Corazonin receptor signaling in ecdysis initiation

Kim, Young-Joon; Spalovská-Valachová, Ivana; Cho, Kook-Ho; Žitňanová, Inka; Park, Yoonseong; Adams, Michael E.; Žitňan, Dušan

doi:10.1073/pnas.0305291101

Cited by 181 publications

(159 citation statements)

References 38 publications

Supporting

Mentioning

146

Contrasting

Unclassified

Order By: Relevance

“…The process by which the adult emerges is called eclosion. Similar to larval ecdysis, eclosion behavior is controlled by time of day and driven by additional endocrine factors such as eclosion hormone (EH) (Truman, 1971;Kim et al, 2004).…”

Section: Hormonal Regulation Of Molting and Metamorphosismentioning

confidence: 99%

Nutritional sensitivity of fifth instar prothoracic glands in the tobacco hornworm, Manduca sexta

Walsh

Smith

2011

Journal of Insect Physiology

View full text Add to dashboard Cite

The tobacco hornworm, Manduca sexta, has been a useful model for studying insect endocrinology. In M. sexta the majority of growth occurs during the fifth (final) larval stage. This growth is concurrent with endocrine changes that lead to a metamorphic molt. Past research has focused on the interplay of three growth hormones: juvenile hormone (JH), prothoracicotropic hormone (PTTH) and ecdysone, in regulating metamorphosis. The prothoracic glands, source of ecdysteroid molting hormones in insects, play a crucial role in coordinating growth. Insulin/insulin-like growth factor (IGF) signaling has been shown to regulate growth and secretion of steroidogenic tissues in both vertebrates and invertebrates, but it had yet to be well-characterized in M. sexta.In this dissertation I examined the role of insulin-directed growth in prothoracic glands during both normal larval development and in response to nutrient deprivation. In particular, I predicted that in response to a reduction in nutrients, the prothoracic glands would stop growing and ecdysone secretion would be reduced. Changes were assessed in actin abundance and in two nutritionally sensitive transcripts, insulin receptor and 4EBP.Actin abundance in prothoracic glands increased in the first four days of this stage, demo nstrating significant growth. Actin decreased in glands from starved larvae, indicating a cessation of growth. Both insulin receptor and 4EBP transcript were present during normal larval development. As expected, both insulin receptor and 4EBP transcript increased in response to starvation. This research was the first demonstration of insulin signaling in prothoracic glands of fifth instar M. sexta larvae, and revealed nutritional sensitivity of the prothoracic glands. 5Finally, ecdysone assays were conducted to determine the impact of nutrition on glandular secretory capacity. Starved larvae were unable to secrete as much ecdysone as fed controls. Injections of insulin were unable to rescue the ability of the glands to secrete high levels of ecdysone. This demonstrated that although insulin signaling was correlated with normal growth of the glands, insulin is not sufficient to impact the ability of the glands to secrete ecdysone in the absence of nutrie nts. 6 This thesis is dedicated to my parents,Robert and Angela Walsh, whose love and support made this dissertation possible.

show abstract

Section: Hormonal Regulation Of Molting and Metamorphosismentioning

confidence: 99%

Nutritional sensitivity of fifth instar prothoracic glands in the tobacco hornworm, Manduca sexta

Walsh

Smith

2011

Journal of Insect Physiology

View full text Add to dashboard Cite

show abstract

“…One line (WTA11-CHO) exhibited stable expression of aequorin and G α16 (Euroscreen), and the other was a wild type line (CHO-K1). WTA11-CHO cells were transiently transfected with ORFs of HevPBANR-A, -B, or -C [22], whereas CHO-K1 cells were transfected with two plasmid containing ORFs of HevPBANR-C and codon-optimized aequorin as described [12,31]. Procedures used for CHO cell transfection and receptor assay were described previously [12,13,22].…”

Section: Cho Cell Expressionmentioning

confidence: 99%

The pheromone biosynthesis activating neuropeptide (PBAN) receptor of Heliothis virescens: Identification, functional expression, and structure–activity relationships of ligand analogs

et al. 2008

Self Cite

View full text Add to dashboard Cite

Pheromone biosynthesis activating neuropeptide (PBAN) promotes synthesis and release of sex pheromones in moths. We have identified and functionally expressed a PBAN receptor from Heliothis virescens (HevPBANR) and elucidated structure-activity relationships of PBAN analogs. Screening of a larval CNS cDNA library revealed three putative receptor subtypes and nucleotide sequence comparisons suggest they are produced through alternative splicing at the 3'-end. RT-PCR amplified preferentially HevPBANR-C from female pheromone glands. CHO cells expressing HevPBANR-C are highly sensitive to PBAN and related analogs, especially those sharing the C-terminal pentapeptide core, FXPRLamide (X = T, S or V). Alanine replacements in the C-terminal hexapeptide (YFTPRLamide) revealed the relative importance of each residue in the active core as follows, R 5 >L 6 >F 2 ≫P 4 >T 3 ≫Y 1 . This study provides a framework for the rational design of PBANR-specific agonists and/or antagonists that could be exploited for disruption of reproductive function in agriculturally important insect pests.

