Copper toxicity and carbohydrate production in the microalgae<i>Cylindrotheca fusiformis</i>and<i>Gymnodinium</i>sp.

Pistocchi, Rossella; Guerrini, Franca; Balboni, V.; Boni, Laurita

doi:10.1080/09670269710001737049

Cited by 56 publications

(8 citation statements)

References 12 publications

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“…Ten milliliter culture was centrifuged at 12,000 × g for 10 min, and then 2 ml of supernatant was analyzed by the anthrone reagent method (Pistocchi et al, 1997). Glycerol content was determined according to the method of Chen et al (2011).…”

Section: Methodsmentioning

confidence: 99%

Salinity-Induced Palmella Formation Mechanism in Halotolerant Algae Dunaliella salina Revealed by Quantitative Proteomics and Phosphoproteomics

et al. 2017

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Palmella stage is critical for some unicellular algae to survive in extreme environments. The halotolerant algae Dunaliella salina is a good single-cell model for studying plant adaptation to high salinity. To investigate the molecular adaptation mechanism in salinity shock-induced palmella formation, we performed a comprehensive physiological, proteomics and phosphoproteomics study upon palmella formation of D. salina using dimethyl labeling and Ti4+-immobilized metal ion affinity chromatography (IMAC) proteomic approaches. We found that 151 salinity-responsive proteins and 35 salinity-responsive phosphoproteins were involved in multiple signaling and metabolic pathways upon palmella formation. Taken together with photosynthetic parameters and enzyme activity analyses, the patterns of protein accumulation and phosphorylation level exhibited the mechanisms upon palmella formation, including dynamics of cytoskeleton and cell membrane curvature, accumulation and transport of exopolysaccharides, photosynthesis and energy supplying (i.e., photosystem II stability and activity, cyclic electron transport, and C4 pathway), nuclear/chloroplastic gene expression regulation and protein processing, reactive oxygen species homeostasis, and salt signaling transduction. The salinity-responsive protein–protein interaction (PPI) networks implied that signaling and protein synthesis and fate are crucial for modulation of these processes. Importantly, the 3D structure of phosphoprotein clearly indicated that the phosphorylation sites of eight proteins were localized in the region of function domain.

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Section: Methodsmentioning

confidence: 99%

Salinity-Induced Palmella Formation Mechanism in Halotolerant Algae Dunaliella salina Revealed by Quantitative Proteomics and Phosphoproteomics

et al. 2017

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“…Oppositely, this study observed a reduction of the overall stressful effects of copper in intermediate concentrations of the co-cultivation in comparison to the single-species conditions. This may be related to the production of several extracellular agents with chelating functions, such as polysaccharides (Pistocchi et al, 1997) or metal-binding proteins (Sabatini et al, 2009), what may diminish the bioavailability of copper in the culture medium. At the highest copper concentration tested all growth parameters were affected, in co-cultivation and unialgal conditions, representing the toxic effects of copper in microalgae's metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, an attenuation effect on the metal's toxicity cannot be forwent. Despite this cell density difference, other factors may influence metal speciation and bioavailability in culture media, such as physicochemical aspects (pH and temperature), chemical and polarity affinity to other molecules and the presence of exudates with chelating properties, which have been described to be released by microalgae (Pistocchi et al, 1997;Sabatini et al, 2009). The effects of copper in the physiology of microalgae are well-known in the literature for a great variety of species.…”

Section: Discussionmentioning

confidence: 99%

Growth dynamic on a co-cultivation of two Chlorophyta microalgae exposed to copper

Dextro

2021

Acta Limnol. Bras.

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Aim Copper is an essential nutrient for the phytoplankton, but it can also act as a toxic agent, depending on its concentration. Considering the continuous increase of this metal in the natural aquatic ecosystems, understanding its actions in co-cultivation scenarios is of great relevance. Experiments with the combination of different species resemble more accurately the natural conditions, in contrast of results obtained in single-species tests, which cannot be directly used to describe observed effects on the environment. Methods Therefore, growth parameters were investigated and compared on the co-cultivation of Chlorella sorokiniana and Kirchneriella obesa and their separate cultures exposed to three different free copper concentrations (control 6x10-9, intermediate 2x10-7 and high 1.5x10-6 mol.L-1 Cu2+). Results C. sorokiniana registered more cells in the control of the unialgal culture while K. obesa had higher cell density in the control of the co-cultivation. Growth rates decreased with the increment of copper in the unialgal conditions. However, both species maintained a high growth rate in the co-cultivation intermediate copper concentrations. Biovolume varied despite the cultivation method, being strongly related to the metal’s concentration. The maximum photosynthetic efficiency decreased in higher copper. Conclusions According to the results observed, no competitive exclusion occurred and both species were affected by copper in unialgal and co-cultivation conditions, with K. obesa being favored by the co-cultivation, which seems to have an attenuation effect on copper toxicity until intermediate concentrations. Ecologically, the results suggest that communities deal better with the toxic effects caused by intermediate copper concentrations than single-species cultures.

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“…Several Peridinium, Prorocentrum, and Thoracosphaera species displayed completely suppressed growth around pCu 10.5. Prior to this, Schenck (1984) documented complete inhibition of Gonyaulax tamarensis at pCu 11, and Pistocchi et al (1997) later confirmed this sensitivity, noting complete inhibition of Prorocentrum micans and Gonyaulax polyedra at pCu 10.7. Anderson and Morel (1978), however, found that pCu 9.7 was necessary to reduce motility of a coastal Gonyaulax tamarensis isolate.…”

Section: Dinoflagellates (Figure 4c)mentioning

confidence: 91%

The Toxicity of Copper to Crocosphaera watsonii and Other Marine Phytoplankton: A Systematic Review

2019

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The cupric ion (Cu 2+) plays a dual role as both nutrient and toxicant to freshwater and marine phytoplankton, functioning in multiple photochemical processes, as well as reactive oxygen species (ROS) production. This duality has been investigated through a variety of methods to determine the consequences of natural and anthropogenic copper introduction to algal ecosystem composition. Studies conducted over the past few decades have described the growth responses of many unique organisms to copper availability. Such observations are critical for describing the global distributions of major phytoplankton species in terms of global trace metal abundance; however, highly variable experimental practices impede direct inter-study comparison. The aim of this systematic review is to summarize the available data regarding the effects of copper concentration on diverse marine phytoplankton growth rates. Through extensive literary comparison, 143 studies were systematically reviewed, and data on copper concentrations and growth rates reported were extracted. From the data available, we conclude that trends in phytoplankton sensitivity to copper are mainly driven by a single study. We discuss the obstacles to inter-study comparison and detail both the concurring and conflicting results to date, with an emphasis on taxonomic trends and methodologies employed. Finally, we present the first copper sensitivity measurements for marine unicellular diazotrophs using three representative strains of the unicellular nitrogen-fixing cyanobacterium, Crocosphaera watsonii.

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Copper toxicity and carbohydrate production in the microalgaeCylindrotheca fusiformisandGymnodiniumsp.

Cited by 56 publications

References 12 publications

Salinity-Induced Palmella Formation Mechanism in Halotolerant Algae Dunaliella salina Revealed by Quantitative Proteomics and Phosphoproteomics

Salinity-Induced Palmella Formation Mechanism in Halotolerant Algae Dunaliella salina Revealed by Quantitative Proteomics and Phosphoproteomics

Growth dynamic on a co-cultivation of two Chlorophyta microalgae exposed to copper

The Toxicity of Copper to Crocosphaera watsonii and Other Marine Phytoplankton: A Systematic Review

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