Copper-induced oxidative stress in rainbow trout gill cells

Bopp, Stephanie K.; Abicht, Helge K.; Knauer, Katja

doi:10.1016/j.aquatox.2007.10.014

Cited by 157 publications

(59 citation statements)

References 42 publications

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“…Previous studies have shown that in response to . Previous studies have shown that in response to metal exposure, there is an increase in the formation of oxygen free radicals or reactive oxygen species (ROS) in rats, rainbow trout and M. rosenbergii (Stohs and Bagchi, 1995;Bopp et al, 2008); this can result in widespread damage to cells because of lipid peroxidation and genotoxicity. Respiratory burst, the release of reactive oxygen species (ROS) by haemocytes, is a critical step in the innate immune response.…”

Section: Discussionmentioning

confidence: 99%

Toxic effects of heavy metals (cadmium plus mercury) on haematological parameters and DNA damage in Lates calcarifer

Senthamilselvan¹

2012

J. Toxicol. Environ. Health Sci.

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The present study was carried out to evaluate the effects of toxic metals (cadmium plus mercury) on the haematology and DNA damage of fish, Lates calcarifer for every 24 h up to 96 h. A significant gradual decrease of haemoglobin (-35.01 to -24.11) and hematocrit (-35.01 to -31.78) was noted at acute concentration of cadmium plus mercury (3.0 ppm) as compared to that of control. The comet assay of fish blood cells exhibited a significant higher DNA damage and the highest OTM was observed in 96 h of acute mixed metals concentration. From this investigation, the results proved that the combined metal toxicity induced haematological and DNA damage of fish Lates calcarifer than individual metal toxicity.

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Section: Discussionmentioning

confidence: 99%

Toxic effects of heavy metals (cadmium plus mercury) on haematological parameters and DNA damage in Lates calcarifer

Senthamilselvan¹

2012

J. Toxicol. Environ. Health Sci.

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“…According to Lee et al [36] this cell line allows the detailed study of both the cytotoxicity and biotransformation of chemicals that cause cytotoxicity, with a greater efficiency than an in vivo study of the gills. RTgill-W1 cells were used to evaluate the toxicity of industrial waste water, polycyclic aromatic hydrocarbons and heavy metals [37][38][39][40][41][42]. The lower sensitivity of the fish cells toward microcystins (compared to the mammalian cells) in our study could be explained with the absence of detectable mRNA levels of organic anion transporter polypeptides [43].…”

Section: Discussionmentioning

confidence: 66%

In vitro and in vivo toxicity evaluation of the freshwater cyanobacterium Heteroleiblenia kuetzingii

et al. 2013

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Cyanobacteria are prokaryotic organisms characterized by their ability to produce secondary metabolites with different biological activities. The aim of this work was to evaluate the in vitro and in vivo toxicity of the cosmopolitan freshwater cyanobacterium H. kuetzingii. An extract from H. kuetzingii and cyanobacterial growth media were assessed for presence of intracellular and extracellular toxins by in vitro tests using primary cell cultures from mouse kidney and fibroblasts, cell lines A549 and 3T3, a fish cell line RTgill-W1 as well as by a traditional in vivo mouse bioassay. The presence of toxicity was compared with the ELISA and HPLC data for corresponding cyanotoxins. In vitro tests showed pronounced cytotoxicity of the cyanobacterium extract and growth medium in which H. kuetzingii released potential extracellular toxic compounds as the mammalian cells were significantly more sensitive to exposure compared to the fish cells. Histopathological analyses of the liver and kidneys of treated mice showed pathological changes such as leukocyte infiltration and necrosis, changes in the proximal and distal convoluted tubules, lack of differentiation of Bowman's space, enlarged Bowman's capsules and massive hemorrhages. ELISA and HPLC analyses confirmed the presence of saxitoxins and microcystins at low concentrations. In addition, the histological analyses suggest that H. kuetzingii produces other, yet unknown toxic metabolites. Monitoring efforts are therefore required to evaluate the potential hazard for the freshwater aquatic systems and possible public health implications associated with this cyanobacterium.

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“…As many pesticides, diuron and copper may generate reactive oxygen species (ROS) and induce an overwhelming of the antioxidant system (Dautremepuits et al, 2004;Luna-Acosta et al, 2012). This oxidative stress could be accountable to reduction of cellular viability by increasing DNA strand breaks leading to apoptosis (Bopp et al, 2008) or decreasing functional integrity of the lysosomal membrane (Roméo et al, 2000;Krumschnabel et al, 2005) inducing necrosis by release of acid hydrolases (Holtzman, 1989). As discussed above, increase of cellular mortality could induce the observed impairment of phagocytosis capacity.…”

Section: Discussionmentioning

confidence: 99%

Involvement of fish immunomarkers in environmental biomonitoring approach: Urban and agri-viticultural context

Bado‐Nilles

Jolly

Lamand

et al. 2015

Ecotoxicology and Environmental Safety

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The Champagne region (France) is characterized by various chemical environmental pressures which could interfere with the immune status of natural populations of European bullhead, Cottus sp. Some adult fish were caught by electrofishing in spring, summer and autumn to determined immune effect of urban (Muizon), intensive agricultural (Bouy; Prunay) or viticultural (Serzy; Prunay)influences. The major results demonstrated an increase of cellular mortality and a decrease of phagocytosis activity in the stations impacted by agri-viticultural chemicals.These immunomodulations followed the temporal variability due to different treatments (agricultural impacts on spring; viticultural effects on autumn). At the present time, not enough data was provided to confirm the impact of agri-viticultural chemicals on fish immune system without interaction with other environmental factors. For example, in summer, the immunomarkers seems to be not only correlated with water contamination but also with other environmental factors (pathogens, physical field degradation, nutrients, temperature ...). Nevertheless, immune parameters give a global view of organism and ecosystem health explaining growing interest for these biomarkers in environmental risk assessment.

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Copper-induced oxidative stress in rainbow trout gill cells

Cited by 157 publications

References 42 publications

Toxic effects of heavy metals (cadmium plus mercury) on haematological parameters and DNA damage in Lates calcarifer

Toxic effects of heavy metals (cadmium plus mercury) on haematological parameters and DNA damage in Lates calcarifer

In vitro and in vivo toxicity evaluation of the freshwater cyanobacterium Heteroleiblenia kuetzingii

Involvement of fish immunomarkers in environmental biomonitoring approach: Urban and agri-viticultural context

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