show abstract

“…58) It is best known however as an initiator of the ecdysis behavior, by stimulating the release of preecdysis-and ecdysis-trigerring hormone (PETH, ETH, see below). 59,60) CRZ shares sequence homology with both AKH and the hypertrehalosemic hormone (HTH) to which it is probably ancestrally related. The CRZ receptor has been isolated in both Drosophila (DrmCRZR) and M. sexta (MasCRZR).…”

Section: Corazonin (Crz)mentioning

confidence: 99%

“…59,60,[67][68][69][70] To truly understand the ecdysis behavior we have to examine the raison d'être of the molting process. Insects and crustaceans have a rigid exoskeleton and therefore to accommodate growth they have to periodically shed the exoskeleton and replace it with a new one.…”

Section: Ecdysis Motor Behavior Controlling Peptides: Ptth Eh Pethmentioning

confidence: 99%

“…The new cuticle is elaborated, sclerotized and hardened with the help of another neurohormone, bursicon (a 173 amino acid protein belonging to the cystine knot family of growth factors), which is coexpressed with CCAP in the bilateral ventral neurons in the central nervous system of Drosophila. 59,[67][68][69][70]73) The ecdysis cascade begins with the ventro-median cells (VM cells) secreting the eclosion hormone (EH) in the presence of 20E, which is stored in the proctodeal nerves (proct. nerve) which serves as the neurohemal organ and is released in the absence of 20E (Fig.…”

Section: Ecdysis Motor Behavior Controlling Peptides: Ptth Eh Pethmentioning

confidence: 99%

See 1 more Smart Citation

Molecular physiology of crustacean and insect neuropeptides

Mercier¹,

Doucet

Retnakaran³

2007

J. Pestic. Sci.

View full text Add to dashboard Cite

One of the principal hallmarks of a living organism is its ability to respond to stimuli. As the unicellular organisms evolved into multicellular metazoans, the external signals perceived had to be transduced to several other cells requiring a neuronal network with its accompanying biochemical neurotransmitters. The progressive increase in complexity of the organisms necessitated the need for an integrated signal transducing system collecting external sensory stimuli and sending them to effector systems such that a dynamic equilibrium or homeostasis, which is essential for survival, could be maintained. During the course of evolution many advanced sense organs were developed to detect various parameters in the environment, and concomitantly different internal effector systems were formed to respond to these signals received from outside to maintain homeostasis. Coordination was achieved with a network of electrical conductors in the form of an arborized nervous system and a variety of hydrophilic biochemicals that acted as neurotransmitters carrying the sensory signals to the internal effector systems. As the animal became increasingly complex, a sophisticated neurotransmission system catering to the spatial and temporal needs during growth, development and reproduction had to be in place. Parts of the nervous tissue in many locations took on a secretory role and released protein signal transducers, the neuropeptides. A panoply of such neuropeptides exist in all organisms functioning as neurohormones, neurotransmitters or neuromodulators. Aspects of neuropeptide structure and function in insects and crustaceans have been reviewed by various authors. [1][2][3][4][5][6][7][8][9][10] The neuropeptides are ligands to their cognate receptors in the ef- Organisms have to constantly respond to environmental conditions to maintain a status of dynamic homeostasis for survival. As single celled organisms evolved into multicellular animals, intercellular and inter-organ communications became indispensable not only to orchestrate homeostasis but also to control the many events punctuating metazoan development. To accomplish this need, a signal transduction system was evolved, consisting of an arborized network of nerves as well as a collection of oligopeptide neurotransmitters (neuropeptides) along with their cognate receptors. We review here the current state of our understanding of the physiology of neuropeptides in the most species-rich group of animals, the crustaceans and insects. The vast majority of neuropeptides signal through cell-surface guanosine-protein coupled receptors (GPCRs). These neuropeptide-receptor systems control a variety of physiological functions, for instance the numerous involuntary movements of internal ducts that are precisely timed for transporting reproductive, digestive and secretory materials. The rigid exoskeleton in Crustaceans and Insects require periodic molts to accomplish growth and metamorphosis and this is harmoniously regulated by a cascade of neuropeptides. Neuropeptides also regul...

show abstract

Corazonin receptor signaling in ecdysis initiation

Cited by 181 publications

References 38 publications

Nutritional sensitivity of fifth instar prothoracic glands in the tobacco hornworm, Manduca sexta

Nutritional sensitivity of fifth instar prothoracic glands in the tobacco hornworm, Manduca sexta

The pheromone biosynthesis activating neuropeptide (PBAN) receptor of Heliothis virescens: Identification, functional expression, and structure–activity relationships of ligand analogs

Molecular physiology of crustacean and insect neuropeptides

Contact Info

Product

Resources

About