Copper(<scp>ii</scp>)-doped semiconducting polymer dots for nitroxyl imaging in live cells

Wu, Xu; Li, Wu; Wu, I‐Chin; Chiu, Daniel T.

doi:10.1039/c6ra20439b

Cited by 10 publications

(3 citation statements)

References 40 publications

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“…The preparation of the barcoding Ln-Pdots was similar as described in our previous reports with minor modification. , Briefly, the carboxyl-functionalized PFBT (synthesized as indicated in our previous report) was used to coordinate with a mixture of lanthanide ions, including Tb, Ho, and Tm (Sigma-Aldrich, St. Louis, MO) at different ratios in THF for 1 h. With the addition of PS–PEG–COOH (Polymer Source Inc., Quebec, Canada), the THF solution was quickly injected into water with ultrasonication to form the Ln-Pdots. After removing THF by nitrogen stripping, the Ln-Pdots solution was filtered through a 0.45 μm cellulose membrane filter (VWR, Radnor, PA).…”

Section: Experiments and Materialsmentioning

confidence: 99%

Ratiometric Barcoding for Mass Cytometry

DeGottardi

et al. 2018

Anal. Chem.

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Barcoding is of importance for high-throughput cellular and molecular analysis. A ratiometric barcoding strategy using lanthanide-coordinated polymer dots (Ln-Pdots) was developed for mass cytometric analysis. By using 3 metal isotopes and 4 ratio intensity levels, 16 barcodes were generated to code, and later decode, cell samples in mass cytometry. The ratiometric Ln-Pdot barcodes not only provided high-mass-signal intensities but also eliminated the bias caused by different concentrations of the labeling reagents/barcodes and run-to-run differences in cell labeling efficiency. The ability to distinguish clearly the 16 sets of labeled MCF-7 cells with mass cytometry demonstrated the excellent resolving power of the ratiometric Ln-Pdot barcodes. Furthermore, the results from barcoding PBMC samples via CD45-specific cellular targeting indicated that the ratiometric Ln-Pdot barcodes could facilitate mass cytometry in high-throughput and multiplexed analysis, especially with precious human samples.

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Section: Experiments and Materialsmentioning

confidence: 99%

Ratiometric Barcoding for Mass Cytometry

DeGottardi

et al. 2018

Anal. Chem.

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“…[8] Meanwhile, the metal ions in the nanoparticle could easily access the small molecules, such as HNO, to execute the catalytic reaction. [9] Therefore, we believe that the doping of Fe 2+ in those polymer nanoparticles can solve the challenges in CDT. [10] It is well-known that tumor cells need more nutrients to maintain their uncontrolled growth than normal cells.…”

Section: Introductionmentioning

confidence: 99%

Iron‐Rich Semiconducting Polymer Dots for the Combination of Ferroptosis–Starvation and Phototherapeutic Cancer Therapy

Chen

Yang

Tang

et al. 2023

Adv Healthcare Materials

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Chemodynamic therapy (CDT) has emerged as an outstanding antitumor therapeutic method due to its selectivity and utilization of tumor microenvironment. However, there are still unmet requirements to achieve a high antitumor efficiency, including the tumor accumulation of catalyst and enrichment of reactants of Fenton reaction. Here, an iron‐loaded semiconducting polymer dot modified with glucose oxidase (Pdot@Fe@GOx) is reported to deliver iron ions into tumor tissues and in situ generation of hydrogen peroxide in tumors. On one hand, Pdot@Fe@GOx converts glucose to gluconic acid and hydrogen peroxide (H2O2) in tumor, which not only consumes glucose of tumor cells, but also provides the H2O2 for the following Fenton reaction. On the other hand, the Pdot@Fe@GOx delivers active iron ions in tumor to perform CDT with the combination of the generated H2O2. In addition, the Pdot@Fe@GOx has both photothermal and photodynamic effects under the irradiation of near‐infrared laser, which can improve and compensate the CDT effect to kill cancer cells. This Pdot@Fe@GOx‐based multiple‐mode therapeutic strategy has successfully achieved a synergistic anticancer effect with minimal side effects and has the potential to be translated into preclinical setting for tumor therapy.

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“…Semiconducting polymer dots (Pdots) derived from a fluorescent conjugated polymer − display unique characteristics that are very favorable for fluorescence sensing and imaging, including high single-particle brightness, excellent photostability, and low cytotoxicity, − and have been exploited in sensing, − imaging, ,− and photodynamic therapy. , However, Pdots modified via tuning the surface chemistry as a subcellular targeting probe for mitochondrial pH monitoring has not been reported so far to the best our knowledge. In this work, we designed the first example of pH-responsive and mitochondria-targeted functionalized Pdots for pH mit monitoring and imaging in living cells (Scheme ).…”

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confidence: 99%

A Mitochondria-Targeted Ratiometric Biosensor for pH Monitoring and Imaging in Living Cells with Congo-Red-Functionalized Dual-Emission Semiconducting Polymer Dots

2017

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The accurate and sensitive monitoring and imaging of mitochondrial pH in living cells play vital roles in chemical biology and biomedicine. Herein, we design a novel ratiometric fluorescent chemical probe for monitoring and imaging the pH of mitochondria in living cells based on congo-red (CR)-modified dual-emission semiconducting polymer dots (Pdots) via a competitive fluorescence resonance energy transfer (FRET) mechanism. The Pdots are synthesized by triphenylphosphonium (TPP)-modified polyoxyethylene nonylphenylether (CO-520), poly(9,9-dioctylfluorenyl-2,7-diyl) (PFO), poly(9,9-dioctylfluorene)-co-(4,7-di-2-thienyl-2,1,3-benzothiadiazole) (PF-DBT5), and poly(styrene-co-maleic anhydride) (PSMA) via a nanoprecipitation method, and the prepared Pdots are further chemically linked with pH-sensitive, nonfluorescent CR to obtain the mitochondria-targeted pH fluorescent probes. This Pdots-based probe consists of two luminescent components including PFO and PF-DBT5 as fluorescence donors, an acid-base indicator CR as an energy acceptor, and TPP as the mitochondria-targeting group. At a different pH region, the FRET efficiency between CR and PFO or CR and PF-DBT5 can be modulated. This probe exhibits good biocompatibility, a wide pH detection range from 2.57 to 8.96, good reversibility, high selectivity, and excellent photostability for pH monitoring. This probe allows for the detecting and imaging of mitochondrial pH in living cells with satisfactory results.

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Copper(ii)-doped semiconducting polymer dots for nitroxyl imaging in live cells

Cited by 10 publications

References 40 publications

Ratiometric Barcoding for Mass Cytometry

Ratiometric Barcoding for Mass Cytometry

Iron‐Rich Semiconducting Polymer Dots for the Combination of Ferroptosis–Starvation and Phototherapeutic Cancer Therapy

A Mitochondria-Targeted Ratiometric Biosensor for pH Monitoring and Imaging in Living Cells with Congo-Red-Functionalized Dual-Emission Semiconducting Polymer Dots

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