Copper tolerant yeasts isolated from polluted area of Argentina

Villegas, Liliana Beatriz; Amoroso, Maria Julia del R.; Figueroa, Lucía I. C. de

doi:10.1002/jobm.200510569

Cited by 45 publications

(63 citation statements)

References 22 publications

(22 reference statements)

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“…Samples of 20-30 g were randomly selected, transported in sterile plastic bags, kept at 4°C until use and processed as described previously by Villegas et al (2005). Micromorphology and colonial features were examined and reproductive forms were tested in different media to determine whether the isolates belonged to the same group.…”

Section: Sampling and Isolationmentioning

confidence: 99%

Chromate removal by yeasts isolated from sediments of a tanning factory and a mine site in Argentina

et al. 2008

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Twenty-one yeast-like microorganisms were isolated from tannery effluents and from a nickel-copper mine in Argentina. They were tested for their Cu(II), Ni(II), Cd(II) and Cr(VI) tolerance in qualitative assays on solid medium. Three isolates were selected for their multiple tolerance to the different heavy metals and highest tolerance to Cr(VI). According to morphological and physiological analysis and 26S rDNA D1/D2 domain sequences the isolates were characterized as: Lecythophora sp. NGV-1, Candida sp. NGV-9 and Aureobasidium pullulans VR-8. Resistance of the three strains to high Cr(VI) concentrations and their ability to remove Cr(VI) were assessed using YNB-glucose medium supplemented with 0.5 and 1 mM Cr(VI). Chromate removal activity was estimated by measuring remaining Cr(VI) concentration in the supernatant using the colorimetric 1,5-diphenylcarbazide method and total chromium was determined by flame atomic absorption spectroscopy. The results indicate that the initial Cr(VI) concentration negatively influenced growth and the specific growth rate but stimulated the metabolic activity of the three strains; resistance to Cr(VI) by these strains was mainly due to reduction of Cr(VI) rather than chromium bioaccumulation. This study showed the potential ability of these strains as tools for bioremediation of Cr(VI) from contaminated sites.

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Section: Sampling and Isolationmentioning

confidence: 99%

Chromate removal by yeasts isolated from sediments of a tanning factory and a mine site in Argentina

et al. 2008

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“…The same has been observed for fungi at concentration of Zn ranging from 100-400 mg L 21 [35], and for copper removal by Rhodotorula mucilaginosa RCL-11 and Candida sp. RCL-3 [36].…”

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- Biodegradability of Oily Wastewater Using Rotating Biological Contactor Combined with an External Membrane

Monsalvo¹

2015

Ecological Technologies for Industrial Wastewater Management

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In this study was developed a natural process using a biological system for the biosynthesis of nanoparticles (NPs) and possible removal of copper from wastewater by dead biomass of the yeast Rhodotorula mucilaginosa. Dead and live biomass of Rhodotorula mucilaginosa was used to analyze the equilibrium and kinetics of copper biosorption by the yeast in function of the initial metal concentration, contact time, pH, temperature, agitation and inoculum volume. Dead biomass exhibited the highest biosorption capacity of copper, 26.2 mg g 21 , which was achieved within 60 min of contact, at pH 5.0, temperature of 30uC, and agitation speed of 150 rpm. The equilibrium data were best described by the Langmuir isotherm and Kinetic analysis indicated a pseudo-second-order model. The average size, morphology and location of NPs biosynthesized by the yeast were determined by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and transmission electron microscopy (TEM). The shape of the intracellularly synthesized NPs was mainly spherical, with an average size of 10.5 nm. The X-ray photoelectron spectroscopy (XPS) analysis of the copper NPs confirmed the formation of metallic copper. The dead biomass of Rhodotorula mucilaginosa may be considered an efficiently bioprocess, being fast and low-cost to production of copper nanoparticles and also a probably nano-adsorbent of this metal ion in wastewater in bioremediation process.

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“…This microorganism is capable of accumulating up to 44 % of the copper from a medium supplemented with 0.5 mM CuSO 4 as a Cu(II) source (Villegas et al 2005 ). After 48 h of growth in the presence of Cu(II), electron microscopy showed dark grains in the cytoplasm of R. mucilaginosa RCL-11.…”

Section: Rhodototula Mucilaginosa Rcl-11mentioning

confidence: 99%

“…Rhodotorula mucilaginosa RCL-11 was isolated from fi lter plant of a copper mine located in the NorthWest of Argentina (Villegas et al 2005 ). This microorganism is capable of accumulating up to 44 % of the copper from a medium supplemented with 0.5 mM CuSO 4 as a Cu(II) source (Villegas et al 2005 ).…”

Section: Rhodototula Mucilaginosa Rcl-11mentioning

confidence: 99%

Copper Resistance and Oxidative Stress Response in Rhodotorula mucilaginosa RCL-11. Yeast Isolated from Contaminated Environments in Tucumán, Argentina

Irazusta

Figueroa

2014

Bioremediation in Latin America

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The threat of heavy metal pollution to public health and wildlife has led to a great interest in the development of effective technologies for heavy metal immobilization in a non-bioavailable form or their conversion into less toxic forms. Organisms subjected to metal exposure in their natural environments have developed resistance mechanisms such us dedicated components and sophisticated homeostasis. Rhodotorula mucilaginosa RCL-11, a pigmented yeast isolated from a fi lter plant of a copper mine in the province of Tucumán, Argentina, supports high concentrations of the heavy metal Cu(II). In order to understand the mechanism involved in resistance to copper in this yeast, a proteomic study was conducted. Identifi cation of differentially expressed proteins was performed. The results obtained show that when R. mucilaginosa RCL-11 was exposed to 0.5 mM copper, differential proteins, involved in cell resistance mechanisms, were expressed. Moreover, copper overload augmented carotenoid biosynthesis in this yeast, modifying at the same time the relative proportion of the pigments produced. Inhibition of the synthesis pathway with diphenylamine suggests an inverse relationship between carotenoid and copper biosorption by R. mucilaginosa RCL-11. The increased activity of superoxide dismutase and catalase measured under inhibition of carotenoid biosynthesis could explain these observations. The change in the relative proportion of the carotenoids torularhodin, torulene, and beta-carotene, as well as the detection of gamma-carotene in the presence of Cu(II) allows to hypothesize that the carotenoids produced by R. mucilaginosa RCL-11 play different roles in the oxidative stress response of this yeast.

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Copper tolerant yeasts isolated from polluted area of Argentina

Cited by 45 publications

References 22 publications

Chromate removal by yeasts isolated from sediments of a tanning factory and a mine site in Argentina

Chromate removal by yeasts isolated from sediments of a tanning factory and a mine site in Argentina

- Biodegradability of Oily Wastewater Using Rotating Biological Contactor Combined with an External Membrane

Copper Resistance and Oxidative Stress Response in Rhodotorula mucilaginosa RCL-11. Yeast Isolated from Contaminated Environments in Tucumán, Argentina

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