Copper(II)−Alizarin Red S Complex as an Efficient Chemiluminescent Probe for the Detection of Human Serum Proteins after Polyacrylamide Gel Electrophoresis

Wang, Zhenzhen; Liu, Xia; Baeyens, Willy R. G.; Delanghe, Joris; Ouyang, Jin

doi:10.1021/pr800365n

Cited by 10 publications

(4 citation statements)

References 31 publications

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“…A method for electric transfer of analytes from the one-dimensional gel electrophoresis (1-DE) gel to a MALDI target plate has been developed . The gels may be stained with Coomasie brilliant blue, mono or diamine silver, nile red, zinc, or many novel reagents (Diezel, Kopperschlager, & Hofmann, 1972;Dionne et al, 1994;Fernandez-Patron et al, 1995;Wang et al, 2008e).…”

Section: E One-dimensional Poly Acrylamide Gel Electrophoresis (1d Pmentioning

confidence: 99%

Mass spectrometry of peptides and proteins from human blood

Zhu

Bowden

Zhang

et al. 2010

Mass Spectrometry Reviews

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It is difficult to convey the accelerating rate and growing importance of mass spectrometry applications to human blood proteins and peptides. Mass spectrometry can rapidly detect and identify the ionizable peptides from the proteins in a simple mixture and reveal many of their post-translational modifications. However, blood is a complex mixture that may contain many proteins first expressed in cells and tissues. The complete analysis of blood proteins is a daunting task that will rely on a wide range of disciplines from physics, chemistry, biochemistry, genetics, electromagnetic instrumentation, mathematics and computation. Therefore the comprehensive discovery and analysis of blood proteins will rank among the great technical challenges and require the cumulative sum of many of mankind's scientific achievements together. A variety of methods have been used to fractionate, analyze and identify proteins from blood, each yielding a small piece of the whole and throwing the great size of the task into sharp relief. The approaches attempted to date clearly indicate that enumerating the proteins and peptides of blood can be accomplished. There is no doubt that the mass spectrometry of blood will be crucial to the discovery and analysis of proteins, enzyme activities, and post-translational processes that underlay the mechanisms of disease. At present both discovery and quantification of proteins from blood are commonly reaching sensitivities of ∼1 ng/mL.

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Section: E One-dimensional Poly Acrylamide Gel Electrophoresis (1d Pmentioning

confidence: 99%

Mass spectrometry of peptides and proteins from human blood

Zhu

Bowden

Zhang

et al. 2010

Mass Spectrometry Reviews

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“…Alizarin red S with the chemical name of 1,2-dihydroxy-9,10-anthraquinone has a flat heterocyclic ring structure, which has been widely used in biological sciences, photochemicals and environmental studies involved with toxic contaminants [20,21]. ARS has been widely used as a sensor for the detection of metal ions and biomolecules because of high fluorescence intensity [22,23].…”

Section: Introductionmentioning

confidence: 99%

Layer-by-Layer Assembly of Electroactive Dye/LDHs Nanoplatelet Matrix Film for Advanced Dual Electro-optical Sensing Applications

2020

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It proved that the most destructive effects of the toxic Al3+ ion on the human nervous system and disease that are involved with this system, such as Alzheimer's. The development of solid-state electrodes is still in its infancy during the sensor-based detection methods for Al3+. Hence, in this study, a novel flexible ITO/PET-based electrochemical solid-state sensor was designed and constructed. Modification of the surface of electrode bedding was done by layer-by-layer (LbL) assembly of Mg–Al LDH. nanoplatelets along with alizarin red S (ARS) in an interconnected matrix film. In the molecular design of sensing base of the electrode, the electroactive organic units (ARS molecules) present in the ITO/PET-layered (ARS/LDHs)n matrix are involved in electrochemical reactions when exposed to the target molecule (Al3+ ion), so the electrochemical changes of the new formed Al-chelated system are detectable. This type of sensor is used for sensitive and selective detection of Al3+. The minimum sheet resistance, morphology and high electrocatalytic activity of the modified matrix film are obtained in the fifth cycle of LbL assembly technique. In this electrochemical sensor, both electrochemical and optical methods were detected with high sensitivity and selectivity of Al3+, so that in a cyclic voltammetry electrochemical method, the lower detection limit of 10.1 nM with a linear range of [0.2–120 μM] was obtained compared to the fluorescence-based optical method.

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“…However, most publications concerned specifically with sulphonated alizarine (ARS) are experimental studies and no calculations were made on its spectral properties [7,[16][17][18][19]. There are articles and reviews dedicated to the testing the TDDFT performance of different xc-functionals which include anthraquinone derivatives [9-12, 20, 21].…”

Section: Introductionmentioning

confidence: 99%

Performance of exchange–correlation functionals on describing ground state geometries and excitations of Alizarin Red S: Effect of complexation and degree of deprotonation

Fehér

Purgel

Joó

2014

Computational and Theoretical Chemistry

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Ground state optimizations and excited state calculations were performed to analyze the possible anionic forms of Alizarin Red S(ulphonated) and its ML 2 type metal complexes formed with palladium(II). Six functionals have been tested (B3LYP, M06-2X, M06, BH&HLYP, PBE0, LC-wPBE, and CAM-B3LYP) with two basis sets (6-311+g(d,p) and TZVP). The relative errors of these functionals in reproducing the experimental UV-Vis absorption wavelengths are reported for the energetically lowest lying isomers. It was found that the degree of deprotonation affects several functionals in a systematic way. M06 and CAM-B3LYP xc-functionals gave the best estimates according to the average relative errors.These two functionals were then used to explore the coordination mode (hydroxy-keto or catechol) of the palladium(II) complexes and the effect of (de)protonation.

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Copper(II)−Alizarin Red S Complex as an Efficient Chemiluminescent Probe for the Detection of Human Serum Proteins after Polyacrylamide Gel Electrophoresis

Cited by 10 publications

References 31 publications

Mass spectrometry of peptides and proteins from human blood

Mass spectrometry of peptides and proteins from human blood

Layer-by-Layer Assembly of Electroactive Dye/LDHs Nanoplatelet Matrix Film for Advanced Dual Electro-optical Sensing Applications

Performance of exchange–correlation functionals on describing ground state geometries and excitations of Alizarin Red S: Effect of complexation and degree of deprotonation

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