Copper Sources for Sod1 Activation

Boyd, Stefanie D.; Ullrich, Morgan S.; Skopp, Amélie; Winkler, Duane D.

doi:10.3390/antiox9060500

Cited by 27 publications

(18 citation statements)

References 110 publications

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“…On the other hand, we found that 25 µM D-Pen had little effect on the downstream signaling pathways. The maintenance of cell homeostasis may be a key factor in determining the related proteins of the signaling pathway (Sun et al, 2010;Galano et al, 2015;Boyd et al, 2020). Although the MTT results did not show the effect of D-Pen on the cells survival, the activity of LDH increased and the activity of SOD decreased in the 25 µM D-Pen medium (data not shown).…”

Section: Discussionmentioning

confidence: 91%

Nasal Delivery of D-Penicillamine Hydrogel Upregulates a Disintegrin and Metalloprotease 10 Expression via Melatonin Receptor 1 in Alzheimer’s Disease Models

Zhong

Kou

Zhao

et al. 2021

Front. Aging Neurosci.

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Alzheimer’s disease (AD) is a type of neurodegenerative disease that is associated with the accumulation of amyloid plaques. Increasing non-amyloidogenic processing and/or manipulating amyloid precursor protein signaling could reduce AD amyloid pathology and cognitive impairment. D-penicillamine (D-Pen) is a water-soluble metal chelator and can reduce the aggregation of amyloid-β (Aβ) with metals in vitro. However, the potential mechanism of D-Pen for treating neurodegenerative disorders remains unexplored. In here, a novel type of chitosan-based hydrogel to carry D-Pen was designed and the D-Pen-CS/β-glycerophosphate hydrogel were characterized by scanning electron microscopy and HPLC. Behavior tests investigated the learning and memory levels of APP/PS1 mice treated through the D-Pen hydrogel nasal delivery. In vivo and in vitro findings showed that nasal delivery of D-Pen-CS/β-GP hydrogel had properly chelated metal ions that reduced Aβ deposition. Furthermore, D-Pen mainly regulated A disintegrin and metalloprotease 10 (ADAM10) expression via melatonin receptor 1 (MTNR1α) and the downstream PKA/ERK/CREB pathway. The present data demonstrated D-Pen significantly improved the cognitive ability of APP/PS1 mice and reduced Aβ generation through activating ADAM10 and accelerating non-amyloidogenic processing. Hence, these findings indicate the potential of D-Pen as a promising agent for treating AD.

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Section: Discussionmentioning

confidence: 91%

Nasal Delivery of D-Penicillamine Hydrogel Upregulates a Disintegrin and Metalloprotease 10 Expression via Melatonin Receptor 1 in Alzheimer’s Disease Models

Zhong

Kou

Zhao

et al. 2021

Front. Aging Neurosci.

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“…Alternatively, since copper is a known cofactor for the copper/zinc superoxide dismutase 1 (SOD1), an enzyme involved in protecting the cells from reactive species produced during cellular respiration, 28–30 copper might have increased the cellular antioxidant defense capacity in a similar manner as it was suggested for manganese, 12 leading to an increased cell growth and fusion protein production. SOD1 is preventing cellular lipid peroxidation and deoxyribonucleic acid (DNA) damages by dismutating superoxide anions and is thus an essential enzyme of the cellular antioxidant defense system 30 …”

Section: Discussionmentioning

confidence: 99%

Copper impurity of iron raw material contributes to improved cell culture performance

Weiss

Caspari

Merkel

et al. 2022

Biotechnology Progress

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Cell culture medium (CCM) formulations are chemically defined to reduce lot‐to‐lot variability and complexity of the medium while still providing all essential nutrients supporting cell growth and productivity of various cell lines. However, raw material impurities may still introduce variations and inconsistencies to final CCM formulations. In one of our previous studies (Weiss et al. Biotechnol Prog. 2021;37(4):e3148), we have demonstrated the impact of iron raw material impurity on Chinese hamster ovary (CHO) cell performance and critical quality attributes (CQAs) of recombinant proteins within the Cellvento® 4CHO CCM platform by identifying manganese impurity as the main root cause for improved cell performance and altered glycosylation profiles. This study sought to investigate the impact of iron raw material impurities within another medium platform, namely EX‐CELL® Advanced CHO Fed‐Batch‐Medium. As opposed to previously published results, in this platform, copper instead of manganese impurity present within the used ferric ammonium citrate (FAC) iron source was responsible for an improved cell performance of a CHOZN® cell line and a slight difference in CQAs of the produced recombinant protein. The use of tightly controlled raw material specifications or the use of low impurity iron sources is therefore crucial to minimize the impact of impurities on cell performance in any CCM platform and thereby guarantee consistent and reproducible cell culture processes.

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“…It has the largest kcat/KM of any known enzyme (≈7 × 10 9 M −1 s −1 ) and retains its activity in a wide range of pH and temperature values [ 87 ]. The level of active holo-SOD1 is backed up by the presence of a permanent apo-SOD1 pool, which can be converted to holo-SOD by transferring a copper ion from the Cu(I)-chaperone to SOD1 (CCS) that obtains copper directly from CTR1 at the cell membrane [ 88 , 89 ]. Thus, damping the activity of SOD1 is not an easy task.…”

Section: The Points In Which Iav-induced Inflammation Cross With Copper Metabolismmentioning

confidence: 99%

The Crossroads between Host Copper Metabolism and Influenza Infection

Puchkova

Kiseleva

Polishchuk

et al. 2021

IJMS

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Three main approaches are used to combat severe viral respiratory infections. The first is preemptive vaccination that blocks infection. Weakened or dead viral particles, as well as genetic constructs carrying viral proteins or information about them, are used as an antigen. However, the viral genome is very evolutionary labile and changes continuously. Second, chemical agents are used during infection and inhibit the function of a number of viral proteins. However, these drugs lose their effectiveness because the virus can rapidly acquire resistance to them. The third is the search for points in the host metabolism the effect on which would suppress the replication of the virus but would not have a significant effect on the metabolism of the host. Here, we consider the possibility of using the copper metabolic system as a target to reduce the severity of influenza infection. This is facilitated by the fact that, in mammals, copper status can be rapidly reduced by silver nanoparticles and restored after their cancellation.

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Copper Sources for Sod1 Activation

Cited by 27 publications

References 110 publications

Nasal Delivery of D-Penicillamine Hydrogel Upregulates a Disintegrin and Metalloprotease 10 Expression via Melatonin Receptor 1 in Alzheimer’s Disease Models

Nasal Delivery of D-Penicillamine Hydrogel Upregulates a Disintegrin and Metalloprotease 10 Expression via Melatonin Receptor 1 in Alzheimer’s Disease Models

Copper impurity of iron raw material contributes to improved cell culture performance

The Crossroads between Host Copper Metabolism and Influenza Infection

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