Cord blood processing with an automated and functionally closed system

Zingsem, J.; Strasser, Erwin; Weisbach, Volker; Zimmermann, Robert A.; Ringwald, Juergen; Goecke, Tamme W.; Beckmann, Matthias W.; Eckstein, Reinhold

doi:10.1046/j.1537-2995.2003.00398.x

Cited by 33 publications

(41 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The techniques routinely adopted in clinical practice include HPC processing using the Cobe 2991 24 automated device or saline dilution of the bag followed by centrifugation 9 . Nevertheless, these methods need a long manipulation time and are not free from a significant cell loss with a potential negative impact on the graft take 24‐26 . More recently, a new automated separation device (SepaxS‐100 Biosafe SA) has been proposed, showing interesting preliminary results that need be confirmed 27 .…”

mentioning

confidence: 99%

A new automated cell washer device for thawed cord blood units

et al. 2004

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

A new automated cell washer device for thawed cord blood units

et al. 2004

View full text Add to dashboard Cite

show abstract

“…, 2001). Compomat failed to achieve this RBC depletion level as reported by some authors using automatic devices (Zingsem et al. , 2003).…”

Section: Discussionmentioning

confidence: 60%

“…To standardize the process and improve cell recovery, other approaches with automated or semi‐automated systems have also been developed (Armitage et al. , 1999; Zingsem et al. , 2003).…”

Section: Introductionmentioning

confidence: 99%

Automated separation of cord blood units in top and bottom bags using the Compomat G4

Solves¹,

Mirabet²,

Carbonell-Uberos³

et al. 2006

Clin Lab Haematol

View full text Add to dashboard Cite

Cord blood (CB) has become a real alternative source of haematopoietic stem cells for bone marrow reconstitution in a variety of malignant disorders. As a response to this increasing activity, CB banks have been developed to guarantee the quality of processed CB units. Volume reduction of CB units maximizes storage space and also has other advantages. The aim of this study was to develop a program for the volume reduction of CB in the Compomat G4 device. We also compared two different top and bottom systems for CB fractionation (Compomat G4 and Optipress II). We empirically designed three different programs for volume reduction of CB with Compomat G4: two for final BC volume of 41 ml (CB1 and CB2) and the other one for buffy coat (BC) volume of 25 ml (CB3). Significantly worse recoveries were achieved for CB processed with program CB3. A RBC depletion of >or=50%, >or=60% and >or=70% were achieved for 67%, 39% and 9% of all units respectively. When comparing Compomat G4 and Optipress II, total nucleated cell recovery was similar for both methods, while lymphocytes recovery was significantly better for Optipress II.

show abstract

“…In contrary, in the nonsplit high volume CBUs of our study, the obtained recoveries were reduced (59.7% for WBCs and 62.82% for MNCs). The reason for the reduced recoveries in the nonsplit high volume CBUs, seems to be that automatic devices have been developed to process low volume CB collections, which are too low to be processed in other blood cell separators (Zingsem et al. , 2003).…”

Section: Discussionmentioning

confidence: 99%

A strategy of splitting individual high volume cord blood units into two half subunits prior to processing increases the recovery of cells and facilitates ex vivo expansion of the infused haematopoietic progenitor cells in adults

Papassavas

Gioka

Chatzistamatiou

et al. 2008

Int J Lab Hematology

View full text Add to dashboard Cite

This study was designed to maximize the recovery of the desirable cell populations contained in the cord blood (CB) freezing bag, in order to optimize donor selection for adolescents and adults. To evaluate this hypothesis, high volume CB units (CBUs) were categorized into three volume collection groups (120-139, 140-159 and >or=160 ml) and were randomly split before volume reduction into two half low volume CBUs; (a) and (b). Using the SEPAX Cell Processing System, all CBUs were standardized to 26 ml. In 128 high volume split CBUs, the WBC, mononuclear cell and CD34+ cell recoveries were significantly higher (P

show abstract

Cord blood processing with an automated and functionally closed system

Cited by 33 publications

References 34 publications

A new automated cell washer device for thawed cord blood units

A new automated cell washer device for thawed cord blood units

Automated separation of cord blood units in top and bottom bags using the Compomat G4

A strategy of splitting individual high volume cord blood units into two half subunits prior to processing increases the recovery of cells and facilitates ex vivo expansion of the infused haematopoietic progenitor cells in adults

Contact Info

Product

Resources

About