Copper metabolism leading to and following acute hepatitis in LEC rats

Suzuki, Kazuo; Kanno, Sanae; Misawa, Shogo; Aoki, Yasunobu

doi:10.1016/0300-483x(94)02927-m

Cited by 33 publications

(23 citation statements)

References 19 publications

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“…9,10) It is likely that Cu, which increases in advanced chronic hepatitis and small HCC, exists in one of these forms. Liaw et al found a reduction in the SOD level in HCC and concluded that the lower Cu and Zn content in the hepatic tissue and sera accounts for this change.…”

Section: Discussionmentioning

confidence: 99%

“…The HPLC method proposed by Suzuki et al is considered to be well suited for in the analysis of not only Cu-MT and Zn-MT, but also Cu,Zn-SOD. 9,10) In the present study, we used the method of Suzuki et al for the analysis of Cu and Zn in MT and SOD. 9,10) SOD exists in cells mainly in two forms Cu,Zn-SOD and Mn-SOD.…”

Section: Discussionmentioning

confidence: 99%

“…9,10) In the present study, we used the method of Suzuki et al for the analysis of Cu and Zn in MT and SOD. 9,10) SOD exists in cells mainly in two forms Cu,Zn-SOD and Mn-SOD. In this study, no significant difference was noted between the contents of Cu and Zn for SOD of the normal hepatic tissue and tissue from patients with chronic liver diseases.…”

Section: Discussionmentioning

confidence: 99%

“…The remaining tissue was frozen rapidly and preserved at Ϫ80°C. According to the method by Suzuki et al, 9,10) each sample (weighing 314 to 643 mg) was placed in 3 volumes of buffer (100 mM Tri-HCl [pH 7.4] 0.25 M glucose), chilled in argon gas, homogenized for one minute using a Polytron Homogenizer (Kinematica, Lucerne, Swizerland), and centrifuged for 60 min at 170000 g at 2°C. During HPLC (Nihon Bunko Tosoh, Tokyo, Japan; column, TSK gel G3000 SW 7.5 mmϫ60 mm; buffer, 19 mM Tris-HCl [pH 8.0] 0.1% NaN 2 ) the supernatant was separated for fractions taken every 30 s. The metal content of each fraction was quantified using a desktop-type inductively coupled plasma (ICP) emission spectrochemical analyzer (Seiko SPS7000A, Tokyo, Japan).…”

Section: Subjectsmentioning

confidence: 99%

“…They reported that the Cu-MT content increased with a rise in the Cu level. 9,10) It has also been reported that in chronic liver diseases, the Cu concentration in the liver parenchyma increases with the progression of disease. 11,12) Tashiro-Itoh et al, in their study on human tissue bearing hepatocellular carcinoma (HCC), reported that the levels of both Cu and MT were increased in well-differentiated cancer but were lower in poorly differentiated cancer, which is in contrast with results from pericancerous hepatic tissue.…”

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confidence: 96%

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Evaluation of Distribution Patterns for Copper and Zinc in Metallothionein and Superoxide Dismutase in Chronic Liver Diseases and Hepatocellular Carcinoma Using High-Performance Liquid Chromatography (HPLC)

Kubo

Fukuda

Ebara

et al. 2005

Biological & Pharmaceutical Bulletin

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Metallothionein (MT) was initially discovered from a horse kidney in 1957 as a protein that contained cadmium. Metallothionein is a heat-stable, metal-binding protein with a molecular weight of 6500 to 7000. 1) A number of physiological and biological roles have been suggested for MT, including detoxification of heavy metals, metabolic regulation of Zinc (Zn) and Copper (Cu) (two trace elements that are essential to the body), and a radical-scavenging action that is closely related to the biological defense mechanism.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Subjectsmentioning

confidence: 99%

mentioning

confidence: 96%

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Evaluation of Distribution Patterns for Copper and Zinc in Metallothionein and Superoxide Dismutase in Chronic Liver Diseases and Hepatocellular Carcinoma Using High-Performance Liquid Chromatography (HPLC)

Kubo

Fukuda

Ebara

et al. 2005

Biological & Pharmaceutical Bulletin

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Rapid and reliable diagnosis of Wilson disease using X‐ray fluorescence

Kaščáková

Kewish

Rouzière

et al. 2016

The Journal of Pathology CR

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Wilson's disease (WD) is a rare autosomal recessive disease due to mutations of the gene encoding the copper‐transporter ATP7B. The diagnosis is hampered by the variability of symptoms induced by copper accumulation, the inconstancy of the pathognomonic signs and the absence of a reliable diagnostic test. We investigated the diagnostic potential of X‐ray fluorescence (XRF) that allows quantitative analysis of multiple elements. Studies were performed on animal models using Wistar rats (n = 10) and Long Evans Cinnamon (LEC) rats (n = 11), and on human samples including normal livers (n = 10), alcohol cirrhosis (n = 8), haemochromatosis (n = 10), cholestasis (n = 6) and WD (n = 22). XRF experiments were first performed using synchrotron radiation to address the elemental composition at the cellular level. High‐resolution mapping of tissue sections allowed measurement of the intensity and the distribution of copper, iron and zinc while preserving the morphology. Investigations were further conducted using a laboratory X‐ray source for irradiating whole pieces of tissue. The sensitivity of XRF was highlighted by the discrimination of LEC rats from wild type even under a regimen using copper deficient food. XRF on whole formalin‐fixed paraffin embedded needle biopsies allowed profiling of the elements in a few minutes. The intensity of copper related to iron and zinc significantly discriminated WD from other genetic or chronic liver diseases with 97.6% specificity and 100% sensitivity. This study established a definite diagnosis of Wilson's disease based on XRF. This rapid and versatile method can be easily implemented in a clinical setting.

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Macro–micro elementary content of liver and lungs in rats with alimentary adiposity (SRXRF)

Трунова¹,

Зверева²,

Churin³

et al. 2009

X-Ray Spectrometry

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In alimentary adiposity, some microelements take active part in the metabolism change that proves to be true by an essential deviation from the norm of their concentration in liver and lung tissues during the physiological hunger and soon after fat consumption. The concentrations (in µg/g) of the following chemical elements P, S, Cl, K, Ca, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, Br, Rb, and Sr were determined by synchrotron radiation excited X-ray fluorescence (SRXRF) analysis. The measurements were carried out at the experimental station of X-ray fluorescence elemental analysis (Budker Institute of Nuclear Physics SB RAS, storage ring VEPP-3). In alimentary adiposity, deficiency of separate microelements does not concern all organisms, but only some organs. At the condition of physiological hunger in the liver and lungs of rats with alimentary adiposity, the concentrations of As and Br are decreased; besides, in lungs the concentrations of Zn, Se, and Rb are also lowered. Copyright

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Copper metabolism leading to and following acute hepatitis in LEC rats

Cited by 33 publications

References 19 publications

Evaluation of Distribution Patterns for Copper and Zinc in Metallothionein and Superoxide Dismutase in Chronic Liver Diseases and Hepatocellular Carcinoma Using High-Performance Liquid Chromatography (HPLC)

Evaluation of Distribution Patterns for Copper and Zinc in Metallothionein and Superoxide Dismutase in Chronic Liver Diseases and Hepatocellular Carcinoma Using High-Performance Liquid Chromatography (HPLC)

Rapid and reliable diagnosis of Wilson disease using X‐ray fluorescence

Macro–micro elementary content of liver and lungs in rats with alimentary adiposity (SRXRF)

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