Copper addition helps alleviate iron stress in a coastal diatom: Response of Chaetoceros gracilis from the Bay of Bengal to experimental Cu and Fe addition

Biswas, Haimanti; Bandyopadhyay, Debkumar; Waite, Anya M.

doi:10.1016/j.marchem.2013.10.006

Cited by 18 publications

(11 citation statements)

References 72 publications

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“…Some phytoplankton have higher Cu demands during Fe limitation (Peers et al, 2005;Wells et al, 2005;Maldonado et al, 2006;Annett et al, 2008;Guo et al, 2012;Biswas et al, 2013). Oceanic phytoplankton strains have higher basal metabolic Cu requirements compared to coastal strains, and may reflect an increased reliance on Cu in waters with chronically low Fe (Peers and Price, 2006;Annett et al, 2008).…”

Section: Environmental Controls Of Biogenic Cu Along Line Pmentioning

confidence: 99%

“…Indeed, some Fe-limited phytoplankton have higher metabolic dependencies on Cu (Peers et al, 2005;Annett et al, 2008;Semeniuk et al, 2009;Guo et al, 2012;Biswas et al, 2013). This may be due to upregulation of the Cu-containing photosynthetic electron shuttle plastocyanin and the multiple-Cu containing oxidase component of a high affinity Fe transport system in diatoms (Maldonado et al, 2006;Peers and Price, 2006;Kustka et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

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Using 67Cu to Study the Biogeochemical Cycling of Copper in the Northeast Subarctic Pacific Ocean

et al. 2016

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Microbial copper (Cu) nutrition and dissolved Cu speciation were surveyed along Line P, a coastal to open ocean transect that extends from the coast of British Columbia, Canada, to the high-nutrient-low-chlorophyll (HNLC) zone of the northeast subarctic Pacific Ocean. Steady-state size fractionated Cu uptake rates and Cu:C assimilation ratios were determined at in situ Cu concentrations and speciation using a 67 Cu tracer method. The cellular Cu:C ratios that we measured (∼30 μmol Cu mol C −1 ) are similar to recent estimates using synchrotron x-ray fluorescence (SXRF), suggesting that the 67 Cu method can determine in situ metabolic Cu demands. We examined how environmental changes along the Line P transect influenced Cu metabolism in the sub-microplankton community. Cellular Cu:C assimilation ratios and uptake rates were compared with net primary productivity, bacterial abundance and productivity, total dissolved Cu, Cu speciation, and a suite of other chemical and biological parameters. Total dissolved Cu concentrations ([Cu] d ) were within a narrow range (1.5-2.8 nM), and Cu was bound to a ∼5-fold excess of strong ligands with conditional stability constants (K cond 2 ) of ∼10 14 .CuL,Cu + Free Cu 2+ concentrations were low (pCu 14.4-15.1), and total and size fractionated net primary productivity (NPP ; g C L −1 d −1 V μ ) were negatively correlated with inorganic Cu concentrations ([Cu ]). We suggest this is due to greater Cu drawdown by faster growing phytoplankton populations. Using the relationship between [Cu ] drawdown and NPP V , we calculated a regional photosynthetic Cu:C drawdown export ratio between 1.5 and 15 μmol Cu mol C −1 , and a mixed layer residence time (2.5-8 years) that is similar to other independent estimates (2-12 years). Total particulate Cu uptake rates were between 22 and 125 times faster than estimates of Cu export; this is possibly mediated by rapid cellular Cu uptake and efflux by phytoplankton and bacteria or the effects of grazers and bacterial remineralization on dissolved Cu. These results provide a more detailed understanding of the interactions between Cu speciation and microorganisms in seawater, and suggest that marine phytoplankton modify Cu speciation in the open ocean.

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Section: Environmental Controls Of Biogenic Cu Along Line Pmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Using 67Cu to Study the Biogeochemical Cycling of Copper in the Northeast Subarctic Pacific Ocean

et al. 2016

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“…Finally, we observed with increased phytoplankton growth under Cu stress. The available literature suggests that modestly elevated concentrations of Cu can significantly affect the growth and primary production of coastal diatoms (Biswas, Bandyopadhyay, & Waite, ; Peers, Quesnel, & Price, ). Semeniuk et al.…”

Section: Resultsmentioning

confidence: 99%

“…The Finally, we observed with increased phytoplankton growth under Cu stress. The available literature suggests that modestly elevated concentrations of Cu can significantly affect the growth and primary production of coastal diatoms (Biswas, Bandyopadhyay, & Waite, 2013;Peers, Quesnel, & Price, 2005). Semeniuk et al (2009) reported that high levels of dissolved Cu are responsible for the high abundance of phytoplankton in the subarctic northeast Pacific Ocean.…”

