Copper-Induced Modifications of the Trophic Relations in Riverine Algal–bacterial Biofilms

Barranguet, Christiane; Ende, Frank P. van den; Rutgers, Michiel; Breure, A.M.; Greijdanus, Marianne; Sinke, J.J.; Admiraal, Wim

doi:10.1897/1551-5028(2003)022<1340:cimott>2.0.co;2

Cited by 31 publications

(47 citation statements)

References 30 publications

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“…It is widely used in the industrial and agricultural sectors, but it is also highly toxic to organisms, such as algae, fungi and many bacteria and viruses (Merian 1991). High concentrations of copper in drinking water are hazardous to human health (Barranguet et al 2003). Some cases of liver damage of children have been proved to be associated with the excessive intake of copper (Zietz et al 2003).…”

Section: Hgmentioning

confidence: 99%

Detection of heavy metals (Cu+2, Hg+2) by biosynthesized silver nanoparticles

2015

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Here, we are reporting two methods for detection of Cu ?2 ion and Hg ?2 ions using biosynthesized silver nanoparticles. The detection of Cu ?2 ion was based on changes in absorbance resulting from complex formation of the metal ion. Various concentrations of Cu ?2 ion were used to test the linearity and sensitivity of the method. A new peak at around 770 nm, in addition to the peak of the AgNP at 406 nm, was observed in each case (above 20 lM). With the increase of concentration of Cu ?2 ion solution, the absorbance at 406 nm peak decreased and that of 770 nm increased gradually. The calibration curve obtained from the ratio of the absorption coefficients of these two peaks (Ex 770/406 ) versus concentration of Cu ?2 ions enables one to estimate quantitatively the amount of Cu ?2 ions present in water in lM levels. This AgNP was further functionalized with 3-mercapto-1, 2-propanediol (MPD) for detection of Hg ?2 present in water by colorimetric method. As soon as Hg ?2 solution was added in MPDfunctionalized AgNP (MPD-AgNP), a new peak at around 606 nm appeared along with the peak at 404 nm. The new peak might be due to the aggregations occurred by the recognition of heavy metal ion Hg ?2 by MPD-AgNP through dipropionate ion. A calibration curve between the ratios of the absorption coefficients of these two peaks (Ex 404/606 ) and concentration of Hg ?2 was drawn for quantitative estimation of Hg ?2 present in water at lM level.

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Section: Hgmentioning

confidence: 99%

Detection of heavy metals (Cu+2, Hg+2) by biosynthesized silver nanoparticles

2015

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“…Among various environmental and physiologically important analytes copper is most studied element due to its significant impact on environment as well as in physiology of the various organisms [2]. It is found to be highly toxic to some organisms such as many bacteria and viruses [3]. Due to its toxicity to bacteria, higher levels of copper hamper the self-purification capability of the sea or rivers and destroy the biological reprocessing systems in water.…”

Section: Introductionmentioning

confidence: 99%

A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

Kempahanumakkagaari

Ramakrishnappa

Malingappa

2014

Journal of Luminescence

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a b s t r a c tA simple, sensitive and selective fluorescent chemo dosimeter rhodamine B phenyl hydrazide (RBPH) for Cu 2 þ was proposed. This probe is non fluorescent and colorless but exhibits fluorescent enhancement at 580 nm and displayed color change from colorless to pink for Cu 2 þ in the pH range 1-6. Fluorescence microscope experimental results reveals that this chemo sensor is cell permeable and can be used for fluorescence imaging of Cu 2 þ ions in living cells. This probe can detect Cu 2 þ with good linear relationships from 10 to 100 nM with r ¼0.99971 then limit of detection was found to be 0.015 nM with 7 0.91% RSD at 10 nM concentrations.

