Copper promotes sheep pancreatic duct organoid growth by activation of an antioxidant protein 1-dependent MEK-ERK pathway

Liu, Miao; Yu, Wen; Jin, Jing; Ma, Mıngjun; Tie-zhu, AN; Nie, Yuzhe; Teng, Chun‐Bo

doi:10.1152/ajpcell.00509.2019

Cited by 8 publications

(13 citation statements)

References 46 publications

(52 reference statements)

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“…Liu et al have established sheep pancreatic duct organoids to investigate the role and mechanism of copper in the sheep pancreas [90]. They showed that moderate concentrations of copper promote sheep pancreatic duct organoids and detailed the molecular mechanism through which copper induces the cell proliferation in the organoids.…”

Section: Sheepmentioning

confidence: 99%

Organoids: a promising new in vitro platform in livestock and veterinary research

Kar

Wells

Ellen

et al. 2021

Vet Res

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Organoids are self-organizing, self-renewing three-dimensional cellular structures that resemble organs in structure and function. They can be derived from adult stem cells, embryonic stem cells, or induced pluripotent stem cells. They contain most of the relevant cell types with a topology and cell-to-cell interactions resembling that of the in vivo tissue. The widespread and increasing adoption of organoid-based technologies in human biomedical research is testament to their enormous potential in basic, translational- and applied-research. In a similar fashion there appear to be ample possibilities for research applications of organoids from livestock and companion animals. Furthermore, organoids as in vitro models offer a great possibility to reduce the use of experimental animals. Here, we provide an overview of studies on organoids in livestock and companion animal species, with focus on the methods developed for organoids from a variety of tissues/organs from various animal species and on the applications in veterinary research. Current limitations, and ongoing research to address these limitations, are discussed. Further, we elaborate on a number of fields of research in animal nutrition, host-microbe interactions, animal breeding and genomics, and animal biotechnology, in which organoids may have great potential as an in vitro research tool.

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Section: Sheepmentioning

confidence: 99%

Organoids: a promising new in vitro platform in livestock and veterinary research

Kar

Wells

Ellen

et al. 2021

Vet Res

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Section: Development Of Farm and Companion Animal Organoid Models: Hi...mentioning

confidence: 99%

“…Sheep pancreatic duct organoids were generated using pancreatic duct cells from healthy fetus ( 77 ). The organoid was cultured in Matrigel containing EGF, R-Spondin-1, FGF10, and Noggin in Wnt3a-conditioned medium ( 77 ), which is a similar system described in mouse ( 105 ). Furthermore, this study demonstrated an importance of an appropriate amount of copper supplementation to the medium in order to achieve formation and growth of sheep pancreatic duct organoids ( 77 ).…”

Section: Development Of Farm and Companion Animal Organoid Models: Hi...mentioning

confidence: 99%

“…The organoid was cultured in Matrigel containing EGF, R-Spondin-1, FGF10, and Noggin in Wnt3a-conditioned medium ( 77 ), which is a similar system described in mouse ( 105 ). Furthermore, this study demonstrated an importance of an appropriate amount of copper supplementation to the medium in order to achieve formation and growth of sheep pancreatic duct organoids ( 77 ). It is known that copper plays a pivotal role in maintaining normal physiology in mammalian cells, yet its optimal concentrations for tissue or organ development have not been determined.…”

Section: Development Of Farm and Companion Animal Organoid Models: Hi...mentioning

confidence: 99%

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Farm and Companion Animal Organoid Models in Translational Research: A Powerful Tool to Bridge the Gap Between Mice and Humans

Kawasaki

Goyama

Tachibana

et al. 2022

Front. Med. Technol.

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Animal organoid models derived from farm and companion animals have great potential to contribute to human health as a One Health initiative, which recognize a close inter-relationship among humans, animals and their shared environment and adopt multi-and trans-disciplinary approaches to optimize health outcomes. With recent advances in organoid technology, studies on farm and companion animal organoids have gained more attention in various fields including veterinary medicine, translational medicine and biomedical research. Not only is this because three-dimensional organoids possess unique characteristics from traditional two-dimensional cell cultures including their self-organizing and self-renewing properties and high structural and functional similarities to the originating tissue, but also because relative to conventional genetically modified or artificially induced murine models, companion animal organoids can provide an excellent model for spontaneously occurring diseases which resemble human diseases. These features of companion animal organoids offer a paradigm-shifting approach in biomedical research and improve translatability of in vitro studies to subsequent in vivo studies with spontaneously diseased animals while reducing the use of conventional animal models prior to human clinical trials. Farm animal organoids also could play an important role in investigations of the pathophysiology of zoonotic and reproductive diseases by contributing to public health and improving agricultural production. Here, we discuss a brief history of organoids and the most recent updates on farm and companion animal organoids, followed by discussion on their potential in public health, food security, and comparative medicine as One Health initiatives. We highlight recent evolution in the culturing of organoids and their integration with organ-on-a-chip systems to overcome current limitations in in vitro studies. We envision multidisciplinary work integrating organoid culture and organ-on-a-chip technology can contribute to improving both human and animal health.

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“…These include canine organoids from the skin ( Wiener et al, 2018 ), prostate gland ( Usui et al, 2017 ), urinary bladder ( Elbadawy et al, 2019 ), kidney ( Chen et al, 2019 ) and liver ( Nantasanti et al, 2015 ), as well as porcine organoids from the esophagus submucosal gland ( von Furstenberg et al, 2017 ), rectum ( Adegbola et al, 2017 ), and testicle ( Sakib et al, 2019 ; Vermeulen et al, 2019 ). It also includes those from the mammary gland, pancreatic duct, and endometrium in cattle ( Martignani et al, 2018 ), sheep ( Liu et al, 2020 ) and horse ( Thompson et al, 2020 ), respectively. Notably, no respiratory or lung organoids have been reported in animals.…”

Section: Organoids: the Culturable Mini-organs In Humans And Animalsmentioning

confidence: 99%

Harness Organoid Models for Virological Studies in Animals: A Cross-Species Perspective

et al. 2021

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Animal models and cell culture in vitro are primarily used in virus and antiviral immune research. Whereas the limitation of these models to recapitulate the viral pathogenesis in humans has been made well aware, it is imperative to introduce more efficient systems to validate emerging viruses in both domestic and wild animals. Organoids ascribe to representative miniatures of organs (i.e., mini-organs), which are derived from three-dimensional culture of stem cells under respective differential conditions mimicking endogenous organogenetic niches. Organoids have broadened virological studies in the human context, particularly in recent uses for COVID19 research. This review examines the status and potential for cross-species applied organotypic culture in validating emerging animal, particularly zoonotic, viruses in domestic and wild animals.

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Copper promotes sheep pancreatic duct organoid growth by activation of an antioxidant protein 1-dependent MEK-ERK pathway

Cited by 8 publications

References 46 publications

Organoids: a promising new in vitro platform in livestock and veterinary research

Organoids: a promising new in vitro platform in livestock and veterinary research

Farm and Companion Animal Organoid Models in Translational Research: A Powerful Tool to Bridge the Gap Between Mice and Humans

Harness Organoid Models for Virological Studies in Animals: A Cross-Species Perspective

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