Cordycepin, 3′-Deoxyadenosine, Prevents Rat Hearts from Ischemia/Reperfusion Injury Via Activation of Akt/GSK-3β/p70S6K Signaling Pathway and HO-1 Expression

Park, Eun-Seok; Kang, Do-Hyun; Yang, Min-Kyu; Kang, Jun Chul; Jang, Yong Chang; Park, Jong‐Seok; Kim, Si-Kwan; Shin, Hyung-Jin

doi:10.1007/s12012-013-9232-0

Cited by 53 publications

(37 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For many years, cordycepin has been applied for various medical purposes, including anti-oxidative stress [39], enhancing bone mineral density [18], preventing myocardial ischemia/reperfusion injury [40], alleviating alcoholic hepatotoxicity [41] and protecting neurons against toxic agents [42]. However, whether cordycepin could exert sufficient protective effects on alcohol-induced ONFH has not been determined.…”

Section: Discussionmentioning

confidence: 99%

The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head

Chen¹,

Zhu²,

Xu³

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: Alcohol abuse is known to be a leading risk factor for atraumatic osteonecrosis of the femoral head (ONFH), in which the suppression of osteogenesis plays a critical role. Cordycepin benefits bone metabolism; however, there has been no study to determine its effect on osteonecrosis. Methods: Human bone mesenchymal stem cells (hBMSCs) were identified by multi-lineage differentiation. Alkaline phosphatase (ALP) activity, RT-PCR, western blots, immunofluorescent assay and Alizarin red staining of BMSCs were evaluated. A rat model of alcohol-induced ONFH was established to investigate the protective role of cordycepin against ethanol. Hematoxylin & eosin (H&E) staining and micro-computerized tomography (micro-CT) were performed to observe ONFH. Apoptosis was assessed by TdT-mediated dUTP nick end labeling (TUNEL). Immunohistochemical staining was carried out to detect OCN and COL1. Results: Ethanol significantly suppressed ALP activity, decreased gene expression of OCN and BMP2, lowered levels of RUNX2 protein, and reduced immunofluorescence staining of OCN and COL1 and calcium formation of hBMSCs. However, these inhibitory effects were attenuated by cordycepin co-treatment at concentrations of 1 and 10 µg/mL Moreover, it was revealed that the osteo-protective effect of cordycepin was associated with modulation of the Wnt/β-catenin pathway. In vivo, by micro-CT, TUNEL and immunohistochemical staining of OCN and COL1, we found that cordycepin administration prevented alcohol-induced ONFH. Conclusion: Cordycepin treatment to enhance osteogenesis may be considered a potential therapeutic approach to prevent the development of alcohol-induced ONFH.

show abstract

Section: Discussionmentioning

confidence: 99%

The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head

Chen¹,

Zhu²,

Xu³

et al. 2017

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Wang et al investigated the effects of cordycepin in prevention of focal cerebral ischemic/reperfusion (IR) injury and suggested that cordycepin has a neuroprotective effect in the ischemic brain, which is due to the inhibition of inflammation and increase of antioxidants activity related to lesion pathogenesis [ 23 , 24 ]. So cordycepin could be an attractive therapeutic candidate with oral activity against I/R-associated heart diseases such as myocardial infarction [ 25 ]. Besides, cordycepin showed the obvious analgesic effect through acetic acid-induced abdominal constrictions, hot-plate test, and neurolysin inhibition assay in mice [ 26 ].…”

Section: Chemical Constituents and Their Corresponding Pharmacologmentioning

confidence: 99%

The Chemical Constituents and Pharmacological Actions ofCordyceps sinensis

Liu

Wang

et al. 2015

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Cordyceps sinensis, also called DongChongXiaCao (winter worm, summer grass) in Chinese, is becoming increasingly popular and important in the public and scientific communities. This study summarizes the chemical constituents and their corresponding pharmacological actions of Cordyceps sinensis. Many bioactive components of Cordyceps sinensis have been extracted including nucleoside, polysaccharide, sterol, protein, amino acid, and polypeptide. In addition, these constituents' corresponding pharmacological actions were also shown in the study such as anti-inflammatory, antioxidant, antitumour, antiapoptosis, and immunomodulatory actions. Therefore can use different effects of C. sinensis against different diseases and provide reference for the study of Cordyceps sinensis in the future.

show abstract

“…In addition, the present study also determined that blockade of the autocrine IGF-1 reduced the expression of p-P70S6K. P70S6K is a serine/threonine kinase that contributes to the downstream signaling in the PI3K/Akt//GSK-3β pathway (32). P70S6K may promote cell cycle progression and cell growth through regulating the organization of cytoskeleton (33).…”

Section: Discussionmentioning

confidence: 54%

Blocking of autocrine IGF-1 reduces viability of human umbilical cord mesenchymal stem cells via inhibition of the Akt/Gsk-3β signaling pathway

2018

View full text Add to dashboard Cite

Human umbilical cord mesenchymal stem cells (hUCMSCs) are able to secrete growth factors, such as hepatocyte growth factor, vascular endothelial growth factor and insulin‑like growth factor‑1 (IGF‑1). The secretion of these growth factors by transplanted hUCMSCs have been identified to stimulate the growth of the host cells in the target organs or tissues. The aim of the present study was to investigate the effect of autocrine IGF‑1 on cell viability of hUCMSCs. The expression levels of IGF‑1 and the IGF‑1 receptor (IGF‑1R) in hUCMSCs were identified using immunocytochemistry staining. In order to block autocrine IGF‑1, hUCMSCs were treated with 5 µg/ml αIR‑3, a specific IGF‑1R antibody, for 24 h. The cells cultured in medium without αIR‑3 were used as the control group. Cell viability, apoptosis, cell cycle and the proliferation‑associated proteins were quantified using an MTT assay, flow cytometry and western blotting. The findings of the present study revealed that IGF‑1 and IGF‑1R were positively expressed in hUCMSCs. Treatment with αIR‑3 significantly reduced cell viability and increased apoptosis of hUCMSCs (P<0.01). Cell cycle analysis indicated that the number of cells in the G2/M phase was reduced in the αIR‑3‑treated group compared with the control group. Western blotting revealed that the expression levels of phosphorylated (p)‑protein kinase B (Akt), p‑glycogen synthase kinase 3β (GSK‑3β), p‑p70 S6 kinase and cyclin D1 were markedly reduced and p21 expression was markedly increased in the αIR‑3‑treated group as compared with the control group (P<0.05). However, no significant difference was identified in the p‑extracellular‑signal regulated kinase 1/2 expression when the αIR‑3 treatment group was compared with the control group. (P>0.05). The findings of the present study suggested that the autocrine IGF‑1 from hUCMSCs may be capable of influencing cell viability of hUCMSCs, which may be associated with activation of Akt/GSK‑3β signaling pathway.

show abstract

Cordycepin, 3′-Deoxyadenosine, Prevents Rat Hearts from Ischemia/Reperfusion Injury Via Activation of Akt/GSK-3β/p70S6K Signaling Pathway and HO-1 Expression

Cited by 53 publications

References 36 publications

The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head

The Protective Effect of Cordycepin On Alcohol-Induced Osteonecrosis of the Femoral Head

The Chemical Constituents and Pharmacological Actions ofCordyceps sinensis

Blocking of autocrine IGF-1 reduces viability of human umbilical cord mesenchymal stem cells via inhibition of the Akt/Gsk-3β signaling pathway

Contact Info

Product

Resources

About