Copper binding by the root cell walls of Italian ryegrass and red clover

Iwasaki, Kō zō; Sakurai, Katsutoshi; Takáhashi, Eiichi

doi:10.1080/00380768.1990.10416911

Cited by 44 publications

(21 citation statements)

References 15 publications

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“…Our results are in good agreement with those of other experiments in which plants were grown in Cu-rich solutions or soils (Ouzounidou et al 1995;Liao et al 2000a;Ali et al 2002). Further investigations into the Cu subcellular localization in roots have revealed that most of Cu was located in root apoplast or cell wall when plants were grown in Cu-rich solution (Thornton 1989;Iwasaki et al 1990;Ouzounidou et al 1995;Jung et al 2003). This is closely related to the cell wall's nature, a weak acid type of natural cation exchanger, by which the cell wall of maize roots has relatively high adsorption capacity for Cu(II) (Haynes 1980;Allan and Jarrell 1989;Iwasaki et al 1990), and and Cys 0 is the neutral free cysteine species.…”

Section: Discussionmentioning

confidence: 95%

Effect of exogenous amino acids on Cu uptake and translocation in maize seedlings

Zhou

Zhang

et al. 2007

Plant Soil

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This study aimed to assess the effects of four contrasting proteinogenic amino acids on copper (Cu) uptake and translocation in maize (Zea mays L.) seedlings grown in a modified Hoagland solution. Glycine, aspartic acid and lysine at three concentrations (10, 25 and 100 lM) did not have any significant effect on Cu uptake and translocation in maize seedlings over a twoday experimental period. However, cysteine (a reductive amino acid) at the three concentrations increased very significantly (P < 0.01) Cu accumulations in the root symplast and the shoots of maize seedlings in comparison to the control. Cu uptake in the whole plant and Cu translocation from root to shoot were also increased in the cysteine treatments. In the 25 lM cysteine treatment, where cysteine was in moderate excess, the Cu uptake in the whole plant and Cu translocation from root to shoot were significantly (P < 0.01) higher than those of the 10 or 100 lM cysteine treatments, where the concentration of cysteine was equivalent to that of Cu(II) or in great excess according to the stoichiometry of the redox reaction of cysteine with Cu(II). It is hypothesized that the cysteineinduced oxidation state alteration from Cu(II) to Cu(I) could be responsible for the increased Cu uptake and Cu translocation, on the ground that Cu(I), as free cuprous ion or cysteine cuprous complex, may be more available to maize roots than Cu(II).

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Section: Discussionmentioning

confidence: 95%

Effect of exogenous amino acids on Cu uptake and translocation in maize seedlings

Zhou

Zhang

et al. 2007

Plant Soil

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“…Another set of frozen root sub-samples was used to measure symplasmic Cu after the extraction of apoplasmic Cu with HCl on thawn roots. The extraction procedure was previously detailed and tested by Chaignon et al (2002), based on the earlier work of Iwasaki et al (1990). Iwasaki et al (1990) had shown in the first place that HCl did not extract root symplasmic Cu, providing the extraction was not too long and conducted under mildly acidic conditions.…”

Section: Plant Measurementsmentioning

confidence: 99%

“…The extraction procedure was previously detailed and tested by Chaignon et al (2002), based on the earlier work of Iwasaki et al (1990). Iwasaki et al (1990) had shown in the first place that HCl did not extract root symplasmic Cu, providing the extraction was not too long and conducted under mildly acidic conditions. Chaignon et al (2002) later checked that freezing and thawing of roots before the extraction and HCl concentrations (10 −3 and 10 −2 M for short periods of time) used during the extraction were not affecting the integrity of root plasma membrane, in order to avoid any overestimation of Cu binding in root apoplasm.…”

Section: Plant Measurementsmentioning

confidence: 99%

Copper uptake kinetics in hydroponically-grown durum wheat (Triticum turgidum durum L.) as compared with soil’s ability to supply copper

et al. 2009

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This study investigated (a) net Cu uptake kinetics in durum wheat (Triticum turgidum durum L.) exposed to free Cu 2+ activities in solution ranging from 0.4 to 2,420 nM and (b) the relative importance of plant uptake and soil's ability to supply Cu 2+ to the roots. Plant Cu flux showed a hyperbolic shape, enabling to estimate the Michaelis-Menten kinetic parameters (F max and K M ) for durum wheat. Plant Cu flux was then compared with soil Cu flux as assessed by the Diffusive Gradient in Thin film technique on seven soil samples. This comparison suggested that the rate-limiting process of Cu bioavailability to durum wheat would be plant uptake kinetics in most contaminated soils with the exception of moderately contaminated, calcareous soils. However, theoretical considerations targeted soil's ability to supply Cu as the rate-limiting process in most soils for Cu (hyper-) accumulator plants with requirement larger than that of common crop species.

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“…The lignified root tips lose their capacity for nutrient uptake and root growth stops (Punz and Sieghardt 1993;Kahle 1993;Schützendübel and Polle 2002). About 60 % of Cu in the roots of Lolium multiflorum (Italian ryegrass) and T. pratense are reported to be bound by the cell wall and plasma membrane (Iwasaki et al 1990). Minuartia verna ssp.…”

Section: Cell Wallmentioning

confidence: 99%