Copper(II) Oxide Nanoflakes Based Impedimetric Immunosensor for Label Free Determination of Cancer Antigen-125

Raghav, Ragini; Srivastava, Sudha

doi:10.1166/sl.2016.3591

Cited by 9 publications

(4 citation statements)

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“…Later, using the same concept as studied in ref , EIS immunosensors based on copper(II) oxide nanoflake (CuO(II)) modified gold screen-printed electrodes (CuO(II)/gold SPE) were developed . The label-free biosensor was fabricated by immobilizing the anti-CA125 antibody onto amine-functionalized CuO(II) nanoflakes.…”

Section: Ec Biosensing Of Oncomarkersmentioning

confidence: 99%

“…Hence, further investigation concerning these aspects is crucial for its clinical application. Table summarizes the results of the reported research in the development of EC imunosensors for CA125 biomarker determination from 2015 to mid-2022. ,,,− ,− …”

Section: Ec Biosensing Of Oncomarkersmentioning

confidence: 99%

“…Later, using the same concept as studied in ref 107, EIS immunosensors based on copper(II) oxide nanoflake (CuO-(II)) modified gold screen-printed electrodes (CuO(II)/gold SPE) were developed. 108 The label-free biosensor was fabricated by immobilizing the anti-CA125 antibody onto amine-functionalized CuO(II) nanoflakes. In this study, the effect of temperature on the immunocomplex formation was investigated at the optimum PBS of pH 7.4, which depicted a linearly increasing impedimetric response from 1.0 to 100.0 IU/mL with LOD of 0.77 IU/mL and a dynamic range of 0.77−500.0 IU/mL.…”

Section: Ec Biosensing Of Oncomarkersmentioning

confidence: 99%

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Biomarkers for Early Diagnosis of Ovarian Carcinoma

Manasa¹,

Mascarenhas²,

Shetti

et al. 2022

ACS Biomater. Sci. Eng.

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The leading cause of gynecological cancer-related morbidity and mortality is ovarian cancer (OC), which is dubbed a silent killer. Currently, OC is a target of intense biomarker research, because it is often not discovered until the disease is advanced. The goal of OC research is to develop effective tests using biomarkers that can detect the disease at the earliest stages, which would eventually decrease the mortality, thereby preventing recurrence. Therefore, there is a pressing need to revisit the existing biomarkers to recognize the potential biomarkers that can lead to efficient predictors for the OC diagnosis. This Perspective covers an update on the currently available biomarkers used in the triaging of OC to gain certain insights into the potential role of these biomarkers and their estimation that are crucial to the understanding of neoplasm progression, diagnostics, and therapy.

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Section: Ec Biosensing Of Oncomarkersmentioning

confidence: 99%

Section: Ec Biosensing Of Oncomarkersmentioning

confidence: 99%

Section: Ec Biosensing Of Oncomarkersmentioning

confidence: 99%

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Biomarkers for Early Diagnosis of Ovarian Carcinoma

Manasa¹,

Mascarenhas²,

Shetti

et al. 2022

ACS Biomater. Sci. Eng.

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“…The electrochemical immunosensor was characterized by electrochemical impedance spectroscopy (EIS) using [Fe(CN) 6 ] 3−/4− in solution as the redox probe and the limit of detection was 0.25 pg/mL [31]. Raghav [32] and collaborators reported a copper(II) oxide nanoflakes based impedimetric immunosensor for detection of cancer antigen 125 with a lower limit of detection (0.77 IU/ mL). Nguyen and collaborators [33] developed a SERS immunosensor built-in microfluidic system based on Rhodamine-6G (R6G)-conjugated immunogolds with limit of detection of 15fM.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Electrochemical immunosensor based on ZnO nanorods-Au nanoparticles nanohybrids for ovarian cancer antigen CA-125 detection

Gasparotto

Costa

et al. 2017

Materials Science and Engineering: C

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In this research, ZnO nanorods - Au nanoparticles nanohybrids have been fabricated and employed to sensitive electrochemical strategy for the specific detection of the ovarian cancer antigen CA-125/MUC126. The microdevice was developed in our lab based on gold and silver electrodes sputtered on glass substrate. The ZnO nanorods arrays were grown on working electrode using assisted microwave hydrothermal synthesis than gold nanoparticles (Au NPs) were deposited by sputtering. The Au NPs onto ZnO nanorods surface provides a favorable platform for efficient loading of anti-CA-125 antibody via binding with cystamine and glutaraldehyde. The effective loading of the biological material (CA-125 antibody and antigen) on the matrix was observed by SEM images. The electrochemical immunosensor shows a sensitive response to ovarian cancer antigen recombinant human CA-125/MUC126 with detection of 2.5ng/μL, 100 times lower than immunoblot system. Due to high specificity, reproducibility and noteworthy stability, the developed sensor will provide a sensitive, selective and convenient approach to be used to detect CA-125/MUC126.

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