Copper resistance determinants in bacteria

Brown, Nigel L.; Rouch, Duncan A.; Lee, Barry T.O.

doi:10.1016/0147-619x(92)90005-u

Cited by 115 publications

(92 citation statements)

References 27 publications

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“…Among various transition metals, such two-component systems have been identified for copper and silver. Two different copper-responsive two-component systems are present in E. coli, namely, CusS/CusR (34), and PcoS/PcoR (35). In Pseudomonas syringae, a plant pathogen, the CopS/CopR two-component system provides copper resistance (36), whereas in Salmonella, the SilS/SilR system provides resistance to silver ions (37).…”

Section: Discussionmentioning

confidence: 99%

A Two-component Signal Transduction Pathway Regulates Manganese Homeostasis in Synechocystis 6803, a Photosynthetic Organism

Ogawa

Bao

Katoh

et al. 2002

Journal of Biological Chemistry

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Elemental manganese is essential for the production of molecular oxygen by cyanobacteria, plants, and algae. In the cyanobacterium Synechocystis sp. PCC 6803, transcription of the mntCAB operon, encoding a high affinity Mn transporter, occurs under Mn starvation (nM Mn) conditions but not in Mn-sufficient (M Mn) growth medium. Using a strain in which the promoter of this operon directs the transcription of the luxAB reporter genes, we determined that inactivation of the slr0640 gene, which encodes a histidine kinase sensor protein component of a two-component signal transduction system, resulted in constitutive high levels of lux luminescence. Systematic targeted inactivation mutagenesis also identified slr1837 as the gene encoding the corresponding response regulator protein. We have named these two genes manS (manganese-sensor) and manR (manganese-regulator), respectively. A polyhistidinetagged form of the ManS protein was localized in the Synechocystis 6803 cell membrane. Directed replacement of the conserved catalytic His-205 residue of this protein by Leu abolished its activity, although the mutated protein was present in cyanobacterial membrane. This mutant also showed suboptimal rates of Mn uptake under either Mn-starved or Mn-sufficient growth condition. These data suggest that the ManS/ManR two-component system plays a central role in the homeostasis of manganese in Synechocystis 6803 cells.

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Section: Discussionmentioning

confidence: 99%

A Two-component Signal Transduction Pathway Regulates Manganese Homeostasis in Synechocystis 6803, a Photosynthetic Organism

Ogawa

Bao

Katoh

et al. 2002

Journal of Biological Chemistry

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“…tomato, the copABCD operon encodes the structural resistance genes, and is transcribed from a copper-inducible promoter controlled by the two-component regulatory module copRS (Mills et al, 1994). In E. coli, the plasmid-borne pcoABCD pcoSR pcoE locus is involved in periplasmic copper handling (Brown et al, 1992;Lee et al, 2002). The additional gene pcoE encodes a peptide involved in the periplasmic binding of copper ions, and is regulated by a separate copper-regulated promoter (Rensing & Grass, 2003).…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic and proteomic analyses of the pMOL30-encoded copper resistance in Cupriavidus metallidurans strain CH34

et al. 2006

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The four replicons of Cupriavidus metallidurans CH34 (the genome sequence was provided by the US Department of Energy–University of California Joint Genome Institute) contain two gene clusters putatively encoding periplasmic resistance to copper, with an arrangement of genes resembling that of the copSRABCD locus on the 2.1 Mb megaplasmid (MPL) of Ralstonia solanacearum, a closely related plant pathogen. One of the copSRABCD clusters was located on the 2.6 Mb MPL, while the second was found on the pMOL30 (234 kb) plasmid as part of a larger group of genes involved in copper resistance, spanning 17 857 bp in total. In this region, 19 ORFs (copVTMKNSRABCDIJGFLQHE) were identified based on the sequencing of a fragment cloned in an IncW vector, on the preliminary annotation by the Joint Genome Institute, and by using transcriptomic and proteomic data. When introduced into plasmid-cured derivatives of C. metallidurans CH34, the cop locus was able to restore the wild-type MIC, albeit with a biphasic survival curve, with respect to applied Cu(II) concentration. Quantitative-PCR data showed that the 19 ORFs were induced from 2- to 1159-fold when cells were challenged with elevated Cu(II) concentrations. Microarray data showed that the genes that were most induced after a Cu(II) challenge of 0.1 mM belonged to the pMOL30 cop cluster. Megaplasmidic cop genes were also induced, but at a much lower level, with the exception of the highly expressed MPL copD. Proteomic data allowed direct observation on two-dimensional gel electrophoresis, and via mass spectrometry, of pMOL30 CopK, CopR, CopS, CopA, CopB and CopC proteins. Individual cop gene expression depended on both the Cu(II) concentration and the exposure time, suggesting a sequential scheme in the resistance process, involving genes such as copK and copT in an initial phase, while other genes, such as copH, seem to be involved in a late response phase. A concentration of 0.4 mM Cu(II) was the highest to induce maximal expression of most cop genes.

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“…The molecular responses to copper toxicity have been investigated for many organisms, including bacteria, plants, yeasts, and mammals (e.g. Grill et al, 1989;Zhou and Goldsbrough, 1994; reviewed by Rauser, 1990;Steffens, 1990;Brown et al, 1992;Zhou and Thiele, 1993;Solioz et al, 1994). Accordingly, different mechanisms of adaptation to toxicity have been described and are being characterized in ongoing research efforts.…”

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confidence: 99%

Regulated Copper Uptake in Chlamydomonas reinhardtii in Response to Copper Availability

et al. 1996

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A saturable and temperature-dependent copper uptake pathway has been identified in Chlamydomonas reinhardtii. The uptake system has a high affinity for copper ions (Km approximately 0.2 [mu]M) and is more active in cells that are adapted to copper deficiency than to cells grown in a medium containing physiological (submicromolar to micromolar) copper ion concentrations. The maximum velocity of copper uptake by copper-deficient cells (169 pmol h-1 106 cells-1 or 62 ng min-1 mg-1 chlorophyll) is up to 20-fold greater than that of fully copper-supplemented cells, and the Km (approximately 2 x 102 nM) is unaffected. Thus, the same uptake system appears to operate in both copper-replete and copper-deficient cells, but its expression or activity must be induced under copper-deficient conditions. A cupric reductase activity is also increased in copper-deficient compared with copper-sufficient cells. The physiological characteristics of the regulation of this cupric reductase are compatible with its involvement in the uptake path-way. Despite the operation of the uptake pathway under both copper-replete and copper-deficient conditions, C. reinhardtii cells maintained in fully copper-supplemented cells do not accumulate copper in excess of their metabolic need. These results provide evidence for a homeostatic mechanism for copper metabolism in C. reinhardtii.

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Copper resistance determinants in bacteria

Cited by 115 publications

References 27 publications

A Two-component Signal Transduction Pathway Regulates Manganese Homeostasis in Synechocystis 6803, a Photosynthetic Organism

A Two-component Signal Transduction Pathway Regulates Manganese Homeostasis in Synechocystis 6803, a Photosynthetic Organism

Transcriptomic and proteomic analyses of the pMOL30-encoded copper resistance in Cupriavidus metallidurans strain CH34

Regulated Copper Uptake in Chlamydomonas reinhardtii in Response to Copper Availability

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