Copper Resistance and Accumulation in the Zygomycete Mucor rouxii

Ramirez-Salgado, P; Caracheo-Aguilar, S; Obregón-Herrera, Armando; Sosa-Luna, L; Amezquita-Lopez, F; Mendoza-Dı́az, Guillermo; Gutiérrez-Corona, Félix

doi:10.1007/s002849900094

Cited by 13 publications

(8 citation statements)

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“…These isolates were grown on seven copper sulphate concentrations (0, 0·3, 0·6, 1·2, 2·4, 3·2 and 4·8 m m ). In a previous study with the dimorphic fungus Mucor rouxii , copper sulphate concentrations of 3·2, 4·8 and 6·0 m m were considered to be high, and strains whose spores could germinate and grow on 6·0 m m were considered resistant (Ramirez‐Salgado et al. , 1996).…”

Section: Resultsmentioning

confidence: 99%

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Discriminating microsatellites from Macrophomina phaseolina and their potential association to biological functions

Arias

Ray²,

Mengistu³

et al. 2011

Plant Pathology

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One hundred and eighty-two microsatellites or simple-sequence-repeat (SSR) markers for Macrophomina phaseolina were developed. These were tested on 24 isolates of M. phaseolina obtained from seven plant species, and the genetic variation of isolates was studied in relation to potential biological processes that could be affected in this fungus. A total of 120 SSR markers were polymorphic, amplifying >90% of the 24 isolates tested. Thirty percent of the markers showed multiple alleles on individual samples. A large number of markers showed unique alleles in isolates collected from pumpkin and snap bean. DNA sequences corresponding to 43 markers had significant hits on BLASTx and⁄ or BLAST2GO, and the polymorphism of 36 of those markers showed specific allele patterns for one or more plant host origin of the isolates. Additional tests on growth rate and copper resistance of the isolates identified markers that could be related to those traits. In addition, 27 markers were monomorphic and amplified all 24 isolates. Whereas polymorphic markers can be used for population genetics studies of M. phaseolina, the group of 27 monomorphic markers could help in the fast identification of this species in clinical specimens. The SSR markers developed here will enrich the limited molecular marker resource in M. phaseolina and could be used as the basis for more in-depth studies of the host-pathogen interactions of M. phaseolina.

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Section: Resultsmentioning

confidence: 99%

“…1). Fungi have developed numerous mechanisms for copper resistance (Cervantes & Gutierrez‐Corona, 1994) some of them involving cell wall composition, and in some fungi copper resistance has been related to greater ability of spore germination (Ramirez‐Salgado et al. , 1996).…”

Section: Discussionmentioning

confidence: 99%

Discriminating microsatellites from Macrophomina phaseolina and their potential association to biological functions

Arias

Ray²,

Mengistu³

et al. 2011

Plant Pathology

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“…Elements like copper and zinc are essential micronutrients and may have specific roles in morphogenesis at low and therefore nontoxic concentrations, but some morphological changes may occur at concentrations where some toxicity may be manifest. Earlier studies indicated that living cells of a copper-tolerant strain of M. rouxii accumulated higher amounts of the metal as compared to the metal sensitive parental strain [17,18]. However, no studies on the binding of copper ions by inactivated cells obtained from copper-tolerant and copper-sensitive strains of M. rouxii have been reported when the fungal cells have been cultured under trace and high concentrations of Cu 2ϩ .…”

Section: Introductionmentioning

confidence: 99%

Enhanced Copper Adsorption and Morphological Alterations of Cells of Copper-Stressed Mucor Rouxii

Gardea‐Torresdey¹,

Cano-Aguilera²,

Webb³

et al. 1997

Environ Toxicol Chem

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Abstract-Fungi are ubiquitous and can become dominant in metal-polluted habitats. Some fungal strains are tolerant to metal toxicity. The presence of a high copper concentration in the culture medium induced morphological changes in the copper-tolerant strain of Mucor rouxii. Copper binding by strains of M. rouxii cultured at a trace (1.6 ϫ 10 Ϫ3 mM) copper concentration was less effective than those cultured at a high (3.2 mM) copper concentration. These experiments were performed at an optimum time of 30 min and a pH of 5 for Cu 2ϩ binding. Our results suggest that a passive metal-binding mechanism makes the majority of total copper binding. These preliminary findings suggest that the presence of high levels of copper in the culture medium allow the development of chemical functional groups on the fungal surface, which led to an enhanced copper-binding ability and induced important morphological changes in M. rouxii.

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“…The cell wall composition and the chitin content have an important role in tolerance mechanisms in fungi. At 3·2 mmol kg −1 of CuSO 4 , the copper‐resistant strains of Mucor rouxii bounded about 15% more copper in the cell walls than in the cytosol and membranes (Ramirez‐Salgado et al. 1996).…”

Section: Discussionmentioning

confidence: 99%

Modelling the inhibitory effect of copper sulfate on the growth of Penicillium expansum and Botrytis cinerea

Judet-Correia

Charpentier

Bensoussan

et al. 2011

Letters in Applied Microbiology

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Aims: This study aimed to investigate the effect of copper sulfate (from 0 to 8 mmol kg−1) on radial growth rate and lag time of two moulds responsible for vine grapes spoilage: Penicillium expansum strain 25·03 and Botrytis cinerea, strains BC1 and BC2. Methods and results: A new model was developed to describe tailing and shoulders in the inhibition curves. Because of tailing, the minimum inhibitory concentration (MIC), was not defined as the concentration at which no growth was observed, but as the concentration at which the lag time was infinite. The concentrations at which μ = μopt/2, (Cu50), were in the range of 2·2–2·6 mmol kg−1. Radial growth rate of P. expansum and the reciprocal of the lag time were linearly correlated (r = 0·84). In contrast, in the range 0–4 mmol kg−1, an inhibition of growth of B. cinerea was observed whereas germination remained unaffected (i.e. the lag time was constant). In the range 4–8 mmol kg−1, the radial growth rate of B. cinerea was almost constant (c. 1 mm day−1), but germination was inhibited (i.e. the lag time was increased). Conclusions: The MIC values were 4·7 mmol kg−1 for P. expansum, 8·2 and 7·3 mmol kg−1 for B. cinerea strain BC1 and BC2, respectively, demonstrating that some isolates of these moulds are resistant to copper. Significance and Impact of the Study: Copper concentrations at 4 mmol kg−1 would be sufficient to control the development of these isolates, but the toxicity of copper should be extended to other isolates and evaluated in vineyards.

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Copper Resistance and Accumulation in the Zygomycete Mucor rouxii

Cited by 13 publications

References 0 publications

Discriminating microsatellites from Macrophomina phaseolina and their potential association to biological functions

Discriminating microsatellites from Macrophomina phaseolina and their potential association to biological functions

Enhanced Copper Adsorption and Morphological Alterations of Cells of Copper-Stressed Mucor Rouxii

Modelling the inhibitory effect of copper sulfate on the growth of Penicillium expansum and Botrytis cinerea

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