Coptisine-induced apoptosis in human colon cancer cells (HCT-116) is mediated by PI3K/Akt and mitochondrial-associated apoptotic pathway

Han, Bing; Pu, Jun; Li, Zhaoxing; Ye, Yu; Huang, Tao; Yang, Xiaoli; Li, Xuegang

doi:10.1016/j.phymed.2017.12.027

Cited by 72 publications

(46 citation statements)

References 28 publications

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“…For instance, the ethanol extract of Moringa oleifera leaf initiated apoptosis by downregulation of Bcl-2 and Bcl-xL and up-regulation of Bax and caspase-3 [31]. Coptisine induces apoptosis of human colon cancer cells via mitochondrial-associated apoptotic pathway mediated by PI3K/Akt [32]. In the present study, OPE was found to enhance apoptosis in HepG2 and SMMC-7721 cells.…”

Section: Discussionsupporting

confidence: 55%

Ethanol extract of Ophiorrhiza pumila suppresses liver cancer cell proliferation and migration

et al. 2020

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Background: Ophiorrhiza pumila, belonging to the genus Ophiorrhiza (Rubiaceae), is distributed throughout tropical and subtropical Asia. In this study, we evaluated for the first time the anti-proliferation and anti-migration effects of ethanol extract of O. pumila (OPE) on HepG2 and SMMC-7721 cells, and explored the related mechanism.Methods: OPE was prepared by percolation with 95% ethanol and its main compounds were analyzed by HPLC-MS 2 . The anti-proliferation effect of OPE was evaluated by the CCK-8 assay and colony formation assay. Cell cycle distribution, apoptosis, and reactive oxygen species (ROS) level were detected by flow cytometry. Migration and invasion abilities were detected by Transwell migration/invasion assays. The expression of correlated proteins was determined using western blotting.Results: A total of 5 tentative compounds were identified from OPE, including pumiloside, deoxypumiloside, camptothecin, aknadinine, and β-stigmasterol. OPE displayed strong cytostatic effects on HepG2 and SMMC-7721 cells. OPE induced G2/M phase cell cycle arrest, increased apoptosis, and augmented ROS production in these cell lines. In addition, OPE possessed a significant inhibition on cell migration and invasion by reduction of MMP-9 and MMP-2 expression. Moreover, OPE significantly suppressed the phosphorylation of p65. Conclusions:Our data showed that OPE suppresses liver cancer cell proliferation and migration, which is possibly involved with the inhibition of the NF-κB pathway.

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Section: Discussionsupporting

confidence: 55%

Ethanol extract of Ophiorrhiza pumila suppresses liver cancer cell proliferation and migration

et al. 2020

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“…29,30 Coptisine (0-28.11 μmol/L, 24 hours; 0-75 μmol/L, 48 hours) induced HCT-116 cell cycle arrest in G1 phase as well as decreased expressions of CDK4, CDK2, cyclin E and cyclin C, which were key genes of G1/S phase. 31,32 Similar anti-cancer property of coptisine was observed in liver 33,34 In the next study, authors revealed that coptisine and NCI•H1650 cells. [38][39][40] Several studies have established that coptisine exerts ameliorative effect on cancer in in vivo model.…”

Section: Anti -C An Cer Propert Ymentioning

confidence: 61%

“…Cell circle interphase consists of G1, S and G2 phases, respectively, ensuring DNA synthesis preparation, DNA replication and mitosis preparation . Coptisine (0‐28.11 μmol/L, 24 hours; 0‐75 μmol/L, 48 hours) induced HCT‐116 cell cycle arrest in G1 phase as well as decreased expressions of CDK4, CDK2, cyclin E and cyclin C, which were key genes of G1/S phase …”

Section: Anti‐cancer Propertymentioning

confidence: 99%

Coptisine from Coptis chinensis exerts diverse beneficial properties: A concise review

