Abstract:This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Eicosapentaenoic acid (EPA) and fucoxanthin, a carotenoid, provide a broad variety of health benefits in human nutrition. In this study, an up-and downstream process for the coproduction of EPA and fucoxanthin using the diatom Phaeodactylum tricornutum in flat-panel airlift photobioreactors is proposed. The appr… Show more
“…[1,2] Recent advances in bioprocess technology support the development of microalgal cell factories for establishing environmentally sustainable manufacturing of HVRs. In this regard, Phaeodactylum tricornutum, a unicellular, marine pennate diatom, is considered a potential feedstock for the production of biofuel and HVRs such as eicosapentaenoic acid (EPA), [3,4] docosahexaenoic acid (DHA), [5] chrysolaminarin, [6] fucoxanthin, [7,8] etc. and can be considered as a suitable microalgal cell factory for sustainable biorefinery processes.…”
Section: Introductionmentioning
confidence: 99%
“…[22] Additionally, fucoxanthin (1%-6% DCW), an important HVR, is the primary carotenoid produced in P. tricornutum. [7,23] During photosynthesis, xanthophylls act as a light-harvesting pigment connected to fucoxanthin-chlorophyll a/c-proteins (FCP), which are an integral part of the thylakoids. [24] Furthermore, due to its unique structure, fucoxanthin has various major bioactivities such as anti-oxidant, antiobesity, and anti-cancer properties, and it is an effective treatment for chronic disorders such as Alzheimer's.…”
Phaeodactylum tricornutum is a marine diatom, rich in omega‐3 polyunsaturated fatty acids especially eicosapentaenoic acid (EPA) and brown pigment, that is, fucoxanthin. These high‐value renewables (HVRs) have a high commercial and nutritional relevance. In this study, our focus was to enhance the productivities of such renewables by employing media engineering strategy via., photoautotrophic (P1, P2, P3) and mixotrophic (M1, M2, M3, M4) modes of cultivation with varying substrate combinations of carbon (glycerol: 0.1 m) and nitrogen (urea: 441 mm and/or sodium nitrate: 882 mm). Our results demonstrate that mixotrophic [M4] condition supplemented with glycerol (0.1 m) and urea (441 mm) feed enhanced productivities (mg L−1 day−1) as follows: biomass (770.0), total proteins (36.0), total lipids (22.0), total carbohydrates (23.0) with fatty acid methyl esters (9.6), EPA (2.7), and fucoxanthin (1.1), respectively. The overall yield of EPA represents 28% of total fatty acids in the mixotrophic [M4] condition. In conclusion, our improved strategy of feeding urea to a glycerol‐supplemented medium defines a new efficient biomass valorization paradigm with cost‐effective substrates for the production of HVRs in oleaginous diatoms P. tricornutum.
“…[1,2] Recent advances in bioprocess technology support the development of microalgal cell factories for establishing environmentally sustainable manufacturing of HVRs. In this regard, Phaeodactylum tricornutum, a unicellular, marine pennate diatom, is considered a potential feedstock for the production of biofuel and HVRs such as eicosapentaenoic acid (EPA), [3,4] docosahexaenoic acid (DHA), [5] chrysolaminarin, [6] fucoxanthin, [7,8] etc. and can be considered as a suitable microalgal cell factory for sustainable biorefinery processes.…”
Section: Introductionmentioning
confidence: 99%
“…[22] Additionally, fucoxanthin (1%-6% DCW), an important HVR, is the primary carotenoid produced in P. tricornutum. [7,23] During photosynthesis, xanthophylls act as a light-harvesting pigment connected to fucoxanthin-chlorophyll a/c-proteins (FCP), which are an integral part of the thylakoids. [24] Furthermore, due to its unique structure, fucoxanthin has various major bioactivities such as anti-oxidant, antiobesity, and anti-cancer properties, and it is an effective treatment for chronic disorders such as Alzheimer's.…”
Phaeodactylum tricornutum is a marine diatom, rich in omega‐3 polyunsaturated fatty acids especially eicosapentaenoic acid (EPA) and brown pigment, that is, fucoxanthin. These high‐value renewables (HVRs) have a high commercial and nutritional relevance. In this study, our focus was to enhance the productivities of such renewables by employing media engineering strategy via., photoautotrophic (P1, P2, P3) and mixotrophic (M1, M2, M3, M4) modes of cultivation with varying substrate combinations of carbon (glycerol: 0.1 m) and nitrogen (urea: 441 mm and/or sodium nitrate: 882 mm). Our results demonstrate that mixotrophic [M4] condition supplemented with glycerol (0.1 m) and urea (441 mm) feed enhanced productivities (mg L−1 day−1) as follows: biomass (770.0), total proteins (36.0), total lipids (22.0), total carbohydrates (23.0) with fatty acid methyl esters (9.6), EPA (2.7), and fucoxanthin (1.1), respectively. The overall yield of EPA represents 28% of total fatty acids in the mixotrophic [M4] condition. In conclusion, our improved strategy of feeding urea to a glycerol‐supplemented medium defines a new efficient biomass valorization paradigm with cost‐effective substrates for the production of HVRs in oleaginous diatoms P. tricornutum.