Section: Possible Ecological Consequences Of Cu Stress On the Oceanmentioning

confidence: 99%

Increasing copper alters cellular elemental composition (Mo and P) of marine diatom

Wang

Xia

Kumar

et al. 2017

Ecology and Evolution

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The elemental composition (surface adsorbed and internalized fraction of Cu, Mo and P) in marine phytoplankton was first examined in cultures of the diatom Phaeodactylum tricornutum which were exposed to various levels of Cu concentrations ranging from 0.25 to 16 μmol/L with equivalent free [Cu2+] concentrations of 0.4–26 nmol/L. We observed an acceleration of algal growth rates (20–40%) with increasing ambient Cu levels, as well as slightly increased levels of internalized Cu in cells (2–13 × 10−18 mol/cell) although cellular Cu mostly accumulated onto the cell surface (>50% of the total: intracellular + surface adsorbed). In particular, we documented for the first time that the elemental composition (Mo and P) in algal cells varies dynamically in response to increased Cu levels: (1) Cellular P, predominantly in the intracellular compartment (>95%), shows with a net consumption as indicated by a gradual decrease with increasing [Cu2+] (120→50 × 10−15 mol P/cell) probably due to the fact that P, a backbone bioelement, is largely required in forming biological compartments such as cell membranes; and (2) cellular Mo, predominantly encountered in the intracellular compartment, showed up to tenfold increase in concentration in the cultures exposed to Cu, with a peak accumulation of 1.1 × 10−18 mol Mo/cell occurring in the culture exposed to [Cu2+] at 3.7 nmol/L. Such a net cellular Mo accumulation suggests that Mo might be specifically required in biological processes, probably playing a counteracting role against Cu.

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“…Increases in dissolved zinc and manganese reduced sensitivity to added Cu in situ (Braek et al, 1976;Jensen et al, 1976;Kazumi et al, 1987;Coale, 1991) and in vivo (Sunda andHuntsman, 1983, 1998), and both Stauber and Florence (1985) and Maldonado et al (2002) documented reductions in Cu sensitivity following Fe-enrichment. Alternatively, Cu toxicity was exacerbated by increases in cadmium, zinc, and lead (Bartlett and Rabe, 1973;Saifullah, 1984, 1986), and Cu availability alters Fe-quotas and uptake in various isolates and even heterogeneous field populations (Peers et al, 2005;Wells et al, 2005;Maldonado et al, 2006;Guo et al, 2012;Biswas et al, 2013;Lelong et al, 2013;Semeniuk et al, 2016). The relatively short timescale of Cu toxicity experiments allows for many experimental treatments.…”

Section: Macronutrient and Micronutrient Availability Should Be Consimentioning

confidence: 99%

The Toxicity of Copper to Crocosphaera watsonii and Other Marine Phytoplankton: A Systematic Review

2019

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The cupric ion (Cu 2+) plays a dual role as both nutrient and toxicant to freshwater and marine phytoplankton, functioning in multiple photochemical processes, as well as reactive oxygen species (ROS) production. This duality has been investigated through a variety of methods to determine the consequences of natural and anthropogenic copper introduction to algal ecosystem composition. Studies conducted over the past few decades have described the growth responses of many unique organisms to copper availability. Such observations are critical for describing the global distributions of major phytoplankton species in terms of global trace metal abundance; however, highly variable experimental practices impede direct inter-study comparison. The aim of this systematic review is to summarize the available data regarding the effects of copper concentration on diverse marine phytoplankton growth rates. Through extensive literary comparison, 143 studies were systematically reviewed, and data on copper concentrations and growth rates reported were extracted. From the data available, we conclude that trends in phytoplankton sensitivity to copper are mainly driven by a single study. We discuss the obstacles to inter-study comparison and detail both the concurring and conflicting results to date, with an emphasis on taxonomic trends and methodologies employed. Finally, we present the first copper sensitivity measurements for marine unicellular diazotrophs using three representative strains of the unicellular nitrogen-fixing cyanobacterium, Crocosphaera watsonii.

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Copper addition helps alleviate iron stress in a coastal diatom: Response of Chaetoceros gracilis from the Bay of Bengal to experimental Cu and Fe addition

Cited by 18 publications

References 72 publications

Using 67Cu to Study the Biogeochemical Cycling of Copper in the Northeast Subarctic Pacific Ocean

Using 67Cu to Study the Biogeochemical Cycling of Copper in the Northeast Subarctic Pacific Ocean

Increasing copper alters cellular elemental composition (Mo and P) of marine diatom

The Toxicity of Copper to Crocosphaera watsonii and Other Marine Phytoplankton: A Systematic Review

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