Copper nanoparticles elevate regeneration capacity of (Ocimum basilicum L.) plant via somatic embryogenesis

Ibrahim, Ahmed S.; Fahmy, Alaa; Ahmed, Shreen S.

doi:10.1007/s11240-018-1489-3

Cited by 37 publications

(15 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…46 Where it is reported that inclusion of CuNPs (5 µM) significantly improves somatic embryogenesis and regeneration in comparison to the control treatment (0.1 µM CuSO 4 • 5H 2 O), confirming that use of copper as CuNPs is superior to CuSO 4 • 5H 2 O. 46 Also, addition of CuNPs in induction medium had also showed positive effect on growth indices of regenerated plants like plant height, growth index, quantity of internodes, quantity of shoots and reproduction coefficient of Mentha longifolia. 70 AgNPs are chemically more reactive than silver ions because of their higher surface area-to-mass ratio.…”

Section: Employment Of Nanoparticles To Improve Callus Induction and Regenerationmentioning

confidence: 99%

“…Supplementing copper in the form of CuNPs in tissue culture media showed superior results than CuSO 4 · 5H 2 O in terms of somatic embryogenesis and regeneration. 46 However, above optimum concentration of CuNPs in the culture media is toxic. 47 , 48 Callogenesis and regeneration of seeds explants of rice ( Oryza sativa L.) were significantly increased by fortification of Chu’s N 6 media with 10 mg/l CuO-NPs.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exploring potential of copper and silver nano particles to establish efficient callogenesis and regeneration system for wheat (Triticum aestivumL.)

et al. 2021

View full text Add to dashboard Cite

show abstract

Section: Employment Of Nanoparticles To Improve Callus Induction and Regenerationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploring potential of copper and silver nano particles to establish efficient callogenesis and regeneration system for wheat (Triticum aestivumL.)

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Copper ions at the physiological level are crucial for growth and plant survival [ 15 ]. A somewhat elevated level of copper ions in induction or regeneration media may positively influence somatic embryogenesis [ 16 , 17 ] or androgenesis [ 18 ]. However, overstepping bounds of toxic doses may imbalance the chloroplast redox state altering photosynthesis [ 19 – 21 ], and inducing oxidative stress [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture

Orłowska

2021

J of Biol Res-Thessaloniki

View full text Add to dashboard Cite

Background Somatic embryogenesis is a phenomenon carried out in an environment that generates abiotic stress. Thus, regenerants may differ from the source of explants at the morphological, genetic, and epigenetic levels. The DNA changes may be the outcome of induction media ingredients (i.e., copper and silver ions) and their concentrations and time of in vitro cultures. Results This study optimised the level of copper and silver ion concentration in culture media parallel with the induction medium longevity step towards obtaining barley regenerants via somatic embryogenesis with a minimum or maximum level of tissue culture-induced differences between the donor plant and its regenerants. The optimisation process is based on tissue culture-induced variation evaluated via the metAFLP approach for regenerants derived under varying in vitro tissue culture conditions and exploited by the Taguchi method. In the optimisation and verification experiments, various copper and silver ion concentrations and the different number of days differentiated the tested trials concerning the tissue culture-induced variation level, DNA demethylation, and de novo methylation, including symmetric (CG, CHG) and asymmetric (CHH) DNA sequence contexts. Verification of optimised conditions towards obtaining regenerants with minimum and maximum variability compared to donor plants proved useful. The main changes that discriminate optimised conditions belonged to DNA demethylation events with particular stress on CHG context. Conclusions The combination of tissue culture-induced variation evaluated for eight experimental trials and implementation of the Taguchi method allowed the optimisation of the in vitro tissue culture conditions towards the minimum and maximum differences between a source of tissue explants (donor plant) and its regenerants from somatic embryos. The tissue culture-induced variation characteristic is mostly affected by demethylation with preferences towards CHG sequence context.

show abstract

“…The use of nanomaterials in enhancing the plant rooting and its growth under in vitro conditions has become recently a promising tool such as silver nanoparticles (Thangavelu et al 2018;Saha and Gupta 2018;Jadczak et al 2019;Ha et al 2020;Tymoszuk•and Kulus 2020), iron nanoparticles (Mozafari et al 2018 and Khan et MAYADA K. SELIEM et al al. 2020), zinc oxide nanoparticles (Nalci et al 2019;Abdel Wahab et al 2020; Mazaheri-Tirani and Dayani 2020), cobalt nanoparticles (Ha et al 2020), gold nanoparticle (Jadczak et al 2019), copper oxide nanoparticles (Javed et al 2017 andNalci et al 2019), copper nanoparticles (Ibrahim et al 2019), nickel oxide nanoparticles (Pinto et al 2019), and nano-Se (Sotoodehnia-Korani et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Response of Phalaenopsis Orchid to Selenium and Bio-Nano-Selenium: In Vitro Rooting and Acclimatization

Seliem

Abdalla

El-Ramady

2020

EBSS

View full text Add to dashboard Cite

O RCHID plants are considered one of the most valuable and largest flowering plant families, which have a premium position in the global market of cut-flowers. Rooting of orchids is still the main real problem that obstructs its propagation, as roots are hardly to develop.So, Orchid needs to improve its in vitro propagation by enhancing its rooting development. The plant tissue culture of orchids is the most promising field that has been commercially used for getting of virus-free and high-quality rooted plants and representing around 70% of transplants production. In this study the shoots of in vitro plantlets were separated and cultured on half MS medium supplemented with nano-Se (0,10, 20, 30, 40 and 50 mg l-1) or corresponding doses of bulk type selenite; sodium selenite (0, 0.5, 1, 2, 4, 6, 8 and 10 mg l-1) in separates treatments with or without 0.5 mg l-1 NAA (α-naphthalene acetic acid). The two different sources of selenium were selected at mentioned concentrations to improve orchid rooting and acclimatization. Supplementation of half MS medium with 50 mg l-1 nano-Se + 0.5 mg l-1 NAA recorded the highest significant values of rooting percentage and root length as well. Whereas, half MS medium fortified with 40 mg l-1 nano-Se + 0.5 mg l-1 NAA gave the optimum number of roots. On the other hand, this enhancing effect on rooting decreased after augmentation the culture medium with 10 mg l-1 selenite. It could be concluded that in vitro rooting of Phalaenopsis orchid was improved by supplementation of nano-Se up to 50 mg l-1 in presence of 0.5 mg l-1 NAA consequence, acclimatization process was enhanced.

show abstract

Copper nanoparticles elevate regeneration capacity of (Ocimum basilicum L.) plant via somatic embryogenesis

Cited by 37 publications

References 35 publications

Exploring potential of copper and silver nano particles to establish efficient callogenesis and regeneration system for wheat (Triticum aestivumL.)

Exploring potential of copper and silver nano particles to establish efficient callogenesis and regeneration system for wheat (Triticum aestivumL.)

Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture

Response of Phalaenopsis Orchid to Selenium and Bio-Nano-Selenium: In Vitro Rooting and Acclimatization

Contact Info

Product

Resources

About