Coprinus cinereus DNA ligase I during meiotic development

Namekawa, Satoshi H.; Hamada, Fumika N.; Ishii, S; Ichijima, Yosuke; Yamaguchi, Taiki; Nara, Takayuki; Kimura, Seisuke; Ishizaki, Takashi; Iwabata, Kazuki; Koshiyama, Akiyo; Teraoka, Hirobumi; Sakaguchi, Kengo

doi:10.1016/s0167-4781(03)00073-3

Cited by 9 publications

(8 citation statements)

References 48 publications

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“…Signals for CcFEN-1 were thus abundant in nuclei of meiotic cells throughout meiotic cell cycle. These results were coincided with the data in our previous reports (Hamada et al, 2002, Namekawa et al, 2003a. The levels of the other DNA replication related proteins such as PCNA, DNA polymerase a, DNA primase, and DNA ligase I (LIG1) appear to be unchanged through the meiotic cell cycle, while the mRNA levels were remarkably changed.…”

Section: Meiotic Expression Of Ccfen-1supporting

confidence: 91%

“…CcPCNA and CcLIG1 transcripts are also reported to be expressed mostly in leptotene and zygotene, as with CcFEN-1, and CcPCNA binds to CcLIG1 during meiotic prophase I (Hamada et al, 2002;Namekawa et al, 2003a). FEN-1 is known to work with PCNA and DNA ligase I in Okazaki fragment processing and long patch base excision repair (Levin et al, 2000;Maga et al, 2001).…”

Section: Meiotic Expression Of Ccfen-1mentioning

confidence: 99%

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Expression of flap endonuclease-1 during meiosis in a basidiomycete, Coprinus cinereus

Yamaguchi

Namekawa

Hamada

et al. 2004

Fungal Genetics and Biology

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Section: Meiotic Expression Of Ccfen-1supporting

confidence: 91%

Section: Meiotic Expression Of Ccfen-1mentioning

confidence: 99%

Expression of flap endonuclease-1 during meiosis in a basidiomycete, Coprinus cinereus

Yamaguchi

Namekawa

Hamada

et al. 2004

Fungal Genetics and Biology

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“…The ink-cap mushroom, Coprinopsis cinerea (formerly Coprinus cinereus), is a well-studied homobasidiomycete (12,43,47) that forms an excellent model system for studies of gene expression at several levels of differentiation, particularly mushroom development and meiotic processes (46,59). It has been used as an object for studies of development (32), mainly because of its relatively short life cycle, which can be completed in the laboratory within 2 weeks (44).…”

mentioning

confidence: 99%

Characterization of Serine Proteinase Expression in Agaricus bisporus and Coprinopsis cinerea by Using Green Fluorescent Protein and the A. bisporus SPR1 Promoter

Heneghan

Porta

Zhang

et al. 2009

Appl Environ Microbiol

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The Agaricus bisporus serine proteinase 1 (SPR1) appears to be significant in both mycelial nutrition and senescence of the fruiting body. We report on the construction of an SPR promoter::green fluorescent protein (GFP) fusion cassette, pGreen_hph1_SPR_GFP, for the investigation of temporal and developmental expression of SPR1 in homobasidiomycetes and to determine how expression is linked to physiological and environmental stimuli. Monitoring of A. bisporus pGreen_hph1_SPR_GFP transformants on media rich in ammonia or containing different nitrogen sources demonstrated that SPR1 is produced in response to available nitrogen. In A. bisporus fruiting bodies, GFP activity was localized to the stipe of postharvest senescing sporophores. pGreen_hph1_SPR_GFP was also transformed into the model basidiomycete Coprinopsis cinerea. Endogenous C. cinerea proteinase activity was profiled during liquid culture and fruiting body development. Maximum activity was observed in the mature cap, while activity dropped during autolysis. Analysis of the C. cinerea genome revealed seven genes showing significant homology to the A. bisporus SPR1 and SPR2 genes. These genes contain the aspartic acid, histidine, and serine residues common to serine proteinases. Analysis of the promoter regions revealed at least one CreA and several AreA regulatory motifs in all sequences. Fruiting was induced in C. cinerea dikaryons, and fluorescence was determined in different developmental stages. GFP expression was observed throughout the life cycle, demonstrating that serine proteinase can be active in all stages of C. cinerea fruiting body development. Serine proteinase expression (GFP fluorescence) was most concentrated during development of young tissue, which may be indicative of high protein turnover during cell differentiation.

