Cord Blood CD16+56−Cells with Low Lytic Activity Are Possible Precursors of Mature Natural Killer Cells

Gaddy, Jay; Broxmeyer, Hal E.

doi:10.1006/cimm.1997.1175

Cited by 120 publications

(112 citation statements)

References 52 publications

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“…-(precursor NK cell) and CD3 -CD16 þ CD56 þ (mature cytotoxic NK cell), a three-colour flow cytometry analysis using anti-CD3 FITC, anti-CD56 PE and anti-CD16 TC was performed, as proposed by Gaddy et al [31]. We observed a higher frequency of circulating NK cells in all groups of chagasic patients (P < 0.05).…”

Section: Cd56mentioning

confidence: 62%

Chagasic Patients with Indeterminate Clinical Form of the Disease have High Frequencies of Circulating CD3⁺CD16^–CD56⁺ Natural Killer T Cells and CD4⁺CD25^High Regulatory T Lymphocytes

et al. 2005

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Several studies have demonstrated that different clinical manifestations of human Chagas' disease are associated with distinct and complex host-parasite relationships directly involving the immune system. In this context, it has been proposed that tissue damage might be more severe in the absence of regulatory mechanisms that involve both innate and adaptive immune responses. Herein, we describe a descriptive phenotypic profile focusing on the frequency of major regulatory T cells [CD4

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Section: Cd56mentioning

confidence: 62%

Chagasic Patients with Indeterminate Clinical Form of the Disease have High Frequencies of Circulating CD3⁺CD16^–CD56⁺ Natural Killer T Cells and CD4⁺CD25^High Regulatory T Lymphocytes

et al. 2005

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“…Ϫ precursor cells to mature CD16 ϩ CD56 ϩ and CD16 Ϫ CD56 ϩ NK cells (5). NK precursor cells are not detected in the T1 assay.…”

Section: Cd56mentioning

confidence: 95%

Evaluation of a Simplified Dual-Platform Flow Cytometric Method for Measurement of Lymphocyte Subsets and T-Cell Maturation Phenotypes in the Population of Nouna, Burkina Faso

Böhler

Klose

et al. 2007

Clin Vaccine Immunol

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In the context of a larger clinical study in Nouna, Burkina Faso, we evaluated a simplified dual-platform (DP) flow cytometric (FCM) method that allows the determination of major lymphocyte subsets in a single test tube. We compared the phenotyping of lymphocytes with DP FCM and simultaneous measurements with standard single-platform (SP) FCM for samples from 177 individuals. Analysis of the comparative measurements revealed that DP FCM systematically underestimates the proportion of NK cells, overestimates the percentage of CD3 ؉ CD8 ؉ lymphocytes, and yields proportions of B cells and CD4 ؉ T cells comparable with the results from SP FCM. Bland-Altman analysis showed a low bias between both methods and an acceptable precision for percent values of CD4 ؉ T cells (bias ؎ precision, ؊1% ؎ 6%) and CD8 ؉ T cells (؊3% ؎ 6%). The absolute cell numbers of all lymphocyte subpopulations, however, were systematically biased towards lower values being obtained by DP FCM. Reference values for the distribution of T-cell maturation phenotypes in 177 healthy adults were calculated using DP FCM. The mean ؎ standard deviation (SD) CD4؉ -to-CD8 ؉ T-cell ratio was 1.61 ؎ 0.61, the mean percentage ؎ SD of CD4 ؉ T cells was 42% ؎ 7%, and that of CD8 ؉ T cells 29% ؎ 7%. Among CD4 ؉ lymphocytes, 28% ؎ 7% were classified as central memory (CD45RA low CCR7 ؉ ), 22% ؎ 10% as naïve (CD45RA high CCR7 ؉ ), 45% ؎ 12% as effector memory (CD45RA low CCR7 ؊ ); and 5% ؎ 3% as terminally differentiated effector memory expressing CD45RA (CD45RA high CCR7 ؊ ). Among CD8 bright lymphocytes, 3% ؎ 2% had a central memory phenotype, 27% ؎ 13% were naïve, 37% ؎ 13% had an effector memory phenotype, and 34% ؎ 12% were terminally differentiated effector memory cells expressing CD45RA.In the years 2004 and 2005, a population-based study was performed in Nouna, Burkina Faso, in order to generate site-and gender-specific reference values for lymphocyte subsets in healthy adults in the context of an expanding program for prevention of mother-to-child transmission of human immunodeficiency virus type 1 (HIV-1) (17). During that study, single-platform (SP) flow cytometry (FCM) was used (9), a method which is not available to most laboratories in developing countries due to its relatively high cost (7).Since lymphocyte differentiation and counting by FCM is needed for immunological monitoring of antiretroviral treatment in resource-limited settings and immunological field studies on cohorts of young infants suffering from diseases other than infection with HIV-1 were planned in our research setting, we wanted to use an FCM test which allows the determination of the complete lymphocyte differential. The test should be reliably performed with low volumes of venous and capillary blood and should be resistant against preanalytic errors. It should be as inexpensive as possible and should be run on a simple flow cytometer equipped with only one laser.In the present study, we evaluated such a simplified dualplatform (DP) FCM method for its clinical use in Nouna. The m...