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“…However, the measured Cu effects on the photosystem were not related to changes occurring in the composition of the phototrophic community as observed by microscopy. Nevertheless, as suggested by Barranguet et al (4), changes in phototrophic community structure could lead to a modification in the type of organic compounds released by the phototrophic organisms, which in turn could induce a change in the heterotrophic community structure, as Watson and Bollen (37) demonstrated.…”

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“…Possible interactions between phototrophic and heterotrophic compartments of a biofilm may be disturbed when one compartment is severely affected by a stress factor, e.g., an increase in toxicant concentration. In a recent publication, Barranguet et al (4) reported preliminary results providing insights on the disturbance by Cu of the relationship between algae and bacteria of a biofilm. We extend the knowledge of this relationship by showing that Cu can affect the bacterial community both physiologically and structurally without an apparent link to disturbance of the phototrophic community.…”

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Analysis of Structural and Physiological Profiles To Assess the Effects of Cu on Biofilm Microbial Communities

Massieux

Boivin

Ende

et al. 2004

Appl Environ Microbiol

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We investigated the effects of copper on the structure and physiology of freshwater biofilm microbial communities. For this purpose, biofilms that were grown during 4 weeks in a shallow, slightly polluted ditch were exposed, in aquaria in our laboratory, to a range of copper concentrations (0, 1, 3, and 10 M). Denaturing gradient gel electrophoresis (DGGE) revealed changes in the bacterial community in all aquaria. The extent of change was related to the concentration of copper applied, indicating that copper directly or indirectly caused the effects. Concomitantly with these changes in structure, changes in the metabolic potential of the heterotrophic bacterial community were apparent from changes in substrate use profiles as assessed on Biolog plates. The structure of the phototrophic community also changed during the experiment, as observed by microscopic analysis in combination with DGGE analysis of eukaryotic microorganisms and cyanobacteria. However, the extent of community change, as observed by DGGE, was not significantly greater in the copper treatments than in the control. Yet microscopic analysis showed a development toward a greater proportion of cyanobacteria in the treatments with the highest copper concentrations. Furthermore, copper did affect the physiology of the phototrophic community, as evidenced by the fact that a decrease in photosynthetic capacity was detected in the treatment with the highest copper concentration. Therefore, we conclude that copper affected the physiology of the biofilm and had an effect on the structure of the communities composing this biofilm.Copper has been applied as an algaecide for many years, e.g., in antifouling ship paints, and has been used in the composition of many materials, such as porcelains and bricks, which for a long time were simply deposited in dumping grounds when no longer in use, increasing the load of cupric compounds in soils and waters, and leading to concerns about their toxicity. However, our knowledge of the effects of this metal on the structure and function of microbial communities in freshwater is still limited. Several detrimental effects of Cu have been reported to occur in pure cultures of phototrophic species. These effects include depression of photosynthesis and respiration, inhibition of cell division, and cell death (15). Aquatic microorganisms, phytoplankton and bacteria, often live in communities, forming organized assemblages at the surfaces of rocks, sediment, and submerged plants. These epilithic, epiphytic, or epipelon assemblages are generally described as biofilms (30). Barranguet et al., studying heavy metal effects on aquatic biofilms, showed that Cu affects the physiology of phototrophic organisms (1), that it accumulates in phototrophic biofilms proportionally to the concentration of exposure, and that some algal species react morphologically to an increased Cu concentration (3). However, the measured Cu effects on the photosystem were not related to changes occurring in the composition of the phototrophic community as...

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Copper-Induced Modifications of the Trophic Relations in Riverine Algal–bacterial Biofilms

Cited by 31 publications

References 30 publications

Detection of heavy metals (Cu+2, Hg+2) by biosynthesized silver nanoparticles

Detection of heavy metals (Cu+2, Hg+2) by biosynthesized silver nanoparticles

A new rhodamine B based fluorometric chemodosimeter for Cu2+ ion in aqueous and cellular media

Analysis of Structural and Physiological Profiles To Assess the Effects of Cu on Biofilm Microbial Communities

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