Copper toxicity in gills of the teleost fish, Oreochromis niloticus: Effects in apoptosis induction and cell proliferation

Monteiro, Sandra M.; Santos, Nuno M.S. dos; Calejo, Margarida; Fontaínhas‐Fernandes, A.; Sousa, Mário

doi:10.1016/j.aquatox.2009.07.008

Cited by 81 publications

(34 citation statements)

References 40 publications

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“…DNA fragmentation is a biomarker of apoptosis in cells (Chai et al 2010). The number of TUNELpositive cells is a widely used index of DNA fragmentation (Kaptaner and Unal 2011;Monteiro et al 2009). …”

Section: Discussionmentioning

confidence: 99%

“…DNA fragmentation in carp erythrocytes was assessed by measuring the number of TUNEL-positive cells using the TdT-mediated dUTP nick end labeling (TUNEL) Apoptosis Assay Kit (Beyotime, Nantong, Jiangsu, China) as described previously by Monteiro et al (2009), with a minor modification. In brief, the cells were washed three times in PCS and fixed overnight in 4 % paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.…”

Section: Measurement Of Tunelmentioning

confidence: 99%

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Ca2+ and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian)

Feng

Jiang

et al. 2015

Fish Physiol Biochem

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Section: Discussionmentioning

confidence: 99%

Section: Measurement Of Tunelmentioning

confidence: 99%

Ca2+ and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian)

Feng

Jiang

et al. 2015

Fish Physiol Biochem

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“…The number of TUNEL-positive cells is a widely used index of DNA fragmentation [72,73]. DNA fragmentation may be closely associated with caspases in fish erythrocytes.…”

Section: • Oh-induced Apoptosis In Fish Erythrocytesmentioning

confidence: 99%

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Jiang

Liu

et al. 2016

Free Radical Biology and Medicine

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a b s t r a c tThe present study explored the apoptosis pathways in hydroxyl radicals ( • OH)-induced carp erythrocytes. Carp erythrocytes were treated with the caspase inhibitors in physiological carp saline (PCS) or Ca 2 þ -free PCS in the presence of 40 μM FeSO 4 /20 μM H 2 O 2 . The results showed that the generation of reactive oxygen species (ROS), the release of cytochrome c and DNA fragmentation were caspase-dependent, and Ca 2 þ was involved in calpain activation and phosphatidylserine (PS) exposure in • OH-induced carp erythrocytes. Moreover, the results suggested that caspases were involved in PS exposure, and Ca 2 þ was involved in DNA fragmentation in • OH-induced fish erythrocytes. These results demonstrated that there might be two apoptosis pathways in fish erythrocytes, one is the caspase and cytochrome c-dependent apoptosis that is similar to that in mammal nucleated cells, the other is the Ca 2 þ -involved apoptosis that was similar to that in mammal non-nucleated erythrocytes. So, fish erythrocytes may be used as a model for studying oxidative stress and apoptosis in mammal cells. Furthermore, the present study investigated the effects of glutamine (Gln)'s metabolites [alanine (Ala), citrulline (Cit), proline (Pro) and their combination (Ala 10 Pro 4 Cit 1 )] on the pathways of apoptosis in fish erythrocytes. The results displayed that Ala, Cit, Pro and Ala 10 Pro 4 Cit 1 effectively suppressed ROS generation, cytochrome c release, activation of caspase-3, caspase-8 and caspase-9 at the physiological concentrations, prevented Ca 2 þ influx, calpain activation, PS exposure, DNA fragmentation and the degradation of the cytoskeleton and oxidation of membrane and hemoglobin (Hb) and increased activity of anti-hydroxyl radical (AHR) in • OH-induced carp erythrocytes. Ala 10 Pro 4 Cit 1 produced a synergistic effect of inhibited oxidative stress and apoptosis in fish erythrocytes. These results demonstrated that Ala, Cit, Pro and their combination can protect mammal erythrocytes and nucleated cells against oxidative stress and apoptosis. The studies supported the use of Gln, Ala, Cit and Pro as oxidative stress and apoptosis inhibitors in mammal cells and the hypothesis that the inhibited effects of Gln on oxidative stress and apoptosis are at least partly dependent on that of its metabolites in mammalian.

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“…Based on our understanding, the disruption of gill epithelial cells is highly correlated with oxidative damage [19] and apoptosis [20] in fish. According to reports, the transcript abundance related to antioxidants and apoptosis may be regulated by NF-E2-related factor 2 (Nrf2) [21] and c-Jun N-terminal protein kinase (JNK) [22] in fish, respectively.…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary protein levels on the disease resistance, immune function and physical barrier function in the gill of grass carp ( Ctenopharyngodon idella ) after challenged with Flavobacterium columnare

Feng

Jiang

et al. 2016

Fish & Shellfish Immunology

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Copper toxicity in gills of the teleost fish, Oreochromis niloticus: Effects in apoptosis induction and cell proliferation

Cited by 81 publications

References 40 publications

Ca2+ and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian)

Ca2+ and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian)

The metabolites of glutamine prevent hydroxyl radical-induced apoptosis through inhibiting mitochondria and calcium ion involved pathways in fish erythrocytes

Effects of dietary protein levels on the disease resistance, immune function and physical barrier function in the gill of grass carp ( Ctenopharyngodon idella ) after challenged with Flavobacterium columnare

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