Coprinopsis cinerea from rice husks forming sclerotia in agar culture

Watanabe, Tsuneo; Tagawa, Masahiro; Tamaki, Hideyuki; Hanada, Satoshi

doi:10.1007/s10267-010-0075-2

Cited by 4 publications

(2 citation statements)

References 11 publications

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“…Sclerotia are persistent resting structures that developed under continuous dark (14). They are multicellular structures in round shape, with internal medulla tissue and external rind tissue (15). The whole sclerotia are pigmented and in brown color (16).…”

Section: Introductionmentioning

confidence: 99%

Carbon metabolism and transcriptome in developmental paths differentiation of a homokaryotic Coprinopsis cinerea strain

Xie¹,

Chang²,

Kwan³

2020

Fungal Genetics and Biology

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The balance and interplay between sexual and asexual reproduction is one of the most attractive mysteries in fungi. The choice of developmental strategy reflects the ability of fungi to adapt to the changing environment. However, the evolution of developmental paths and the metabolic regulation during differentiation and morphogenesis are poorly understood.Here, we monitor the carbohydrate metabolism and gene expression regulation during the early differentiation process from the "fungal stem cell", vegetative mycelium, to the highly differentiated tissue/cells, fruiting body, oidia or sclerotia, of a homokaryotic fruiting Coprinopsis cinerea strain A43mut B43mut pab1-1 #326, uncovering the systematic changes during morphogenesis and the evolutionary process of developmental strategies. Conversion between glucose and glycogen and conversion between glucose and beta-glucan are the main carbon flows in the differentiation processes. Genes related to carbohydrate transport and metabolism are significantly differentially expressed among paths. RNA editing, a novel layer of gene expression regulation, occurs in all four developmental paths and enriched in ImportanceFungi is a group of species with high diversity and plays essential roles to the ecosystem. The life cycle of fungi is complex in structure and delicate in function. Choice of developmental strategies and internal changes within the organism are both important for the fungus to fulfill their ecological functions, reflecting the relationship between environment and the population. This study put the developmental process of vegetative growth, sexual and asexual reproduction, resistant structure formation of a classical model basidiomycetes fungus, C. cinerea, together for the first time to view the developmental paths differentiation process with physiology, transcriptomics and evolutionary prospects. Carbohydrate assays and RNA-seq showed the changes of the fungus. Our results fill the gaps on gene expression regulation during the early stage of developmental paths differentiation, and expand our understanding of the evolutionary process of life history and reproductive strategy in fungi.

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Section: Introductionmentioning

confidence: 99%

Carbon metabolism and transcriptome in developmental paths differentiation of a homokaryotic Coprinopsis cinerea strain

Xie¹,

Chang²,

Kwan³

2020

Fungal Genetics and Biology

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“…The sclerotia are small, oval or globular, symmetrical, multi-cellular resting bodies (about 0.1-0.5 mm in Ø) comprised of an outer uni-or multi-layered pseudoparenchymatous rind, built of compact cells (Volz and Niederpruem 1970;). The internal prosenchymatous medulla is described to be composed of large, ovulate and globular cells of chlamydospore-like appearance with non-stained thickened macrofibrillar cell walls (Volz and Niederpruem 1970;Waters et al 1972Badalyan et al 2011;Watanabe et al 2011). 1975b) studied in phase contrast microscopy the earliest morphogenetic stages of colonies of C. cinerea grown on a slide and reported that sclerotia originated from intercalary hyphal cells.…”

Section: Introductionmentioning

confidence: 99%

Fruiting body development of Coprinopsis cinerea: Cytology and regulatory factors

Subba¹

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Chapter 1. Introduction………………………………………………………………. 22 1.1.Fungi in general 1.2.History on Coprinopsis cinerea as a model organism 1.3.Life cycle of C. cinerea 1.4.Fruiting body development 1.5.Genetic and environmental regulator in the fruiting process 1.6.Self-compatible AmutBmut and other mutants 1.7.Aims of the study 1.8.Outcome of the study 1.9. References Chapter 2. Two alternative routes of development: early decisions ………………… 62 Subchapter 2.1. Pathway committed to fruiting: secondary hyphal knot formation...64 2.1.1. Abstract 2.1.2. Introduction 2.1.3. Materials and methods 2.1.3.1. Strains, media and standard cultivation conditions 2.1.3.2. Cultivation for the study of primary hyphal knot and microscopy 2.1.3.3. Cultivation for the observation of secondary hyphal knots, primordia and mature carpophores 2.1.3.4. Photography 2.1.4. Results 2.1.4.1 Primary hyphal knot formation in dark 2.1.4.2. Light induced secondary hyphal knot formation 2.1.4.3. Detail observation on Sk formation 2.1.4.4. Secondary hyphal knot formation in homokaryon AmutBmut and dikaryon PS1X2 at 25 °C under a normal day/night rhythm, observed first at 12 h after putting the plates into fruiting conditions 5 2.1.4.4.1. Observation for the homokaryon AmutBmut 2.1.4.4.2Observation for the dikaryon PS1X2 2.1.5. Discussion 2.1.5.1. The term primary and secondary hyphal knots in the literature 2.1.5.2. Techniques to obtain Pks and Sks 2.1.5.3. Origin of knots from single or multiple hyphae? 2.1.5.4. Observation on the formation of Sks in the current study 2.1.5.5. Terminology of hyphal knots 2.1.6. Conclusion 2.1.7. References Subchapter 2.2. Sclerotia formation as an alternate development pathway to fruiting in Coprinopsis cinerea …………………………………………………… 88 2.

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Primary simple assays of cellulose-degrading fungi

et al. 2012

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Some 25 fungi, including at least 14 basidiomycetes, one ascomycete, and five anamorphic fungi were evaluated for their cellulose-degrading abilities in Difco potato dextrose broth or Difco malt extract broth cultures with cellulosic substrates (e.g., filter paper) in plastic Petri dishes. Among them, Peniophora sp. 06-13 and Phlebia sp. 99-335 reduced the dry weights of the whole cultures with these substrates more than the dry weights of the respective original substrates after 30 days of culture, showing definite cellulose degradation. In the cultures with more than 10 test fungi including Pycnoporus coccineus 84-117, such weight losses did not occur. This assay technique for the primary screening for cellulose degrading fungi is simple, inexpensive, reproducible and accurate.

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Coprinopsis cinerea from rice husks forming sclerotia in agar culture

Cited by 4 publications

References 11 publications

Carbon metabolism and transcriptome in developmental paths differentiation of a homokaryotic Coprinopsis cinerea strain

Carbon metabolism and transcriptome in developmental paths differentiation of a homokaryotic Coprinopsis cinerea strain

Fruiting body development of Coprinopsis cinerea: Cytology and regulatory factors

Primary simple assays of cellulose-degrading fungi

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