Copper(II) thiosemicarbazonate molecular modifications modulate apoptotic and oxidative effects on U937 cell line

Bisceglie, Franco; Pinelli, Silvana; Alinovi, Rossella; Tarasconi, Pieralberto; Buschini, Annamaria; Mussi, Francesca; Mutti, Antonio; Pelosi, Giorgio

doi:10.1016/j.jinorgbio.2012.07.006

Cited by 23 publications

(22 citation statements)

References 31 publications

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“…Lipid peroxidation was evaluated by ‘‘Thiobarbituric Acid Reactive Substances” (TBARS) method, as previously described [ 28 ], using Malondialdehyde as a standard for the calibration curve (range 0–10 μM). In preliminary experiments, we documented that cells treated with H 2 O 2 50μM for 30 min displayed substantial increases (about 2-fold) in TBARS when compared to untreated cells, thereby validating the use of TBARS as a “footprint” of increased oxidative stress in our experimental setting..…”

Section: Characterization Of Tio 2 and Co mentioning

confidence: 99%

Effects of TiO2 and Co3O4 Nanoparticles on Circulating Angiogenic Cells

et al. 2015

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Background and AimSparse evidence suggests a possible link between exposure to airborne nanoparticles (NPs) and cardiovascular (CV) risk, perhaps through mechanisms involving oxidative stress and inflammation. We assessed the effects of TiO2 and Co3O4 NPs in human circulating angiogenic cells (CACs), which take part in vascular endothelium repair/replacement.MethodsCACs were isolated from healthy donors’ buffy coats after culturing lymphomonocytes on fibronectin-coated dishes in endothelial medium for 7 days. CACs were pre-incubated with increasing concentration of TiO2 and Co3O4 (from 1 to 100 μg/ml) to test the effects of NP – characterized by Transmission Electron Microscopy – on CAC viability, apoptosis (caspase 3/7 activation), function (fibronectin adhesion assay), oxidative stress and inflammatory cytokine gene expression.ResultsNeither oxidative stress nor cell death were associated with exposure to TiO2 NP (except at the highest concentration tested), which, however, induced a higher pro-inflammatory effect compared to Co3O4 NPs (p<0.01). Exposure to Co3O4 NPs significantly reduced cell viability (p<0.01) and increased caspase activity (p<0.01), lipid peroxidation end-products (p<0.05) and pro-inflammatory cytokine gene expression (p<0.05 or lower). Notably, CAC functional activity was impaired after exposure to both TiO2 (p<0.05 or lower) and Co3O4 (p<0.01) NPs.ConclusionsIn vitro exposure to TiO2 and Co3O4 NPs exerts detrimental effects on CAC viability and function, possibly mediated by accelerated apoptosis, increased oxidant stress (Co3O4 NPs only) and enhancement of inflammatory pathways (both TiO2 and Co3O4 NPs). Such adverse effects may be relevant for a potential role of exposure to TiO2 and Co3O4 NPs in enhancing CV risk in humans.

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Section: Characterization Of Tio 2 and Co mentioning

confidence: 99%

Effects of TiO2 and Co3O4 Nanoparticles on Circulating Angiogenic Cells

et al. 2015

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“…Thiosemicarbazones (TSCs) are sulfur-containing organic substances whose biological properties have been extensively studied [1][2][3][4][5][6][7][8], in particular their activities against microbial diseases as malaria, small-pot, influenza, leishmaniasis or chagas [9][10][11][12][13][14], neurological pathologies [15,16] and cancer [17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36]. Different biological targets have been identified, like DNA [37][38][39], RNA [40] and several enzymes as RNA-dependent DNA polymerases [41], xanthine oxidoreductase [42], thioredoxin reductase [43,44], topoisomerase IIa [45][46][47] or s...…”

Section: -Introductionmentioning

confidence: 99%

Revisiting the thiosemicarbazonecopper(II) reaction with glutathione. Activity against colorectal carcinoma cell lines

García–Tojal

Gil‐García

Fouz

et al. 2018

Journal of Inorganic Biochemistry

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Thiosemicarbazones (TSCs), and their copper derivatives, have been extensively studied mainly due to the potential applications as antitumor compounds. A part of the biological activity of the TSC-Cu complexes rests on their reactivity against cell reductants, as glutathione (GSH). The present paper describes the structure of the [Cu(PTSC)(ONO)] compound (1) (HPTSC=pyridine-2-carbaldehyde thiosemicarbazone) and its spectroscopic and magnetic properties. ESI studies performed on the reaction of GSH with 1 and the analogous [{Cu(PTSC*)(ONO)}] derivative (2, HPTSC*=pyridine-2-carbaldehyde 4N-methylthiosemicarbazone) show the absence of peaks related with TSC-Cu-GSH species. However GSH-Cu ones are detected, in good agreement with the release of Cu ions after reduction in the experimental conditions. The reactivity of 1 and 2 with cytochrome c and myoglobin and their activities against HT-29 and SW-480 colon carcinoma cell lines are compared with those shown by the free HPTSC and HPTSC* ligands.

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“…From this perspective, hydrazones seem promising, because of their well-known coordinating abilities and biological properties [4][5][6][7][8][9][10][11][13][14][15][16]. We have focused our attention on the o-vanillin acylhydrazone scaffold: the potentially tetradentate ligand H 2 L shows a great versatility.…”

Section: Discussionmentioning

confidence: 99%

“…Acylhydrazone-based ligands and their transition metal complexes are widely studied in catalysis [1], as building blocks in supramolecular chemistry [2,3], as anticancer agents [4,5], and, particularly interesting for our research, antiviral molecules [6,7]. They are often used to build multinuclear metal complexes since they are versatile ligands and their coordination properties can be easily tuned from an electronic and a steric point of view [8][9][10].…”

Section: Introductionmentioning

confidence: 99%