Core Components of Abscisic Acid Signaling and Their Post-translational Modification

Lim, Junsub; Lim, Chae Woo; Lee, Sung Chul

doi:10.3389/fpls.2022.895698

Cited by 15 publications

(9 citation statements)

References 146 publications

(196 reference statements)

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“…This indicates that the enhanced seed germination of the bro1-1 mutant may be due to increased ion and osmotic signalling; this signalling can induce downstream stress-responsive genes, such as the genes that respond to ABA, as shown in Figures 10B, C and Supporting Information Tables S2-5. These genes are involved in abiotic stress responses and contribute to plant resistance to various stress factors when overexpressed (Golldack et al, 2014;Lim et al, 2022). This analysis confirms that ABA-regulated genes are involved in AtBro1-mediated abiotic stress tolerance.…”

Section: Discussionsupporting

confidence: 68%

“…The core ABA signaling pathway is also vital for the regulation of plant growth and development In Arabidopsis, ABA receptors and three core components have been described. These include PYR/PYL/RCAR protein family members of the type 2C protein serine/threonine phosphatase (PP2C group A) and class III SNF-1-related protein kinase 2 (SnRK2) families (Mustilli et al, 2002;Yoshida et al, 2006;Fujii et al, 2007;Umezawa et al, 2009;Vlad et al, 2009;Lim et al, 2022). Plant growth under ideal conditions is promoted by SnRK2 kinases in the absence of ABA (Hasan et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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The Bro1-like domain-containing protein, AtBro1, modulates growth and abiotic stress responses in Arabidopsis

2023

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Abscisic acid (ABA) affects plant physiology by altering gene expression, enabling plants to adapt to a wide range of environments. Plants have evolved protective mechanisms to allow seed germination in harsh conditions. Here, we explore a subset of these mechanisms involving the AtBro1 gene, which encodes one of a small family of poorly characterised Bro1-like domain-containing proteins, in Arabidopsis thaliana plants subjected to multiple abiotic stresses. AtBro1 transcripts were upregulated by salt, ABA and mannitol stress, while AtBro1-overexpression lines demonstrated robust tolerance to drought and salt stress. Furthermore, we found that ABA elicits stress-resistance responses in loss-of-function bro1-1 mutant plants and AtBro1 regulates drought resistance in Arabidopsis. When the AtBro1 promoter was fused to the β-glucuronidase (GUS) gene and introduced into plants, GUS was expressed mainly in rosette leaves and floral clusters, especially in anthers. Using a construct expressing an AtBro1-GFP fusion protein, AtBro1 was found to be localized in the plasma membrane in Arabidopsis protoplasts. A broad RNA-sequencing analysis revealed specific quantitative differences in the early transcriptional responses to ABA treatment between wild-type and loss-of-function bro1-1 mutant plants, suggesting that ABA stimulates stress-resistance responses via AtBro1. Additionally, transcripts levels of MOP9.5, MRD1, HEI10, and MIOX4 were altered in bro1-1 plants exposed to different stress conditions. Collectively, our results show that AtBro1 plays a significant role in the regulation of the plant transcriptional response to ABA and the induction of resistance responses to abiotic stress.

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Section: Discussionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

The Bro1-like domain-containing protein, AtBro1, modulates growth and abiotic stress responses in Arabidopsis

2023

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“…Abscisic acid (ABA) is another plant hormone associated with stress tolerance in plants. ABA levels increase under salt and drought stress in plants [86]. Our analysis identified a gene encoding an abscisic acid-and stress-induced protein (Contig8961_at) in the leaves of 20-40 by both salt (3h) and PEG (3h,27h).…”

Section: Expression Profiling Of Wild Barley Accessions Under Salt An...mentioning

confidence: 90%

Expression Profiling of Salt- And Drought-Stress Responsive Genes in Wild Barley (Hordeum spontaneum L.)

