed in Table 1 ( 32 , 34 , 35 , 39 , 40 , 44 , 45 , 47 , 55 - 85 ). Selected examples of electrochemical sensors, both voltammetric and impedimetric, are shown in Fig.…”

“…Therefore, the trace antibiotic levels in food products need to be strictly regulated ( 100 ). Combining Prussian blue (FeFe-PB) core with bimetallic CuFe shell, and coupling with silver nanoclusters via aptamer linkage in a one-step bio-inspired synthesis, an impedimetric aptasensor for bleomycin (BLM) antibiotic was developed ( 47 ). Owing to the formation of Fe(II)·BLM complex, the AgNCs/Apt@CuFe@FeFe aptasensor outperformed the CuFe@FeFe-based one, giving an extremely low detection limit of 0.0082 fg/mL towards BLM.…”

Quantification of EGFR and EGFR-overexpressed cancer cells based on carbon dots@bimetallic CuCo Prussian blue analogue

“…29 Core-shell heterostructured PBA nanospheres were composited with silver nanoclusters and further applied as aptasensors for detecting bleomycin. 30 Especially, PBA nanocube precursors containing different metal ion centers such as nickel, cobalt, and iron were used to keep a general nanocuboidal structure while optimizing the composition of metal centers to efficiently anchor the probe biomolecules. Nevertheless, PBA-based biosensors for detecting EGFR have not been reported.…”

A One-Step Dual-Mode Aptasensor for Subnanomolar Detection of Lead Ions Based on Electrochemiluminescence and Fast Scan Voltammetry

“…Therefore, one-step electrochemical biosensors have attracted considerable interest, due to their promising application in the medical diagnostics, food safety fields, and environmental monitoring. [32][33][34] Up to now, one-step aptasensors for Pb 2+ are rarely reported. 35 In order to improve the reliability of detection results, dual-mode detection has attracted much attention in recent years.…”