Coprinopsis cinerea intracellular lactonases hydrolyze quorum sensing molecules of Gram-negative bacteria

Stöckli, Martina; Lin, Chi Feng; Sieber, Ramon; Plaza, David Fernando; Ohm, Robin A.; Künzler, Markus

doi:10.1016/j.fgb.2016.07.009

Cited by 17 publications

(13 citation statements)

References 74 publications

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“…Since homologous recombination is a rare event in the C. cinerea AmutBmut wt strain, a Δku70 variant of this strain, which is deficient in non‐homologous end‐joining (Nakazawa and Honda, ), was used for this purpose. The cop6 gene was replaced by the heterologous Pcpab cassette (Stöckli et al , ) (Fig. ).…”

Section: Resultsmentioning

confidence: 99%

“…The deletion of the terpene synthase cop6 gene was carried out in the C. cinerea AmutBmut Δku70 strain ku3‐24 27 in an analogous manner to that described in (Stöckli et al , ). A detailed description of the procedure can be found in the .…”

Section: Methodsmentioning

confidence: 99%

“…To monitor the expression of the terpene gene cluster under various growth conditions, RNA was extracted from the fungal mycelium and cDNA was prepared as described previously (Plaza et al , ). qRT‐PCR and data analysis was performed as described by Stöckli et al () using the primers listed in Table .…”

Section: Methodsmentioning

confidence: 99%

“…About 400 µl of this suspension was added to the bead plates. The whole cell proteins (WCPs) of the fungal mycelium were extracted as described previously (Stöckli et al , ). WCP samples were boiled in Lämmli Buffer, run on a 12% polyacrylamide gel and transferred to a nitrocellulose membrane.…”

Section: Methodsmentioning

confidence: 99%

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Bacteria‐induced production of the antibacterial sesquiterpene lagopodin B in Coprinopsis cinerea

Stöckli

Morinaka

Lackner

et al. 2019

Molecular Microbiology

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Fungi defend their ecological niche against antagonists by producing antibiosis molecules. Some of these molecules are only produced upon confrontation with the antagonist. The basidiomycete Coprinopsis cinerea induces the expression of the sesquiterpene synthase-encoding gene cop6 and its two neighboring genes coding for cytochrome P450 monooxygenases in response to bacteria. We further investigated this regulation of cop6 and examined if the gene product is involved in the production of antibacterials. Cell-free supernatants of axenic cultures of the Gram-positive bacterium Bacillus subtilis were sufficient to induce cop6 transcription assessed using a fluorescent reporter strain. Use of this strain in a microfluidic device revealed that the cop6 gene was induced in all hyphae directly exposed to the supernatant and that induction occurred within less than one hour. Targeted replacement of the cop6 gene demonstrated the requirement of the encoded synthase for the biosynthesis of the sesquiterpene lagopodin B, a previously reported antibacterial compound from related species. Accordingly, lagopodin B from C. cinerea inhibited the growth of several Gram-positive bacteria including B. subtilis but not Gram-negative bacteria. Our results demonstrate that the C. cinerea vegetative mycelium responds to soluble compounds of a bacterial culture supernatant by local production of an antibacterial secondary metabolite.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Bacteria‐induced production of the antibacterial sesquiterpene lagopodin B in Coprinopsis cinerea

Stöckli

Morinaka

Lackner

et al. 2019

Molecular Microbiology

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“…On the other hand, the presence of biofilms on the growing active area of fungal colonies may limit the capacity of fungi to degrade organic matter and induce a competition for nutrients between the bacterial community and the hyphae. In this second hypothesis, we expect that fungi, and particularly saprotrophic fungi, would have developed strategies to block the formation of biofilms on their hyphae (Stöckli et al ., 2016; Essig et al ., 2014). Our data support the hypothesis of de Carvalho and colleagues (de Carvalho et al ., 2015), in which the mycelium of ECM fungi is rather permissive to bacterial biofilm formation although exceptions like T. melanosporum exist.…”

Section: Discussion and Perspectivesmentioning

confidence: 99%

Bacterial biofilm formation on soil fungi: a widespread ability under controls

Guennoc¹,

Rose²,

Labbé³

et al. 2017

Preprint

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In natural environments, bacteria preferentially live in biofilms that they build on abiotic surfaces but also on living tissues. Although fungi form extensive networks of hyphae within soils and thus could provide immense surfaces for bacteria to build biofilms and to proliferate, the extent on such phenomenon and the consequences for the fitness of both microorganisms is poorly known in soils. Here, we analyzed the process of formation of biofilms by various bacteria on hyphae of soil fungi in an in vitro setting using confocal and electron microscopy. We showed that the ability to form biofilms on fungal hyphae is widely shared among soil bacteria. In contrast, some fungi, mainly belonging to the Ascomycete class, did not allow for the formation of bacterial biofilms on their surfaces. The formation of biofilms was also strongly modulated by the presence of tree roots and by the development of the ectomycorrhizal symbiosis, suggesting that biofilm formation does not occur randomly in soil but that it is highly regulated by several biotic factors. Finally, our study led to the unexpected finding that networks of filaments made of extracellular DNA were used to build the skeleton of biofilms by a large array of bacteria.

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Benefits of fungal-to-bacterial quorum quenching as anti-biofouling strategy in membrane bioreactors for wastewater treatment and water reuse

Park,

Jang

et al. 2024

Bioresource Technology

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Coprinopsis cinerea intracellular lactonases hydrolyze quorum sensing molecules of Gram-negative bacteria

Cited by 17 publications

References 74 publications

Bacteria‐induced production of the antibacterial sesquiterpene lagopodin B in Coprinopsis cinerea

Bacteria‐induced production of the antibacterial sesquiterpene lagopodin B in Coprinopsis cinerea

Bacterial biofilm formation on soil fungi: a widespread ability under controls

Benefits of fungal-to-bacterial quorum quenching as anti-biofouling strategy in membrane bioreactors for wastewater treatment and water reuse

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