Copper toxicity and copper–zinc interactions in amphibian embryos

Herkovits, Jorge; Helguero, Luísa A.

doi:10.1016/s0048-9697(98)00184-3

Cited by 61 publications

(51 citation statements)

References 36 publications

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“…As a whole, the E 2 toxicity for Bufo arenarum embryos increases 2.23 times from 24 to 144 hr of exposure. This result is different from the toxicity pattern of some metals such as lead , copper (Herkovits and Helguero 1998), aluminum , and Cd on Bufo arenarum embryos, in which similar LC values were reported from 24 hr onward even up to 7 days (short-term chronic) of exposure. Other concentration thresholds such as LC 90 and LC 10 seem also to be meaningful for hazard assessment of E 2 because their 95% CIs in most cases do not overlap those of LC 50 (Table 1).…”

Section: Discussioncontrasting

confidence: 99%

Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos.

Fridman

Corró

Herkovits

2004

Environ Health Perspect

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The exposure of Bufo arenarum embryos to 25 µmol/L 17β-estradiol (E 2 ) resulted in 100% lethality within 48 hr, whereas 10 µmol//L E 2 was the no observed effect concentration value for shortterm chronic (7 days) exposure. The toxicity profile curves show that lethal effects were proportional to the E 2 concentration and the time of exposure. The E 2 uptake resulted in 20.1 ng E 2 /mg embryo at 8 hr posttreatment, but 67.3% of this value was achieved during the first 30 min of incubation with this estrogen. Regarding metabolism, the embryos synthesize estrone (E 1 ) from E 2 by means of 17β-hydroxysteroid dehydrogenase. Simultaneous treatments of Bufo arenarum embryos with 1 mg/L Cd 2+ and 0.1, 1, or 10 µmol/L E 2 enhanced the lethality exerted by cadmium in 76.7, 80, and 83.3% of embryos, respectively. The results indicate that estrogenic endocrine disruptors could have an adverse effect on amphibian embryos and enhance the toxic effect of Cd on amphibian embryos. This study points to the possibility of using the AMPHITOX test as a screening method for potential endocrine disruption as well as the combined effects of chemical mixtures.

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Section: Discussioncontrasting

confidence: 99%

Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos.

Fridman

Corró

Herkovits

2004

Environ Health Perspect

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“…In pregnant rats, copper exposure caused retardation of embryonic growth and differentiation, particularly affecting the neural tube (29). Copper is also a potent teratogen for amphibians (31,45). There are several reports describing the effects of copper on invertebrate development.…”

Section: Molecular Mechanisms Of Copper Toxicitymentioning

confidence: 99%

Physiological and toxicological transcriptome changes in HepG2 cells exposed to copper

Song

Freedman

2009

Physiological Genomics

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Copper is an essential trace element; however, at supraphysiological levels, it can be extremely toxic. Microarray data from HepG2 cells exposed to 100, 200, 400, and 600 μM copper for 4, 8, 12 and 24 h were generated and analyzed. Principal components, K-means, and hierarchical clustering, interactome, and pathway mapping analyses indicated that these exposure conditions induce physiological and toxicological changes in the HepG2 transcriptome. As a general trend, when the level of toxicity increases, the number and diversity of affected genes, Gene Ontology categories, regulatory pathways, and complexity of interactomes increase. Physiological responses to copper include transition metal ion binding and responses to stress/stimulus, whereas toxicological responses include apoptosis, morphogenesis, and negative regulation of biomolecule metabolism. The global gene expression profile was overlaid onto biomolecular interaction networks and signal transduction cascades using pathway mapping and interactome identification. This analysis indicated that copper modulates signal transduction pathways associated with MAPK, NF-κB, death receptor, IGF-I, hypoxia, IL-10, IL-2, IL-6, EGF, Toll-like receptor, protein ubiquitination, xenobiotic metabolism, leukocyte extravasation, complement and coagulation, and sonic hedgehog signaling. These results provide insights into the global and molecular mechanisms regulating the physiological and toxicological responses to metal exposure.

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“…Concentrations as low as 1-2 µg Cu /L produce adverse effects on aquatic biota [2]. In this context, the Cu concentrations employed as herbicides could represent a risk for "no target" organisms [2,3]. The main adverse effects of Cu at biochemical level occur on the structure and function of DNA and proteins, both in a direct way or mediated by means of oxidative stress mechanisms [4].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effects of Copper and Butylic Ester of 2,4-Dichlorophenoxyacetic Acid (Esternon Ultra) on Amphibian Embryos

Pérez-Coll

Herkovits

2006

IJERPH

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Abstract:Cu 2+ and butylic ester of 2,4-Dichlorophenoxyacetic acid as Esternon Ultra (2,4-D) toxicity on Bufo arenarum embryos were evaluated by means of a short-term chronic toxicity test (AMPHITOX). The NOEC values for Cu and 2,4-D were 0.02 mg/L and 2 mg/L respectively. The toxicity profile curves for Cu and 2,4-D were reported. The interactions of the metal and the herbicide were evaluated by combined treatments with different concentrations of Cu and 2,4-D. Although in all cases, a synergistic effect between these chemicals was observed, the combination of concentrations exerting low level effects in isolated treatments resulted in more adverse embryonic survival. Considering that both products are extensively used in agroecosystems, this fact could be of concern for non target species like amphibians.

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Copper toxicity and copper–zinc interactions in amphibian embryos

Cited by 61 publications

References 36 publications

Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos.

Estradiol uptake, toxicity, metabolism, and adverse effects on cadmium-treated amphibian embryos.

Physiological and toxicological transcriptome changes in HepG2 cells exposed to copper

Synergistic Effects of Copper and Butylic Ester of 2,4-Dichlorophenoxyacetic Acid (Esternon Ultra) on Amphibian Embryos

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