Coptis chinensis Franch polysaccharides provide a dynamically regulation on intestinal microenvironment, based on the intestinal flora and mucosal immunity

Chen, Qingqing; Ren, Rongrong; Zhang, Qingqing; Wu, Jingjing; Zhang, Yufeng; Xue, Ming-song; Yin, Dengke; Yang, Ye

doi:10.1016/j.jep.2020.113542

Cited by 45 publications

(21 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, a study demonstrated that CC has a therapeutic effect against inflammatory bowel disease through inhibiting oxidative stress, protecting intestinal epithelium's barrier, alleviating pain, regulating T helper cells, and antimicrobial activity [ 56 ]. In addition, polysaccharides in CC can regulate immune responses related to the intestinal epithelium and intestinal microenvironment in an effective, dynamic, and dose-independent manner [ 57 ]. Moreover, epiberberine in CC is considered a potential drug for treating MKN-45-associated gastric cancer, targeting the p53-independent mitochondrial pathway [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Characterizing the Antitumor Effect of Coptis chinensis and Mume Fructus against Colorectal Cancer Based on Pharmacological Analysis

Gao

2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Colorectal cancer (CRC) is the third most diagnosed cancer worldwide and is a significant cause of cancer-related deaths. Previous studies have observed that Coptis chinensis (CC) and Mume Fructus (MF) are effective against CRC, enteritis, and intestinal dysbiosis, but the chemical and pharmacological mechanisms remain poorly understood. In this study, we employed pharmacological network analysis to reveal mechanisms underlying the therapeutic effect of CC and MF against CRC. All compounds and targeted genes were obtained from the traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP). Differentially expressed genes (DEGs) were identified based on GSE146587, GSE156720, and GSE184093 datasets. A protein-protein interaction (PPI) network was constructed to identify putative target genes of CC and MF. Ten key targeted genes were identified, including CCND1, ICAM1, IL1B, IL-6, MMP1, MMP3, MMP9, MYC, SERPINE1, and VEGFA. Among these genes, six (ICAM1, IL1B, IL-6, MMP1, MMP3, MMP9, and SERPINE1) were positively correlated with levels of effector memory CD4 T cells and natural killer T cells, and three (CCND1, MYC, and VEGFA) were negatively correlated with type 17 T helper cells and CD56dim natural killer cells. Molecular docking analysis showed that four compounds of CC and MF (kaempferol, oleanolic acid, quercetin, and ursolic acid) could affect CRC by interacting with target genes. Our study proved that pharmacological analysis could reliably assess the mechanism of traditional Chinese medicines for treating cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterizing the Antitumor Effect of Coptis chinensis and Mume Fructus against Colorectal Cancer Based on Pharmacological Analysis

Gao

2022

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…Chen et al found that food polysaccharide could reduce intestinal damage in immunosuppressed mice, regulate the composition of intestinal microorganisms, increase the levels of SCFAs, and exert intestinal immunomodulatory effects (24). Coptis chinensis Franch polysaccharides, when utilized by the intestinal flora, dynamically regulates the diversity, composition and distribution of the intestinal flora and has a regulatory effect on the intestinal immune microenvironment (25). The use of proteomic techniques and flora analysis will help to explore the mechanism of action of polysaccharide immunomodulation.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics

