Copy-number of broad host-range plasmid R1162 is regulated by a small RNA

Kim, Kyunghoon; Meyer, Richard J.

doi:10.1093/nar/14.20.8027

Cited by 43 publications

(29 citation statements)

References 42 publications

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“…Studies of this sort should provide additional insights into the reasons for the broad-host-range, promiscuous character of the IncQ-like plasmids. In addition, Kim and Meyer (41) reported that a 75-bp antisense RNA molecule transcribed from DNA immediately upstream of the repA gene of IncQ plasmid R1162 regulated the plasmid copy number. Investigations into the possibility that antisense RNA may play a role in the regulation of other members of the IncQ-family have been neglected.…”

Section: Summary and Future Directionsmentioning

confidence: 99%

Comparative Biology of IncQ and IncQ-Like Plasmids

Rawlings

Tietze

2001

Microbiol Mol Biol Rev

136

124

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Plasmids belonging to Escherichia coli incompatibility group Q are relatively small (approximately 5 to 15 kb) and able to replicate in a remarkably broad range of bacterial hosts. These include gram-positive bacteria such as Brevibacterium and Mycobacterium and gram-negative bacteria such as Agrobacterium, Desulfovibrio, and cyanobacteria. These plasmids are mobilized by several self-transmissible plasmids into an even more diverse range of organisms including yeasts, plants, and animal cells. IncQ plasmids are thus highly promiscuous. Recently, several IncQ-like plasmids have been isolated from bacteria found in environments as diverse as piggery manure and highly acidic commercial mineral biooxidation plants. These IncQ-like plasmids belong to different incompatibility groups but have similar broad-host-range replicons and mobilization properties to the IncQ plasmids. This review covers the ecology, classification, and evolution of IncQ and IncQ-like plasmids

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Section: Summary and Future Directionsmentioning

confidence: 99%

Comparative Biology of IncQ and IncQ-Like Plasmids

Rawlings

Tietze

2001

Microbiol Mol Biol Rev

136

124

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“…6). In this regard, it is interesting to note that, similar to pVT736-1, the RBS of gene replA, encoding one of the replication proteins of E. coli plasmid R1162, also is located in the loop of a stable hairpin (16). As described for FIG.…”

Section: Discussionmentioning

confidence: 93%

“…pVT736-1, an antisense RNA molecule has been identified that binds to the ReplA mRNA of R1162, in a region that overlaps with the SD sequence (16). Although this countertranscript is believed to control ReplA expression, loss of the antisense RNA increases the copy number of R1162 only by 30 to 40% (16).…”

Section: Discussionmentioning

confidence: 99%

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Transcriptional analysis of rolling circle replicating plasmid pVT736-1: evidence for replication control by antisense RNA

