Copper cofactor delivery in plant cells

Pilon, Marinus; Abdel‐Ghany, Salah E.; Cohu, Christopher M.; Gogolin, Kathryn A.; Hong, Yang

doi:10.1016/j.pbi.2006.03.007

Cited by 212 publications

(121 citation statements)

References 49 publications

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“…Copper, a redox active metal, can catalyze the formation of harmful free radicals such as hydroxyl, peroxyl and alkoxyl radicals, resulting in oxidative stress. Activity of one or more antioxidant enzymes generally increases in plants exposed to stress conditions, and this elevated activity correlates with increased stress tolerance (Pilon et al 2006). Studies have suggested that POD activities in roots were higher than those in leaves and stems.…”

Section: Resultsmentioning

confidence: 99%

“…However, plants usually find an ample supply of copper in soils, and copper at high concentrations can be a stress factor triggering physiological responses (Yruela 2005). At the cellular level, copper is a structural and catalytic component of many proteins and enzymes involved in a variety of metabolic pathways (Pilon et al 2006). It has been previously reported that an excess of Cu can result in production of reactive oxygen species (ROS) and free radicals.…”

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confidence: 99%

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Effects of copper on growth, antioxidant enzymes and phenylalanine ammonia-lyase activities in Jatropha curcas L. seedling

Gao¹,

Yan²,

Cao³

et al. 2008

PLANT SOIL ENVIRON

110

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The effects of different concentrations of copper (0-800 µmol) on growth, protein contents, peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL) in Jatropha curcas L. seedlings were assessed by means of pot experiments. Results suggested that increased copper concentrations lead to decreased shoot elongation and seedling biomass. Protein content in the leaves and roots reached their highest levels at the copper concentrations of 400 µmol, while the highest protein content in the stem was observed at 800 µmol copper. POD activity in leaves and stems was unaffected at low copper concentrations, but showed a considerable variation at high copper concentrations. In roots, the highest POD activity was observed at 200 µmol copper. Under copper stress, SOD activity in leaves increased concomitantly with increasing copper up to 400 µmol, and SOD activity in stems and roots showed a slight increase. Catalase activity significantly elevated in leaves and roots but showed no significant changes in stems of the seedlings exposed to copper. A gradual increase of PAL activity in leaves and roots at the copper concentration of 400 and 200 µmol was observed, while PAL activity remained unchanged in stems.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Effects of copper on growth, antioxidant enzymes and phenylalanine ammonia-lyase activities in Jatropha curcas L. seedling

Gao¹,

Yan²,

Cao³

et al. 2008

PLANT SOIL ENVIRON

110

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“…In plants, copper must be delivered to a number of important enzymes that are active in various subcellular locations (1). One of the most abundant copper proteins in photosynthetic tissues is plastocyanin (PC), 4 which localizes to the thylakoid lumen of chloroplasts and mediates electron transport between the cytochrome b 6 f complex and photosystem I.…”

mentioning

confidence: 99%

“…Therefore the allocation of available copper over these two organelles may also be crucial for maintaining an optimum energy budget in a cell. The copper delivery system to PC and Cu,Zn-SOD in plant cells has been studied in some detail (1). copper enters the cell by means of the CopT family of transporters (8,9).…”

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confidence: 99%

Regulation of Copper Homeostasis by Micro-RNA in Arabidopsis

Yamasaki

Abdel‐Ghany

Cohu

et al. 2007

Journal of Biological Chemistry

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382

376

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Major copper proteins in the cytoplasm of plant cells are plastocyanin, copper/zinc superoxide dismutase, and cytochrome c oxidase. Under copper limited conditions, expression of copper/ zinc superoxide dismutase is down-regulated and the protein is replaced by iron superoxide dismutase in chloroplasts. We present evidence that a micro-RNA, miR398, mediates this regulation in Arabidopsis thaliana, by directing the degradation of copper/zinc superoxide dismutase mRNA when copper is limited. Sequence analysis indicated that the transcripts encoding cytosolic copper/zinc superoxide dismutase and COX5b-1, a subunit of the mitochondrial cytochrome c oxidase, are also targeted by miR398. This regulation via miR398 takes place in response to changes in a low range of copper levels (0.2-0.5 M), indicating that miR398 is involved in a response to copper limitation. On the other hand, another major copper protein, plastocyanin, which is involved in photosynthetic electron flow and is essential in higher plants, was not regulated via miR398. We propose that miR398 is a key factor in copper homeostasis in plants and regulates the stability of mRNAs of major copper proteins under copper-limited conditions.

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“…1 In higher plants, the most abundant copper protein is plastocyanin, which is involved in the photosynthetic electron transport in the thylakoid lumen of chloroplasts. Another major copper protein, Cu/Zn SOD, localizes to the cytoplasm (CSD1), stroma of chloroplasts (CSD2) and also peroxisomes (CSD3), and is involved in the scavenging of reactive oxygen species.…”

mentioning

confidence: 99%