Copper(II) and zinc(II) complexes of the peptides Ac-HisValHis-NH 2 and Ac-HisValGlyAsp-NH 2 related to the active site of the enzyme CuZnSOD

Bóka, Beáta; Myari, Alexandra; Sóvágó, Imre; Hadjiliadis, Nick

doi:10.1016/j.jinorgbio.2003.09.012

Cited by 78 publications

(50 citation statements)

References 38 publications

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“…The determined pK values (see Table 1) representing the deprotonation processes are macroscopic constants and cannot be assigned to individual histidine residues. The pK values are very similar to those measured for other terminally protected short Hisrich peptides [41,42,[48][49][50][51][52], which reflect no specific interaction between the deprotonating groups of the ligands.…”

Section: Protonation Equilibria Of the Ligandssupporting

confidence: 74%

“…8a). Similar cooperativity was attributed to the copper(II) promoted amide deprotonations of several peptides containing the His-X-His motif (X -Pro) [40,50,52,59]. In contrast with HP1, HP2 does contain a HGH(H) segment in its central part that could offer an {N im , N À amide , N À amide , N im } type coordination for copper(II).…”

Section: Interaction Of Ac-hhphg-nh 2 and Ac-hhphghhphg-nh 2 With Copmentioning

confidence: 76%

“…These results are discussed below, together with those of HP2. Titrations could be performed only up to pH $ 7.1 since above this pH precipitation occurred in parallel with the deprotonation process leading to ZnH -1 L, similarly to several other systems of N-terminally protected oligopeptides and zinc(II) [39,41,42,[48][49][50].…”

Section: Interaction Of Ac-hhphg-nh 2 and Ac-hhphghhphg-nh 2 With Zinmentioning

confidence: 99%

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Probing the Cu2+ and Zn2+ binding affinity of histidine-rich glycoprotein

Jancsó

Kolozsi

Gyurcsik

et al. 2009

Journal of Inorganic Biochemistry

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Section: Protonation Equilibria Of the Ligandssupporting

confidence: 74%

Section: Interaction Of Ac-hhphg-nh 2 and Ac-hhphghhphg-nh 2 With Copmentioning

confidence: 76%

Section: Interaction Of Ac-hhphg-nh 2 and Ac-hhphghhphg-nh 2 With Zinmentioning

confidence: 99%

See 1 more Smart Citation

Probing the Cu2+ and Zn2+ binding affinity of histidine-rich glycoprotein

Jancsó

Kolozsi

Gyurcsik

et al. 2009

Journal of Inorganic Biochemistry

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“…According to Boka et al, copper and zinc binds to the nitrogen (C=N) in the imidazole ring of histidine in the range of 250 -300 nm giving rise to a strong sharp peak with high absorbance. However, it was also mentioned that copper also binds to the carboxylate side chain of aspartic acid and henceforth, the values are shifted further right (higher wavelength) due to the acidic nature of the metallopeptides [7], as observed in this study. …”

Section: Uv-visible Spectral Analysis One Of the Distinctive Featuressupporting

confidence: 66%

“…Just like the UV-vis spectra, a strong signal was observed in the range of 285 -340 nm (Figure 2). This was due to the presence of the imidazole ring of histidine [7]. In some of the metallopeptides, quenching was observed in this range.…”

Section: Uv-fluorescence Spectral Analysismentioning

confidence: 95%

Spectroscopic Characterization of Copper(ii)-Based Tetrapeptides

Rahman¹,

Begum²,

Tahir³

et al. 2016

MJAS

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Five different histidine and aspartic acid based tetrapeptides were designed using LOMETS and PyMol. They were chemically synthesized following the solid phase Fmoc-peptide synthesis protocols and were analysed using the reverse-phase High Performance Liquid Chromatography (HPLC) C 18 analytical column for the purity. The peptides were further analysed by Liquid Chromatography Mass Spectrometry (LCMS) to see if the desired peptides were synthesized systematically. Copper(II) acetate monohydrate was bound to the peptides and the best molar ratio for the binding of these metal salts to peptides was 2:1. These observations were monitored through several spectroscopic techniques. The first physical observations for the successful synthesis of metallopeptides were the colour change, the melting/decomposition points and the solubility of these metallopeptides. Due to the visible colour change of the peptides to metallopeptides, UV-Visible spectroscopy and UVFluorescence spectroscopy were used as a qualitative analysis tests and the results were in agreement with other researchers' data from similar researches.Keywords: copper(II), histidine, aspartic acid, tetrapeptides, spectroscopic Abstrak Lima tetrapeptida berbeza yang mempunyai histidine dan asid aspartik direkabentuk oleh LOMETS dan PyMol. Tetrapeptida ini disintesis mengikut skim fasa pepejal peptida dan dianalisis menggunakan fasa sonsang Kromatografi Cecair Berprestasi Tinggi (HPLC) C 18 turus analisis untuk mengetahui keaslian peptida. Kesemua peptida ini dianalisis mengunakan Kromatografi CecairSpektrometer Jisim (LC-MS) untuk mengkaji sintesis peptida secara sistematik. Kuprum(II) asetat monohidrat terikat kepada peptida dan nisbah mol yang terbaik untuk mengikat garam logam ini kepada peptida adalah 2:1. Cerapan ini telah dipantau melalui beberapa teknik spektroskopi. Pemerhatian fizikal pertama untuk logam peptida yang berjaya disintesis ialah perubahan warna, takat lebur/penguraian dan kebolehlarutan peptida logam ini. Oleh kerana perubahan warna yang nyata dalam dari peptida kepada logam peptida, spektroskopi Ultralembayung-tampak (UV-Vis) dan spektroskopi Ultralembayung-Pendafluor (UVFluorescence) digunakan sebagai ujian analisis kuantatif dan data yang diperolehi adalah setara dengan perolehan data bagi penyelidikan yang serupa.Kata kunci: Kuprum(II), histidin, asid aspartik, tetrapeptida, spektroskopi ISSN -2506 Mohd. Basyaruddin et al: SPECTROSCOPIC CHARACTERIZATION OF COPPER(II)-BASED TETRAPEPTIDES 736Introduction Metallopeptides that are easier and cheaper to synthesise when compared to metalloproteins have caught the attention of many researchers. Metallopeptides, synthesized from metal salt and peptides, are known as pseudoproteins. They imitate the applications of large proteins especially in the industrial field of catalysis as well as in biological applications [1]. Metallopeptides are hybrid structures that combine peptides and metal and in this project, the transition metal copper(II) was used, that has various roles when used as b...

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