Copper exposure effects on yeast mitochondrial proteome

Banci, Lucia; Bertini, Ivano; Ciofi‐Baffoni, Simone; D'Alessandro, Annamaria; Jaiswal, Deepa; Marzano, Valeria; Neri, Sara; Ronci, Maurizio; Urbani, Andrea

doi:10.1016/j.jprot.2011.04.014

Cited by 22 publications

(14 citation statements)

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“…ROS production occurs continuously in varied organelles, and when plants are exposed to high concentrations of this metal, there is an increase in this production (Gratão et al, 2005). Cu is a metal redox, which can catalyze the formation of ROS in Haber-Weiss and Fenton reaction types (Banci et al, 2011). These molecules can induce degradation of biological membranes, chlorophyll breakdown, protein denaturation and DNA mutation (Apel and Hirt, 2004).…”

Section: Introductionmentioning

confidence: 99%

Short-term physiological responses to copper stress in Salvinia auriculata Aubl.

et al. 2014

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Aim: To evaluate, in a short-time exposure, the physiological responses of Salvinia auriculata Aubl. under different concentrations of Cu. Methods: The plants were exposed to treatments with 0.01, 0.1, 1 and 10 mM of Cu in a period of 2 days. Then development variables of S. auriculata (weight, photosynthetic pigments, and soluble carbohydrate), lipid peroxidation (malondialdehyde, aldehydes, and electrolyte leakage) and production of antioxidants (anthocyanins, carotenoids, flavonoids, and proline) were evaluated. Results: It was observed fresh weight reductions in concentrations above 1 mM of Cu. Chlorophyll a decreased with the increase of Cu concentrations unlike chlorophyll b. The ratio chlorophyll a / chlorophyll b was changed due to the degradation of photosynthetic pigments. The reductions of carotenoids were more pronounced than that of total chlorophyll. The values of electrolyte leakage ranged from 14 to 82 % and lipid peroxidation from 7 to 46 nmol.g -1. Flavonoids and soluble carbohydrates showed reductions with the increase of Cu concentration. Anthocyanins, phenolic compounds, and proline when subjected to 0.1 mM of Cu had increased, suggesting adaptability of plant stress caused directly by metal and reactive oxygen species. In higher concentrations, degradation and/or direct modifications of these molecules possibly occurred. Conclusions: The data suggest that S. auriculata is provided with an efficient mechanism against stress caused by Cu in the concentration of 0.1 mM. As for higher concentrations (1 and 10 mM), despite its role as micronutrients, Cu was toxic to the plant due to the redox behavior of this metal, which leads to the exacerbated formation of reactive oxygen species, inducing to severe damage such as biological membrane degradation and protein denaturation.Keywords: lipid peroxidation, antioxidant, proline, metal stress.Resumo: Objetivo: avaliar em curto tempo de exposição às respostas fisiológicas de Salvinia auriculata Aubl. sob diferentes concentrações de Cu. Métodos: as plantas foram expostas a tratamentos com 0,0; 0,01; 0,1; 1 e 10 mM de Cu em um período de 2 dias. Em seguida foram avaliadas variáveis de desenvolvimento de S. auriculata (peso, pigmentos fotossintéticos e carboidratos solúveis), peroxidação lipídica (malonaldeído, aldeídos e extravasamento de eletrólitos) e produção de antioxidantes (antocianinas, carotenóides, flavonóides e prolina). Resultados: Foram verificadas reduções das massas frescas nas concentrações acima de 1 mM de Cu. A clorofila a reduziu com o aumento da concentração de Cu, diferentemente da clorofila b. A razão clorofila a/clorofila b foi alterada, devido à degradação dos pigmentos fotossintéticos. As reduções dos carotenóides foram mais acentuada do que a de clorofila total. Os valores de extravasamento de eletrólitos variaram de 14 a 82 % e peroxidação lipídica de 7 a 46 nmol.g -1. Flavonóides e carboidratos solúveis mostraram reduções com o aumento da concentração de Cu. Antocianinas, compostos fenólicos e prolina quando submetida...