Bakır,

Altınkut Uncuoğlu,

Yüksel Özmen

et al. 2023

Preprint

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Drought and salinity are among the most important abiotic stress factors that limit the productivity in economically important plants. Although genes from wild relatives or unadapted germplasm have often been used for stress tolerance breeding, only few studies have examined wild relatives to understand the genetic and molecular basis of stress tolerance. In this study, we monitored the changes in gene expression profiles of leaf and root tissues of two wild barleys (Hordeum spontaneum L.) accessions from Israel after treatment with salt (NaCl) and drought (PEG) stress. We identified a total of 641 differentially expressed genes across 24 conditions (2 accessions, 2 stress conditions, 2 tissues and 3 time points). Our gene expression analyses revealed large numbers of differentially expressed genes in different accessions by different stress treatments. In addition, the number of genes altered in the leaves of stress-exposed plants of both genotypes was higher than those altered in the roots. Interestingly, there was a relatively little overlap between the leaf and the root stress-responsive gene expression patterns, suggesting that different stress-associated processes might be operating in these tissues during stress adaptation. Overall, our results revealed a number of candidate genes and plant processes associated with stress tolerance in wild barley. Wild barley would be a useful source of new genetic variation for drought and salt stress tolerance. Our results could provide new insights into the mechanisms of drought and salt stress tolerance in wild barley and should be useful for genetic improvement of salt-drought tolerance in cultivated barley.

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“…Owing to the massive changes in gene expression and the crucial phosphorylation events involved, research on the ABA pathway has focused primarily on transcriptional and posttranslational processes (Fujita et al., 2011; Lim et al., 2022; Song et al., 2016; Yang et al., 2017). Yet, our understanding of plant stress responses is quickly expanding to encompass regulation at the RNA level.…”

Section: Introductionmentioning

confidence: 99%

Alternative splicing as a driver of natural variation in abscisic acid response

Díez,

Szakonyi,

Lozano‐Juste

et al. 2024

The Plant Journal

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SUMMARYAbscisic acid (ABA) is a crucial player in plant responses to the environment. It accumulates under stress, activating downstream signaling to implement molecular responses that restore homeostasis. Natural variance in ABA sensitivity remains barely understood, and the ABA pathway has been mainly studied at the transcriptional level, despite evidence that posttranscriptional regulation, namely, via alternative splicing, contributes to plant stress tolerance. Here, we identified the Arabidopsis accession Kn‐0 as less sensitive to ABA than the reference Col‐0, as shown by reduced effects of the hormone on seedling establishment, root branching, and stomatal closure, as well as by decreased induction of ABA marker genes. An in‐depth comparative transcriptome analysis of the ABA response in the two variants revealed lower expression changes and fewer genes affected for the least ABA‐sensitive ecotype. Notably, Kn‐0 exhibited reduced levels of the ABA‐signaling SnRK2 protein kinases and lower basal expression of ABA‐reactivation genes, consistent with our finding that Kn‐0 contains less endogenous ABA than Col‐0. ABA also markedly affected alternative splicing, primarily intron retention, with Kn‐0 being less responsive regarding both the number and magnitude of alternative splicing events, particularly exon skipping. We find that alternative splicing introduces a more ecotype‐specific layer of ABA regulation and identify ABA‐responsive splicing changes in key ABA pathway regulators that provide a functional and mechanistic link to the differential sensitivity of the two ecotypes. Our results offer new insight into the natural variation of ABA responses and corroborate a key role for alternative splicing in implementing ABA‐mediated stress responses.

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Core Components of Abscisic Acid Signaling and Their Post-translational Modification

Cited by 15 publications

References 146 publications

The Bro1-like domain-containing protein, AtBro1, modulates growth and abiotic stress responses in Arabidopsis

The Bro1-like domain-containing protein, AtBro1, modulates growth and abiotic stress responses in Arabidopsis

Expression Profiling of Salt- And Drought-Stress Responsive Genes in Wild Barley (Hordeum spontaneum L.)

Alternative splicing as a driver of natural variation in abscisic acid response

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