“…Recent advances in bioprocess technology supports the development of microalgal cell factories for establishing environmentally sustainable manufacturing of HVRs. In this regard Phaeodactylum tricornutum , a unicellular, marine pennate diatom, is considered as a potential feedstock for the production of biofuel and HVRs such as [2,3] , eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) [4] , chrysolaminarin [5] , fucoxanthin [6,7] , etc., and can be considered as a suitable microalgal cell factory for sustainable biorefinery processes [8] .…”
Section: Introductionmentioning
confidence: 99%
“…Additionally, fucoxanthin (1% -6% DCW) an important HVR, is the primary carotenoid produced in P.tricornutum [16] [6] . During photosynthesis, xanthophylls acts as a light harvesting pigment connected to fucoxanthin-chlorophyll a/c-proteins (FCP), which are an integral part of the thylakoids [17] .…”
Phaeodactylum tricornutum is a marine diatom, and well-studied model of
unicellular microalga. This diatom contains a wide range of high-value
renewables (HVRs) with high commercial relevance owing to their
importance in human nutrition and health. In this study, we screened P.
tricornutum for biomass, eicosapentaenoic acid (EPA) and fucoxanthin
production under photoautotrophic and mixotrophic condition with various
substrate combinations. Results highlights that culture supplemented
with glycerol and urea lead to enhanced biomass, biochemical and HVR
production. Further continuous feeding of urea in glycerol supplemented
medium results in an increase in biomass yield (0.77 g L-1) by
~ 2-fold. Additionally, continuous feeding of urea
channelizes the carbon flux towards biosynthesis of fatty acids
increasing FAME content by ~2-fold as compared to the
control conditions. Overall EPA and fucoxanthin production was 27 mg L-1
and 11 mg L-1 (~2 & 4 fold) in urea fed cultures
respectively. Present study demonstrates efficient valorization of
cost-effective substrates such as glycerol and urea for the production
of high-value renewables in P. tricornutum.
“…The dry biomass from either Chlorella and Arthrospira is used as food supplements due to the high content of proteins rich in essential amino acids (Sidari and Tofalo 2019). The biomass of Nannochloropsis species, Isochrysis species, Nitzschia species, Phaeodactylum species, and Porphyridium cruentum, is rich in ω-3 fatty acids such as eicosapentaenoic (EPA) and docosahexaenoic (DHA), which exert beneficial effects on human health (Sidari and Tofalo 2019;Jiménez Callejón et al 2020;Derwenskus et al 2020).…”
The demand for natural antioxidants to be used in food industry is increasing, as synthetic antioxidants are toxic and have high production costs. Specifically, food processing and preservation require antioxidants resistant to thermal sterilization processes. In this study, twenty-five strains among microalgae and cyanobacteria were screened as antioxidants producers. The species Enallax sp., Synechococcus bigranulatus and Galdieria sulphuraria showed the highest content of chlorophyll a and total carotenoids. In vitro stability and antioxidant activity of the ethanolic extracts were performed. The results revealed that pigments present in the extracts, obtained from the previously mentioned species, were stable at room temperature and exhibited in vitro free radical scavenging potential with IC 50 values of 0.099 ± 0.001, 0.048 ± 0.001 and 0.13 ± 0.02 mg mL -1 , respectively. Biocompatibility assay showed that the extracts were not toxic on immortalized cell lines. The antioxidant activity was also tested on a cell-based model by measuring intracellular ROS levels after sodium arsenite treatment. Noteworthy, extracts were able to exert the same protective effect, before and after the pasteurization process. Results clearly indicate the feasibility of obtaining biologically active and thermostable antioxidants from microalgae. Green solvents can be used to obtain thermo-resistant antioxidants from cyanobacteria and microalgae which can be used in the food industry. Thus, the substitution of synthetic pigments with natural ones is now practicable.
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