Coproduction of Enzymes and Beta-Glucan by Aspergillus oryzae Using Solid-State Fermentation of Brown Rice

Abstract

The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698 was investigated. Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation. To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid. Pretreatment of brown rice for 60 min prior to inoculation enhanced fungal biomass, while increasing the production of enzymes and β-glucan using solidstate fermentation. Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation. Therefore, solidstate fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.

Evaluating comparative β-glucan production aptitude of Saccharomyces cerevisiae, Aspergillus oryzae, Xanthomonas campestris, and Bacillus natto

“…A. niger can produce a yield of 9–11% ( Paulraj et al, 2012 ). In a recent approach, a maximum fungal biomass of 0.76 mg/g with β-glucan at 9.34% (w/w) was obtained during solid-state fermentation of brown rice by A. oryzae KCCM 12698 ( Ji and Ra, 2021 ).…”

Changes in bioactive compounds during solid‐state fermentation of Sphenarium purpurascens with Aspergillus oryzae

“…β‐glucan reached 2.4% w/w on day 4 of the fermentation (8.4 pH). In a study of brown rice fermented with A. oryzae , the maximum value of β‐glucan was 7.09% w/w at 7 pH 7 (Ji & Chae Hun, 2021). It is suggested to control pH and have a source of carbohydrates to obtain higher among β‐glucans (Bai et al ., 2021).…”

Comparison of Liquid and Solid-State Fermentation Processes for the Production of Enzymes and Beta-Glucan from Hulled Barley

“…Aspergillus oryzae NIBRFGC000501965 was obtained from the National Institute of Biological Resources (NIBR, Korea). The culture was carried out as described in our previous reports [ 5 ]. Briefly, the spore suspension (1×10 8 spores/ml) was inoculated in a 250-ml Erlenmeyer flask containing 100 ml of the modified synthetic medium comprising 70 g/l hulled barley powder, 5 g/l soytone, and 6 g/l ascorbic acid (Vitamin C).…”

“…The activities of amylase and protease were determined according to the procedures described in previous reports [ 5 , 13 ]. Amylase activity was carried out by measuring the reducing sugars released from soluble starch.…”

“…The advantages of SSF are high economic viability, use of cheap substrates, low production costs, high enzyme yields, and less energy consumption [ 4 ]. According to our previous study [ 5 ], SSF of brown rice showed significant potential for improving enzyme and β-glucan production. Thus, in this study, SSF using hulled barley was carried out to monitor its effects on enzyme and β-glucan production.…”