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“…ϩ phenotype (13,19). Although the role of NK cells in Mycobacterium infections is not completely clear, it has been demonstrated that in the tuberculosis context, the activation of innate immune response in the beginning of the infection, through recruiting of pre-NK cells, could be a linkage between innate and acquired immune response (7).…”

Section: Cd56mentioning

confidence: 99%

Peripheral Blood Mononuclear Cells Immunophenotyping in Pulmonary Tuberculosis Patients before and after Treatment

Barcelos¹,

Martins‐Filho

Guimarães³

et al. 2006

Microbiology and Immunology

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Tuberculosis (TB) is a lung disease caused by Mycobacterium tuberculosis. The interaction between the bacillus and the host may lead to a protective cellular immune response. In the present study, we propose the "in vitro" evaluation of this cellular immune response in patients with tuberculosis before and after chemotherapic treatment. Eleven patients with TB and 9 asymptomatic subjects with tuberculin skin test negative (TST؊) (purified protein derivative (PPD) ≤10 mm) were evaluated. The peripheral lymphocytes of the subjects were analyzed utilizing the following surface markers: CD3؉ and HLA-DR

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Cord Blood CD16+56−Cells with Low Lytic Activity Are Possible Precursors of Mature Natural Killer Cells

Cited by 120 publications

References 52 publications

Chagasic Patients with Indeterminate Clinical Form of the Disease have High Frequencies of Circulating CD3⁺CD16^–CD56⁺ Natural Killer T Cells and CD4⁺CD25^High Regulatory T Lymphocytes

Chagasic Patients with Indeterminate Clinical Form of the Disease have High Frequencies of Circulating CD3⁺CD16^–CD56⁺ Natural Killer T Cells and CD4⁺CD25^High Regulatory T Lymphocytes

Evaluation of a Simplified Dual-Platform Flow Cytometric Method for Measurement of Lymphocyte Subsets and T-Cell Maturation Phenotypes in the Population of Nouna, Burkina Faso

Peripheral Blood Mononuclear Cells Immunophenotyping in Pulmonary Tuberculosis Patients before and after Treatment

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Cord Blood CD16+56−Cells with Low Lytic Activity Are Possible Precursors of Mature Natural Killer Cells

Cited by 120 publications

References 52 publications

Chagasic Patients with Indeterminate Clinical Form of the Disease have High Frequencies of Circulating CD3+CD16–CD56+ Natural Killer T Cells and CD4+CD25High Regulatory T Lymphocytes

Chagasic Patients with Indeterminate Clinical Form of the Disease have High Frequencies of Circulating CD3+CD16–CD56+ Natural Killer T Cells and CD4+CD25High Regulatory T Lymphocytes

Evaluation of a Simplified Dual-Platform Flow Cytometric Method for Measurement of Lymphocyte Subsets and T-Cell Maturation Phenotypes in the Population of Nouna, Burkina Faso

Peripheral Blood Mononuclear Cells Immunophenotyping in Pulmonary Tuberculosis Patients before and after Treatment

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Chagasic Patients with Indeterminate Clinical Form of the Disease have High Frequencies of Circulating CD3⁺CD16^–CD56⁺ Natural Killer T Cells and CD4⁺CD25^High Regulatory T Lymphocytes

Chagasic Patients with Indeterminate Clinical Form of the Disease have High Frequencies of Circulating CD3⁺CD16^–CD56⁺ Natural Killer T Cells and CD4⁺CD25^High Regulatory T Lymphocytes