Core‐shell fibrous scaffold as a vehicle for sustained release of retinal pigmented epithelium‐derived factor (PEDF) for photoreceptor differentiation of conjunctiva mesenchymal stem cells

Abstract

Coaxial electrospinning technique was introduced as a flexible and promising technique for the fabrication of core-shell fibrous scaffold from poly ethylene glycol/poly caprolactone (PEG/PCL), where retinal pigmented epithelium-derived factor (PEDF) was encapsulated in the core, for photoreceptor differentiation of conjunctiva mesenchymal stem cells (CJMSCs) seed on scaffolds. The morphology and structure of fibers were characterized using SEM and TEM and photoreceptor differentiation was examined by quantitative real time PCR (qPCR). Release study showed that, a sustained release of PEDF from PEG/PCL scaffold was observed over 14 days. qPCR analysis demonstrated that rhodopsin (as a main photoreceptor gene) was significantly expressed in CJMSCs cultured on scaffold loaded with PEDF. According to the result, the core-shell scaffold loaded with PEDF (PEG + PEDF)/PCL) has superior control over factor release profile and has a potential for guiding photoreceptor differentiation of mesenchymal stem cells and promoting retinal regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3514-3519, 2017.

Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells

“…In this study, a conductive scaffold consist of SF/rGo was fabricated by electrospinning method to evaluate electrical stimulus on the proliferation and differentiation of CJMSCs toward neural‐like cells. Electrospinning as an electrostatic technique includes the use of a high‐voltage electrostatic field to charge the polymer solution which results in the creation of solidified ultra‐thin fibers as a scaffold for biomedical and tissue engineering application . Nanofibrous scaffold gives an excellent opportunity to the tissue engineering field because of its similarity to native ECM .…”

“…Electrospinning as an electrostatic technique includes the use of a high-voltage electrostatic field to charge the polymer solution which results in the creation of solidified ultra-thin fibers as a scaffold for biomedical and tissue engineering application. 21,22,43 Nanofibrous scaffold gives an excellent opportunity to the tissue engineering field because of its similarity to native ECM. 44 To date, synthetic and natural biomaterials have been used for scaffold fabrication.…”

“…The isolated stromal tissue was incubated in DMEM/F‐12 supplemented with 10% FBS, 10 ng/mL human LIF (Chemicon, Temecula, CA, USA), 4 ng/mL basic‐FGF (Peprotech, Rocky Hill, NJ, USA), and 5 mg/mL insulin (Sigma). To identify the nature of cells, they were induced with osteogenic (DMEM including 10 nM dexamethasone [Sigma], 10 mM b‐glycerophosphate [Sigma], 50 mg/mL ascorbic acid 2‐phosphate [Sigma]), adipogenic (DMEM supplemented with 50 mg/mL indomethacine [Sigma], and 100 nM dexamethasone [Sigma]), chondrogenic (DMEM supplemented with 10 ng/mL transforming growth factor‐ß3 [TGF‐ß3; Sigma], 50 mg/mL ascorbate‐2‐phosphate [Sigma], 10‐7 M dexamethasone [Sigma], bone morphogenetic protein‐6 [BMP‐6], and 50 ug/mL insulin‐transferrin‐selenium [ITS; GIBCO]) medium for 21 days …”

Potential core–shell designed scaffolds with a gelatin‐based shell in achieving controllable release rates of proteins for tissue engineering approaches

“…The process of protein emulsions preparation and the thermodynamic instability of emulsion arises challenges . Hence, coaxial electrospinning is considered as one of the most remarkable breakthroughs in the area of susceptible agents release …”

“…14,21 Hence, coaxial electrospinning is considered as one of the most remarkable breakthroughs in the area of susceptible agents release. [21][22][23][24] Correspondence to: M. Latifi; e-mail: latifi@aut.ac.ir or A. Hadjizadeh; e-mail: afra.hajizadeh@aut.ac.ir Coaxial electrospinning is a modified conventional electrospinning to produce micro/nanofibers with core-shell architecture. 25,26 In this process, at least two fluids are independently fed through a coaxial nozzle comprising inner/outer orifice and drawn to fabricate core-shell structured fibers.…”