Copro-PCR based detection of Schistosoma eggs using mitochondrial DNA markers

Gobert, Geoffrey N.; Chai, M.; Duke, Mary; McManus, Donald P.

doi:10.1016/j.mcp.2005.01.006

Cited by 52 publications

(30 citation statements)

References 13 publications

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“…Failure in this one case could be because some people do not swallow sputum. Similar methods to detect copro-DNA have been developed for schistosomiasis (Gobert et al, 2005) and cestode infections (Yamasaki et al, 2004;Mathis and Deplazes, 2006). Intapan and others (2005) have demonstrated that the approach is effective in diagnosing P. heterotremus in cats.…”

Section: Discussionmentioning

confidence: 99%

Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations

Nkouawa

Okamoto

Mabou³

et al. 2009

Transactions of the Royal Society of Tropical Medicine and Hygi

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SummaryParagonimiasis is a common parasitic zoonosis in Cameroon and neighboring countries in Western Africa. Serum, sputum and faecal samples were collected in an endemic area of South West Province, Cameroon, after administration of a questionnaire to identify individuals with appropriate symptoms and histories. Microscopic examination revealed eggs in sputum from 16 people, but none in any faecal sample. These 16 were among the 25 and 26 people respectively positive by enzyme-linked immunosorbent assay (ELISA) and by immunoblot using Paragonimus africanus crude antigens. The detection of copro-DNA by polymerase chain reaction (PCR) was attempted using 23 faecal samples (18 from sputum egg-negative and 5 from sputum egg-positive individuals). The copro-DNA was detected in 4 of the 5 sputum eggpositive individuals. These results suggest that (1) serology is much more sensitive than sputum examination for diagnosis of paragonimiasis, and (2) a copro-DNA test may be more sensitive than a microscopic search for eggs in faeces. Molecular sequence data from ITS2 and cox1 genes confirmed that adult worms experimentally raised in cats were P. africanus and that eggs from sputum or other worm products from human faeces also belonged to this species. Based on these results, 26 of 168 persons (15.5%) were diagnosed as suffering from paragonimiasis.

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Section: Discussionmentioning

confidence: 99%

Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations

Nkouawa

Okamoto

Mabou³

et al. 2009

Transactions of the Royal Society of Tropical Medicine and Hygi

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“…In patients with acute disease (Katayama syndrome), both serology and direct detection may produce false-negative results. As a consequence, several groups have developed specific and highly sensitive PCR-based assays for the detection of S. japonicum DNA in feces or serum/plasma specimens of infected animals, including mice, rabbits, and pigs, which potentially provide a test for early diagnosis and therapy evaluation in humans (74,123,125,214). In a recent development, a PCR test for detection of cell-free parasite DNA (CFPD) in human plasma has been devised, which may provide a new laboratory tool for diagnosing schistosomiasis in all phases of clinical disease, including the capacity to rule out Katayama syndrome and active disease (201).…”

Section: Pcr Methodsmentioning

confidence: 99%

Schistosomiasis in the People's Republic of China: the Era of the Three Gorges Dam

et al. 2010

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SUMMARY The potential impact of the Three Gorges Dam (TGD) on schistosomiasis transmission in China has invoked considerable global concern. The TGD will result in changes in the water level and silt deposition downstream, favoring the reproduction of Oncomelania snails. Combined with blockages of the Yangtze River's tributaries, these changes will increase the schistosomiasis transmission season within the marshlands along the middle and lower reaches of the Yangtze River. The changing schistosome transmission dynamics necessitate a comprehensive strategy to control schistosomiasis. This review discusses aspects of the epidemiology and transmission of Schistosoma japonicum in China and considers the pathology, clinical outcomes, diagnosis, treatment, immunobiology, and genetics of schistosomiasis japonica together with an overview of current progress in vaccine development, all of which will have an impact on future control efforts. The use of synchronous praziquantel (PZQ) chemotherapy for humans and domestic animals is only temporarily effective, as schistosome reinfection occurs rapidly. Drug delivery requires a substantial infrastructure to regularly cover all parts of an area of endemicity. This makes chemotherapy expensive and, as compliance is often low, a less than satisfactory control option. There is increasing disquiet about the possibility that PZQ-resistant schistosomes will develop. Consequently, as mathematical modeling predicts, vaccine strategies represent an essential component in the future control of schistosomiasis in China. With the inclusion of focal mollusciciding, improvements in sanitation, and health education into the control scenario, China's target of reducing the level of schistosome infection to less than 1% by 2015 may be achievable.

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“…Sensitive detection of S. haematobium-specific DNA in urine samples has been successful with urine sediments on filter papers (205). In addition to the detection of nuclear DNA, mitochondrial gene segment amplification has been shown to provide both a high level of sensitivity, due to the availability of numerous copies within a single cell, and pronounced species specificity (55,206), a feature important in differentiating between different schistosome species (197,202). Improvements in conventional PCR in combination with other techniques, such as restriction fragment length polymorphism (PCR-RFLP) analysis (207) and PCR-ELISA (208), have also been applied for the detection of schistosome infections.…”

Section: Detection Of Schistosoma Dna By Pcrmentioning

confidence: 99%

“…The technique has been used for the identification of the different human schistosome species and in assessing infection intensity (197,209,210). An expansion on qPCR techniques is multiplex PCR, which amplifies more than one DNA target in a single reaction mixture and has been used successfully for case detection (197), for differentiating between S. japonicum, S. mansoni, and S. haematobium, and as an important tool in epidemiological studies and in monitoring schistosomiasis control programs (197,206). In a recent advance in PCR technology, droplet digital PCR (dd PCR) was developed and has proven to be more sensitive and precise than qPCR (211)(212)(213).…”

Section: Detection Of Schistosoma Dna By Pcrmentioning

confidence: 99%

Advances in the Diagnosis of Human Schistosomiasis

et al. 2015

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SUMMARY Schistosomiasis is a major neglected tropical disease that afflicts more than 240 million people, including many children and young adults, in the tropics and subtropics. The disease is characterized by chronic infections with significant residual morbidity and is of considerable public health importance, with substantial socioeconomic impacts on impoverished communities. Morbidity reduction and eventual elimination through integrated intervention measures are the focuses of current schistosomiasis control programs. Precise diagnosis of schistosome infections, in both mammalian and snail intermediate hosts, will play a pivotal role in achieving these goals. Nevertheless, despite extensive efforts over several decades, the search for sensitive and specific diagnostics for schistosomiasis is ongoing. Here we review the area, paying attention to earlier approaches but emphasizing recent developments in the search for new diagnostics for schistosomiasis with practical applications in the research laboratory, the clinic, and the field. Careful and rigorous validation of these assays and their cost-effectiveness will be needed, however, prior to their adoption in support of policy decisions for national public health programs aimed at the control and elimination of schistosomiasis.

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Copro-PCR based detection of Schistosoma eggs using mitochondrial DNA markers

Cited by 52 publications

References 13 publications

Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations

Paragonimiasis in Cameroon: molecular identification, serodiagnosis and clinical manifestations

Schistosomiasis in the People's Republic of China: the Era of the Three Gorges Dam

Advances in the Diagnosis of Human Schistosomiasis

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