Luo

Deng

et al. 2019

J Cellular Molecular Medi

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Coptisine is a natural small‐molecular compound extracted from Coptis chinensis (CC) with a history of using for thousands of years. This work aimed at summarizing coptisine's activity and providing advice for its clinical use. We analysed the online papers in the database of SciFinder, Web of Science, PubMed, Google scholar and CNKI by setting keywords as ‘coptisine’ in combination of ‘each pivotal pathway target’. Based on the existing literatures, we find (a) coptisine exerted potential to be an anti‐cancer, anti‐inflammatory, CAD ameliorating or anti‐bacterial drug through regulating the signalling transduction of pathways such as NF‐κB, MAPK, PI3K/Akt, NLRP3 inflammasome, RANKL/RANK and Beclin 1/Sirt1. However, we also (b) observe that the plasma concentration of coptisine demonstrates obvious non‐liner relationship with dosage, and even the highest dosage used in animal study actually cannot reach the minimum concentration level used in cell experiments owing to the poor absorption and low availability of coptisine. We conclude (a) further investigations can focus on coptisine's effect on caspase‐1‐involved inflammasome assembling and pyroptosis activation, as well as autophagy. (b) Under circumstance of promoting coptisine availability by pursuing nano‐ or microrods strategies or applying salt‐forming process to coptisine, can it be introduced to clinical trial.

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“…The in vivo results of coptisine chloride remarkably suppress the parasitemia of greater than 80 %, and the density of parasitemia was signi cantly lower than the negative control (P <.01). As previously reported, coptisine had wide verities of activities such as inducing apoptosis in human colon cancer [51], inhibiting in ammatory response of mast cell [52], and antidiabetic [53]. However, this is the rst report of the coptisine chloride to have an in vivo antimalarial activity.…”

Section: Discussionmentioning

confidence: 80%

Antimalarial activity of traditional Kampo medicine Coptis Rhizome extract and its major active compounds

Teklemichael

Mizukami

Toume

et al. 2020

Preprint

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Background: The herbal medicine has been a rich source of new drugs exemplified by quinine and artemisinin. In this study, examined a variety of Japanese traditional herbal medicine (Kampo) for their potential antimalarial activities. Methods: We designed a comprehensive screening to identify novel antimalarial drugs from a library of Kampo herbal extracts (n = 120) and related compounds (n=96). The antimalarial activity was initially evaluated in vitro against chloroquine/mefloquine-sensitive (3D7) and -resistant (Dd2) strains of Plasmodium falciparum . The cytotoxicity was also evaluated using primary Adult Mouse Brain cells. After being selected through the first in vitro assay, positive extracts and compounds were examined for possible in vivo antimalarial activity. Results: Out of 120 herbal extracts, Coptis Rhizome showed the highest antimalarial activity (IC 50 1.9 µg/mL of 3D7 and 4.85 µg/mL of Dd2) with a high selectivity index (SI) > 263 (3D7) and > 103 (Dd2). Three major chlorinated compounds (coptisine, berberine, and palmatine) related to Coptis Rhizome also showed antimalarial activities with IC 50 1.1, 2.6, and 6.0 µM (against 3D7) and 3.1, 6.3, and 11.8 µM (against Dd2), respectively. Among them, coptisine chloride exhibited the highest antimalarial activity (IC 50 1.1 µM against 3D7 and 3.1 µM against Dd2) with SI of 37.8 and 13.2, respectively. . Finally, the herbal extract of Coptis Rhizome and its major active compound coptisine chloride exhibited significant antimalarial activity in mice infected with P. yoelii 17X strain with respect to its activity on parasite suppression consistently from day 3 to day 7 post-challenge. The effect ranged from 50.38 to 72.13% (P <.05) for Coptis Rhizome and from 81 to 89% (P <.01) for coptisine chloride. Conclusion: Coptis Rhizome and its major active compound coptisine chloride showed promising antimalarial activity against chloroquine-sensitive (3D7) and -resistant (Dd2) strains in vitro as well as in vivo mouse malaria model. Thus Kampo herbal medicine is a potential natural resource for novel antipathogenic agents.

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Coptisine-induced apoptosis in human colon cancer cells (HCT-116) is mediated by PI3K/Akt and mitochondrial-associated apoptotic pathway

Cited by 72 publications

References 28 publications

Ethanol extract of Ophiorrhiza pumila suppresses liver cancer cell proliferation and migration

Ethanol extract of Ophiorrhiza pumila suppresses liver cancer cell proliferation and migration

Coptisine from Coptis chinensis exerts diverse beneficial properties: A concise review

Antimalarial activity of traditional Kampo medicine Coptis Rhizome extract and its major active compounds

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