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“…We found that transcripts of the 3R enzymes as described below are abundant at meiotic prophase I and we have previously discussed the roles of the 3R enzymes during meiosis. The enzymes are PCNA (CcPCNA) [63], DNA ligase I [64], DNA ligase IV [65], Flap endonuclease‐1 [66], Lim15/Dmc1 (CcLim15) [67], Rad51 (CcRad51) [68,69] and DNA topoisomerase II (CcTopII) [31].…”

Section: Biomaterials For Meiotic Studiesmentioning

confidence: 99%

“…By taking advantage of the properties of this organism as described above, we succeeded in establishing cDNA libraries from mRNAs from C. cinereus meiotic cells at leptotene, zygotene and pachytene and have studied 3R (DNA replication, repair, recombination) enzymes from each stage [31, [63][64][65][66][67][68][69]. We found that transcripts of the 3R enzymes as described below are abundant at meiotic prophase I and we have previously discussed the roles of the 3R enzymes during meiosis.…”

Section: Biomaterials For Meiotic Studiesmentioning

confidence: 99%

Meiosis and small ubiquitin‐related modifier (SUMO)‐conjugating enzyme, Ubc9

2007

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In this review, we describe the role of a small ubiquitin‐like protein modifier (SUMO)‐conjugating protein, Ubc9, in synaptonemal complex formation during meiosis in a basidiomycete, Coprinus cinereus. Because its meiotic cell cycle is long and naturally synchronous, it is suitable for molecular biological, biochemical and genetic studies of meiotic prophase events. In yeast two‐hybrid screening using the meiotic‐specific cDNA library of C. cinereus, we found that the meiotic RecA homolog CcLim15 interacted with CcUbc9, CcTopII and CcPCNA. Moreover, both TopII and PCNA homologs were known as Ubc9 interactors and the targets of sumoylation. Immunocytochemistry demonstrates that CcUbc9, CcTopII and CcPCNA localize with CcLim15 in meiotic nuclei during leptotene to zygotene when synaptonemal complex is formed and when homologous chromosomes pair. We discuss the relationships between Lim15/Dmc1 (CcLim15), TopII (CcTopII), PCNA (CcPCNA) and CcUbc9, and subsequently, the role of sumoylation in the stages. We speculate that CcLim15 and CcTopII work in cohesion between homologous chromatins initially and then, in the process of the zygotene events, CcUbc9 works with factors including CcLim15 and CcTopII as an inhibitor of ubiquitin‐mediated degradation and as a metabolic switch in the meiotic prophase cell cycle. After CcLim15–CcTopII dissociation, CcLim15 remains on the zygotene DNA and recruits CcUbc9, Rad54B, CcUbc9, Swi5‐Sfr1, CcUbc9 and then CcPCNA in rotation on the C‐terminus. Finally during zygotene, CcPCNA replaces CcLim15 on the DNA and the free‐CcLim15 is probably ubiquitinated and disappears. CcPCNA may recruit the polymerase. The idea that CcUbc9 intervenes in every step by protecting CcLim15 and by switching several factors at the C‐terminus of CcLim15 is likely. At the boundary of the zygotene and pachytene stages, CcPCNA would be sumoylated. CcUbc9 may also be involved with CcPCNA in the switch from the replicative polymerase being recruited at zygotene to the repair‐type DNA polymerases being recruited at pachytene.

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Coprinus cinereus DNA ligase I during meiotic development

Cited by 9 publications

References 48 publications

Expression of flap endonuclease-1 during meiosis in a basidiomycete, Coprinus cinereus

Expression of flap endonuclease-1 during meiosis in a basidiomycete, Coprinus cinereus

Characterization of Serine Proteinase Expression in Agaricus bisporus and Coprinopsis cinerea by Using Green Fluorescent Protein and the A. bisporus SPR1 Promoter

Meiosis and small ubiquitin‐related modifier (SUMO)‐conjugating enzyme, Ubc9

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