Copro-molecular identification of intestinal nematode infections in a rural community in East Malaysia

Hassan, Nur-Amirah; Thodsapol, Autchariya; Lim, Yvonne Ai Lian; Sulaiman, Wan Yusoff Wan; Omar, Siti Farah Norasyikeen Sidi; Umsakul, Kamontam; Ngui, Romano

doi:10.1016/j.parint.2020.102237

Cited by 2 publications

(7 citation statements)

References 37 publications

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“…We detected six different taxonomic units of parasites in the examined stool samples in the current report. The most common parasitic eggs in the samples were A. lumbricoides in 13.8%, which corresponds to the results of Safi et al [26] in Afghanistan and Hassan et al in Malaysia [27]. Protozoal cysts G. duodenalis, which are the most common cause of diarrheal disease in some countries [28], were detected microscopically in 6.3% of stool samples we examined.…”

Section: Discussionsupporting

confidence: 88%

“…In Nicaragua, the prevalence of parasitic infections in children was up to 54.3% [38]. A higher prevalence of parasitic infections was reported in Malaysia, where parasitic infections were found in 56.3% of examined children [27]. The most common finding in 28.4% of Roma children was the roundworm eggs, which corresponds to Rudohradská et al [25], who diagnosed roundworm eggs in 24.7% of Roma children.…”

Section: Discussionmentioning

confidence: 87%

“…The most common finding in 28.4% of Roma children was the roundworm eggs, which corresponds to Rudohradská et al [25], who diagnosed roundworm eggs in 24.7% of Roma children. Similarly, Hassan et al [27] diagnosed A. lumbricoides in 24.3% of children in Malaysia. We confirmed a high 13.8% prevalence of G. duodenalis cysts.…”

Section: Discussionmentioning

confidence: 90%

“…Safi et al [26] reached similar findings in a survey on stool samples from Afghan compulsory school children, which revealed the presence of parasitic developmental stages in a 26.6% prevalence. A much higher prevalence was found, e.g., in Malaysia, where up to 56.3% of examined children under the age of 18 were positive for parasitic infections [27].…”

Section: Discussionmentioning

confidence: 96%

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Neglected Diseases—Parasitic Infections among Slovakian Children from Different Populations and Genotypes of Giardia duodenalis

et al. 2022

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Children are most prone to parasitic infections. The objectives of the study were to examine the occurrence of parasitic infections in children from different populations and to perform molecular characterization of human Giardia duodenalis isolates. We examined 631 stool samples from Roma and non-Roma children for the presence of parasitic developmental stages. Samples were collected from three eastern Slovakia districts. The ages of the children ranged from 1 months to 17 years. Subsequently, the molecular characterization of human G. duodenalis isolates by PCR detected triosephosphate isomerase (tpi) and beta-giardin (bg) genes was performed. The overall prevalence of parasitic infection was 19.8%. Ascaris lumbricoides eggs were the most frequent, with an occurrence of about 13.8%. G. duodenalis cysts were present in 6.3% of samples. G. duodenalis isolates obtained from 13 children were subjected to DNA sequencing with tpi and bg genes. Five isolates were categorized as bearing subassemblage BIII, the three isolates as subassemblage BIV, one person was infected with a mixture of subassemblages BIII and BIV, four children had subassemblage AII, and one isolate revealed a structure corresponding with subassemblage AI. Our work is proof that poverty and poor hygiene contribute the most to public health problems associated with neglected parasitic diseases.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 96%

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Neglected Diseases—Parasitic Infections among Slovakian Children from Different Populations and Genotypes of Giardia duodenalis

et al. 2022

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“…Well-defined positive controls of A. lumbricoides, T. trichiura, N. americanus, A. duodenale, and A. lumbricoides were obtained from the unused and archived specimens from our previous studies (Rajoo et al, 2017;Mohd-Shaharuddin et al, 2018;Hassan et al, 2021). These samples were collected from the indigenous communities.…”

Section: Control Samples Preparationmentioning

confidence: 99%

A conventional multiplex PCR for the detection of four common soil-transmitted nematodes in human feces: development and validation

N.A.¹

2022

Trop Biomed

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Soil-transmitted helminth (STH) infections, mainly caused by Ascaris lumbricoides, Trichuris trichiura, and hookworms, are among the most common intestinal parasites that infect humans. The infections are widely distributed throughout tropical and subtropical countries, including Malaysia, particularly in underprivileged communities. Microscopic and culture techniques have been used as a gold standard for diagnostic techniques. However, these methods yield low sensitivity and specificity, laborious and time-consuming. Therefore, simple, rapid, and accurate alternative methods are needed for the simultaneous detection of STH infections. Although advanced technologies such as real-time multiplex PCR have been established, the use of this technique as a routine diagnostic is limited due to the high cost of the instrument. Therefore, a single-round multiplex conventional PCR assay for rapid detection of four STH species in the fecal sample was developed in this study. To perform the single-round multiplex PCR, each pair of species-specific primers was selected from target genes, including Ancylostoma duodenale (Internal Transcribed Spacer 2; accession No. AJ001594; 156 base pair), Necator americanus (ITS 2; accession No. AJ001599; 225 base pair), Ascaris lumbricoides (Internal Transcribed Spacer 1; accession No. AJ000895; 334 base pair) and Trichuris triciura (partial ITS 1, 5.8s rRNA and partial ITS 2; accession No. AM992981; 518 base pair). The results showed that the newly designed primers could detect the DNA of STH at low concentrations (0.001 ng/μl) with no cross-amplification with other species. This assay enables the differentiation of single infections as well as mixed infections. It could be used as an alternative and is a convenient method for the detection of STHs, especially for the differentiation of N. americanus and A. duodenale.

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Copro-molecular identification of intestinal nematode infections in a rural community in East Malaysia

Cited by 2 publications

References 37 publications

Neglected Diseases—Parasitic Infections among Slovakian Children from Different Populations and Genotypes of Giardia duodenalis

Neglected Diseases—Parasitic Infections among Slovakian Children from Different Populations and Genotypes of Giardia duodenalis

A conventional multiplex PCR for the detection of four common soil-transmitted nematodes in human feces: development and validation

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