CoQ10 increases mitochondrial mass and polarization, ATP and Oct4 potency levels, and bovine oocyte MII during IVM while decreasing AMPK activity and oocyte death

Abstract

The data suggest that CoQ10 improves mitochondrial function in IVM where unwanted stress, higher AMPK activity, and Oct4 potency loss are induced.

Supplementation with exogenous coenzyme Q10 to media for in vitro maturation and embryo culture fails to promote the developmental competence of porcine embryos

“…The supplementation with CoQ10 to the IVM medium failed to increase not only maturation but also fertilization rates and the subsequent embryo development. These results are in disagreement with those reported in bovine, where CoQ10 treatment for 24 hr of maturation increased oocyte survival and the percentage of oocytes achieving the MII stage compared with control oocytes (Abdulhasan et al., ). These authors indicated that the improved conditions of maturation in vitro counteracted oxidative stress and that the CoQ10 increased ATP production and decreased AMPK activity, which improved maturation outcomes.…”

“…The coenzyme Q10 (CoQ10) is an essential cofactor of the electron transport chain present especially within the inner mitochondrial membrane where it serves as a potent antioxidant playing a critical role in protecting the cell in question against mitochondrial dysfunction mediated by oxidative stress (Hernández‐Camacho, Bernier, López‐Lluch, & Navas, ). In addition, CoQ10 promotes the synthesis of adenosine triphosphate (ATP), thus playing a crucial role in the activation of the fertilized oocyte towards further embryo development (Abdulhasan et al., ) owing to the fact that the mitochondria of the zygote are all derived from the oocyte and thus constitute the major source of ATP during the development of the pre‐implantation embryos (Babayev & Seli, ).…”

“…Despite these fundamental functions, little is known about the eventual actions of exogenous CoQ10 on the in vitro development of oocytes and embryos, and basically few, if any, experimental evidence has been presented for its beneficial or deleterious effects. In bovine, addition of exogenous CoQ10 to the maturation medium increased nuclear maturation rates and viability of oocytes (Abdulhasan et al., ). The effects of exogenous CoQ10 under moderate‐heat stress not only improved mitochondrial distribution, membrane polarization and differential expression of genes involved in the mitochondrial electron transport chain, but also improved oocyte developmental competence (Gendelman & Roth, ).…”

Supplementation of maturation medium with CoQ10 enhances developmental competence of ovine oocytes through improvement of mitochondrial function

“…The results of MitoTracker staining revealed that diffused pattern of mitochondrial distribution, as well as mitochondrial mass, were more pronounced in matured oocytes upon treatment with 30 µM CoQ10. Furthermore, the results of JC1 staining suggested an improvement in mitochondrial membrane potential upon supplementation with 30 µM CoQ10 Which were consistent to previous reports (Abdulhasan et al, ; Gendelman & Roth, ; Liang, Niu, Shin, & Cui, ).…”

“…of JC1 staining suggested an improvement in mitochondrial membrane potential upon supplementation with 30 µM CoQ10 Which were consistent to previous reports(Abdulhasan et al, 2017;Gendelman & Roth, 2012;Liang, Niu, Shin, & Cui, 2017).Beside its role in the maintenance of cellular energy, mitochondria contributes to proper chromosomal segregation. This takes place during meiotic divisions and after oocyte activation by sperm(Eichenlaub-Ritter, Vogt, Yin, & Gosden, 2004).…”