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Section: Introductionmentioning

confidence: 99%

Short-term physiological responses to copper stress in Salvinia auriculata Aubl.

et al. 2014

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“…In addition, the data presented here also highlight the fact that despite the conservation of several important metabolic pathways, there still remain yeast and mammalian cell-specific aspects: while in mammalian cells Cu-induced toxicity is directly related to impact on mitochondrial function 45678, there are contradictory reports regarding Cu-induced toxicity and mitochondrial function in yeast 151617. Indeed, the loss of the cytoplasmic ROS generator Yno1p or cytoplasmic Sod1p increases or decreases yeast tolerance to Cu, respectively, indicating that Cu-induced toxicity in yeast seems associated with detrimental cytoplasmic, but does not exclude, mitochondrial ROS production.…”

Section: Discussionmentioning

confidence: 79%

“…In yeast, there are several contradictory reports on Cu-induced toxicity and mitochondrial dysfunction. Some reports have demonstrated the negative impact of non-lethal Cu doses on the mitochondrial proteome and function in yeast, resulting in decreased ATP production and activation of the oxidative stress response 15, and mitochondrial abnormalities 16. In contrast, Cu-treatment of yeast has also been reported to increase the mitochondrial Cu content without causing respiratory deficits 17.…”

Section: Introductionmentioning

confidence: 99%

Angiotensin II type 1 receptor blockers increase tolerance of cells to copper and cisplatin

Spincemaille

Chandhok

Zibert

et al. 2014

MIC

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The human pathology Wilson disease (WD) is characterized by toxic copper (Cu) accumulation in brain and liver, resulting in, among other indications, mitochondrial dysfunction and apoptosis of hepatocytes. In an effort to identify novel compounds that can alleviate Cu-induced toxicity, we screened the Pharmakon 1600 repositioning library using a Cu-toxicity yeast screen. We identified 2 members of the drug class of Angiotensin II Type 1 receptor blockers (ARBs) that could increase yeast tolerance to Cu, namely Candesartan and Losartan. Subsequently, we show that specific ARBs can increase yeast tolerance to Cu and/or the chemotherapeutic agent cisplatin (Cp). The latter also induces mitochondrial dysfunction and apoptosis in mammalian cells. We further demonstrate that specific ARBs can prevent the prevalence of Cu-induced apoptotic markers in yeast, with Candesartan Cilexetil being the ARB which demonstrated most pronounced reduction of apoptosis-related markers. Next, we tested the sensitivity of a selection of yeast knockout mutants affected in detoxification of reactive oxygen species (ROS) and Cu for Candesartan Cilexetil rescue in presence of Cu. These data indicate that Candesartan Cilexetil increases yeast tolerance to Cu irrespectively of major ROS-detoxifying proteins. Finally, we show that specific ARBs can increase mammalian cell tolerance to Cu, as well as decrease the prevalence of Cu-induced apoptotic markers. All the above point to the potential of ARBs in preventing Cu-induced toxicity in yeast and mammalian cells.

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“…The yeast mitochondria contain a pool of copper (Cobine et al, ; Linder and Hazegh‐Azam, ). This metal is associated with two known mitochondrial cuproenzymes, superoxide dismutase (Sod1) and cytochrome c oxidase and the copper‐binding assembly proteins (Cox11, Cox17, and Sco1; Banci et al, ; Cobine et al, ; De Freitas et al, ; Kako et al, ). Here, the proteome of P. lutzii mitochondria showed a superoxide dismutase (Sod1, PAAG_02725) and two isoforms of cytochrome c oxidase (PAAG_08057 and PAAG_12011), also Soc1, copper‐binding assembly protein (PAAG_06668).…”

Section: Resultsmentioning

confidence: 99%

Analysis ofParacoccidioideslutziimitochondria: a proteomic approach

Casaletti

Lima

Oliveira

et al. 2017

Yeast

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The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signalling centre that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms. The aim of this work was to perform a descriptive proteomic analysis of mitochondria in Paracoccidioides lutzii yeast cells. After mitochondria fractionation, samples enriched in mitochondrial proteins were digested with trypsin and analysed using a NanoUPLC-MS system (Waters Corporation, Manchester, UK). Ours results revealed that the established protocol for purification of mitochondria was very effective for P. lutzii, and 298 proteins were identified as primarily mitochondrial, in our analysis. To our knowledge, this is the first compilation of mitochondrial proteins from P. lutzii, to date. Copyright © 2016 John Wiley & Sons, Ltd.

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Copper exposure effects on yeast mitochondrial proteome

Cited by 22 publications

References 53 publications

Short-term physiological responses to copper stress in Salvinia auriculata Aubl.

Short-term physiological responses to copper stress in Salvinia auriculata Aubl.

Angiotensin II type 1 receptor blockers increase tolerance of cells to copper and cisplatin

Analysis ofParacoccidioideslutziimitochondria: a proteomic approach

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