et al. 2022

View full text Add to dashboard Cite

Proteomics and intestinal flora were used to determine the mechanism of immune modulatory effects of Flammulina velutipes polysaccharide on immunosuppressed mice. The results showed that compared with the model group, F. velutipes polysaccharide could increase thymus and spleen indices and improve thymus tissue structure in mice; IL-2 and IL-4 contents were significantly increased and IL-6 and TNF-α contents were significantly decreased; serum acid phosphatase (ACP), lactate dehydrogenase (LDH) and total antioxidant capacity (T-AOC) activities were increased (P < 0.05); in the liver, superoxide dismutase (SOD) and catalase (CAT) activities were increased (P < 0.001), while malondialdehyde (MDA) content was decreased (P < 0.001). Proteomics discovered that F. velutipes polysaccharides may exert immune modulatory effects by participating in signaling pathways such as immune diseases, transport and catabolism, phagosomes and influenza A, regulating the immune-related proteins Transferrin receptor protein 1 (TFRC) and Radical S-adenosyl methionine domain-containing protein 2 (RSAD2), etc. Gut microbial studies showed that F. velutipes polysaccharides could increase the abundance of intestinal flora and improve the flora structure. Compared to the model group, the content of short-chain fatty acids (SCFAs) and the relative abundance of SCFA-producers Bacteroides and Alloprevotella were increased in the F. velutipes polysaccharide administration group, while Lachnospiraceae_NK4A136_group and f_Lachnospiraceae_Unclassified decreased in relative abundance. Thus, F. velutipes polysaccharide may play an immunomodulatory role by regulating the intestinal environment and improving the balance of flora.

show abstract

“…The target interaction files were imported into Cytoscape 3.7.2 software for visualization and analysis. The key targets obtained above were entered into the DAVID 6.8 database, 8 and the species was limited to "Homo sapiens." Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed at P ≤ 0.05 and FDR ≤ 0.01 to screen for key pathways associated with the disease.…”

Section: Network Pharmacology and Molecular Dockingmentioning

confidence: 99%

“…has proposed the concept of "Quality markers" (Q markers) since 2016 (4,5), and many researchers have subsequently used it as an entry point for research (6). It is well-known that some components of TCM are more active and are the main medicinal components, for example, artemisinin in Artemisia annua L., berberine in Coptis chinensis Franch., baicalin in Scutellaria baicalensis Georgi, and so on (7)(8)(9). However, in many existing studies, some of them only focus on the chemical components in TCM, but ignore the active components that represent the medicinal effects.…”

Section: Introductionmentioning

confidence: 99%

Bioactive-Chemical Quality Markers Revealed: An Integrated Strategy for Quality Control of Chicory

Lin

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

As a miraculous Xinjiang Uyghur customary traditional Chinese medicine (TCM), Chicory (Cichorium glandulosum Boiss.et Huet and Cichorium intybus L.) has been found to have therapeutic potential for metabolic diseases in recent years. Although it is widely used as an ethnic medicine, there is still a lack of targeted quality control indicators in quality standards. Hence, this study was conducted to further develop a strategy to reveal bioactive-chemical quality markers based on the existing foundation. First, through the comparative screening of fingerprint profiles of a large amount of Cichorium glandulosum Boiss.et Huet and Cichorium intybus L., superiority components were found to be potential indicators of chemical quantitative properties for the roots and above-ground parts. The results of content determination showed that their contents differed among different species and parts. Second, the potential dominant components were further confirmed using network pharmacology and molecular docking techniques. Again, the results of RAW264.7 cells and L02 cells experiments showed that chicory acid and lactucin were the main components that could reflect the anti-inflammatory and uric acid-lowering potential of chicory. Finally, under this strategy, this study reveals that cichoric acid and lactucin have the properties of quality markers and quality control of chicory. In a word, this work contributes to the quality control, standard improvement, and rational clinical use of chicory.

show abstract

Coptis chinensis Franch polysaccharides provide a dynamically regulation on intestinal microenvironment, based on the intestinal flora and mucosal immunity

Cited by 45 publications

References 35 publications

Characterizing the Antitumor Effect of Coptis chinensis and Mume Fructus against Colorectal Cancer Based on Pharmacological Analysis

Characterizing the Antitumor Effect of Coptis chinensis and Mume Fructus against Colorectal Cancer Based on Pharmacological Analysis

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics

Bioactive-Chemical Quality Markers Revealed: An Integrated Strategy for Quality Control of Chicory

Contact Info

Product

Resources

About