Galli

LeBlanc

1995

J Bacteriol

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Several plasmids have been described in Actinobacillus actinomycetemcomitans, a gram-negative coccobacillus. Recently, the nucleotide sequence of pVT736-1, a cryptic plasmid of A. actinomycetemcomitans VT736, was determined. This plasmid possesses all the features necessary for rolling circle replication. The present study involved a transcriptional analysis of pVT736-1. Results of Northern (RNA) blot analyses and primer extension studies indicated that the two open reading frames identified in pVT736-1 are each preceded by at least one promoter. Expression of these promoters varied with growth phase. In addition, an antisense RNA (Cop RNA) appeared to control the synthesis of the putative replication protein. To our knowledge, this is the first rolling circle replicating plasmid isolated from a gram-negative organism that has been subjected to such detailed analysis.Actinobacillus actinomycetemcomitans is a gram-negative, capnophilic coccobacillus associated with various forms of periodontitis (34). The presence of plasmid DNA in A. actinomycetemcomitans has been reported previously (18,23). Characterization of these plasmids is important in terms of their possible carriage and dissemination of virulence genes and for the development of genetic and molecular tools applicable to A. actinomycetemcomitans. Recently, the nucleotide base sequence of pVT736-1, a cryptic plasmid of A. actinomycetemcomitans VT736, was determined (13). Computer analysis revealed the presence of two open reading frames encoding predicted proteins of 293 (ORF1) and 95 (ORF2) amino acids (13). Evidence that pVT736-1 replicates via a rolling circle mode included the presence of single-stranded DNA (ssDNA) in cells and in cell-free supernatant and the identification of conserved sequence motifs in the predicted ORF1 protein that are typical of initiator (Rep) proteins associated with this type of replication (13).Rolling circle replicating (RCR) plasmids have been identified primarily in gram-positive bacteria. Novick (21) has divided these plasmids into four groups, represented by pT181, pUB110/pC194, pLS1/pE194, and pSN2. To date, the regulation of replication has been well characterized for only two RCR plasmids, the staphylococcal plasmid pT181 (for a review, see reference 21) and the streptococcal plasmid pLS1 (6). Plasmid-encoded products control plasmid copy number in both cases: two antisense RNAs for pT181 (17) and an antisense RNA and a small repressor protein for pLS1 (6). The target of the antisense transcripts in both instances is the mRNA of the plasmid-specific initiator protein (Rep protein), but the mode of antisense action is different. Binding of the antisense RNA induces premature termination of transcription (attenuation) in the Rep RNA of pT181 (22), whereas the countertranscript inhibits translation of the target messenger of pLS1 by blocking the ribosome binding site (RBS) (6).Recently, a number of RCR plasmids have been reported in gram-negative bacteria (reviewed in references 7 and 27). With the exception of pVT736...

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“…ctRNA species (Kim & Meyer, 1986), did not exert incompatibility, this suggested that the incompatibility determinant could be localized to the RSF1010 repAC operon (Fig. 1).…”

Section: Identifying the Incq Plasmid Locus Expressing Incompatibilitmentioning

confidence: 94%

Evolution of compatible replicons of the related IncQ-like plasmids, pTC-F14 and pTF-FC2

Gardner

Rawlings

2004

Microbiology

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Two closely related but compatible plasmids of the IncQ-2a and IncQ-2b groups, pTF-FC2 and pTC-F14, were discovered in two acidiphilic chemolithotrophic bacteria. Cross-complementation and cross-regulation experiments by the replication proteins were carried out to discover what changes were necessary when the plasmids evolved to produce two incompatibility groups. The requirement of a pTC-F14 oriV for a RepC DNA-binding protein was plasmid specific, whereas the requirement for the RepA helicase and RepB primase was less specific and could be complemented by the IncQ-2a plasmid pTC-FC2, and the IncQ-1b plasmid pIE1108. None of the IncQ-1a plasmid replication proteins could complement the pTC-F14 oriV, and pTC-F14 and RSF1010 were incompatible. This incompatibility was associated with the RepC replication protein and was not due to iteron incompatibility. Replication of pTC-F14 took place from a 5?7 kb transcript that originated upstream of the mobB gene located within the region required for mobilization. A pTC-F14 mobB-lacZ fusion was regulated by the pTC-F14 repB gene product and was plasmid specific, as it was not regulated by the RepB proteins of pTF-FC2 or the IncQ-1a and IncQ-1b plasmids. Plasmid pTC-F14 appears to have evolved independently functioning iterons and a plasmid-specific RepC-binding protein; it also has a major replication transcript that is independently regulated from that of pTF-FC2. However, the RepA and RepB proteins have the ability to function with either replicon.

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Copy-number of broad host-range plasmid R1162 is regulated by a small RNA

Cited by 43 publications

References 42 publications

Comparative Biology of IncQ and IncQ-Like Plasmids

Comparative Biology of IncQ and IncQ-Like Plasmids

Transcriptional analysis of rolling circle replicating plasmid pVT736-1: evidence for replication control by antisense RNA

Evolution of compatible replicons of the related IncQ-like plasmids, pTC-F14 and pTF-FC2

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