Copolymers of Ethylene Imine and <i>N</i>-(2-Hydroxyethyl)-ethylene Imine as Tools To Study Effects of Polymer Structure on Physicochemical and Biological Properties of DNA Complexes

Fischer, Dagmar; Harpe, Anke von; Kunath, Klaus; Petersen, Holger; Li, Youxin; Kissel, Thomas

doi:10.1021/bc025550w

Cited by 99 publications

(70 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] There has been considerable interest in characterizing the nature of the fundamental interactions between cationic agents and DNA, and several general approaches have been designed to unravel such interactions. [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] One of the important driving forces in all of these systems is the long-range electrostatic interaction between molecules of opposite charge. Among all of the cationic agents, single tail lipid molecules, i.e., surfactants, have been studied extensively.…”

Section: Introductionmentioning

confidence: 99%

Effect of Headgroup on DNA−Cationic Surfactant Interactions

Dasgupta¹,

Das²,

Dias³

et al. 2007

J. Phys. Chem. B

View full text Add to dashboard Cite

The interaction behavior of DNA with different types of hydroxylated cationic surfactants has been studied. Attention was directed to how the introduction of hydroxyl substituents at the headgroup of the cationic surfactants affects the compaction of DNA. The DNA-cationic surfactant interaction was investigated at different charge ratios by several methods like UV melting, ethidium bromide exclusion, and gel electrophoresis. Studies show that there is a discrete transition in the DNA chain from extended coils (free chain) to a compact form and that this transition does not depend substantially on the architecture of the headgroup. However, the accessibility of DNA to ethidium bromide is preserved to a significantly larger extent for the more hydrophilic surfactants. This was discussed in terms of surfactant packing. Observations are interpreted to reflect that the surfactants with more substituents have a larger headgroup and therefore form smaller micellar aggregates; these higher curvature aggregates lead to a less efficient, "patch-like" coverage of DNA. The more hydrophilic surfactants also presented a significantly lower cytotoxicity, which is important for biotechnological applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of Headgroup on DNA−Cationic Surfactant Interactions

Dasgupta¹,

Das²,

Dias³

et al. 2007

J. Phys. Chem. B

View full text Add to dashboard Cite

show abstract

“…Hyperbranched cationic glycogen derivatives as efficient gene vectors the DMAPA-Glyp/pDNA and the AEPZ-Glyp/pDNA complexes, respectively (lanes [3][4][5][6][7][8][9][10][11][12][13][14]. This demonstrated that the glycogen derivatives exhibited distinct protective effects against DNase I.…”

mentioning

confidence: 81%

“…21 Therefore, the result may indicate that pDNA-binding and -condensation abilities are enhanced with the increase in the fraction of branching units of the hyperbranched polysaccharide derivatives, in agreement with the literature. [4][5][6][7] This is closely related to their genedelivery efficacy, which needs to be investigated in the future.…”

Section: Buffer Capacitymentioning

confidence: 99%

“…Recent work has established that the architecture of cationic polymers affects the stability, delivery, and transfection efficiency of their complexes with deoxyribonucleic acid (DNA). [3][4][5][6][7][8] In contrast to linear cationic polymers, hyperbranched cationic polymers exhibit higher levels of gene expression, probably because of the following two factors: 1) they present compact and globular structures in combination with a significant number and variety of different amine groups, which are advantageous for the binding and condensation of DNA into compact particles, resulting in improved protection and endocytosis of DNA; [4][5][6][7] and 2) they exhibit better proton-buffer capacity, which is beneficial for the escape of DNA from endosomes. 4,8 Synthetic hyperbranched cationic polymers, however, such as branched polyethyleneimine (bPEI) and poly(amidoamine) (PAMAM) dendrimers, are still associated with cytotoxicity, biocompatibility, and biodegradability issues, which need to be overcome to allow for in vivo application.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

Liu¹,

Ren²,

Liu³

et al. 2014

IJN

View full text Add to dashboard Cite

Background The purpose of this study was to synthesize and evaluate hyperbranched cationic glycogen derivatives as an efficient nonviral gene-delivery vector. Methods A series of hyperbranched cationic glycogen derivatives conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Glyp) and 1-(2-aminoethyl) piperazine (AEPZ-Glyp) residues were synthesized and characterized by Fourier-transform infrared and hydrogen-1 nuclear magnetic resonance spectroscopy. Their buffer capacity was assessed by acid–base titration in aqueous NaCl solution. Plasmid deoxyribonucleic acid (pDNA) condensation ability and protection against DNase I degradation of the glycogen derivatives were assessed using agarose gel electrophoresis. The zeta potentials and particle sizes of the glycogen derivative/pDNA complexes were measured, and the images of the complexes were observed using atomic force microscopy. Blood compatibility and cytotoxicity were evaluated by hemolysis assay and MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively. pDNA transfection efficiency mediated by the cationic glycogen derivatives was evaluated by flow cytometry and fluorescence microscopy in the 293T (human embryonic kidney) and the CNE2 (human nasopharyngeal carcinoma) cell lines. In vivo delivery of pDNA in model animals (Sprague Dawley rats) was evaluated to identify the safety and transfection efficiency. Results The hyperbranched cationic glycogen derivatives conjugated with DMAPA and AEPZ residues were synthesized. They exhibited better blood compatibility and lower cytotoxicity when compared to branched polyethyleneimine (bPEI). They were able to bind and condense pDNA to form the complexes of 100–250 nm in size. The transfection efficiency of the DMAPA-Glyp/pDNA complexes was higher than those of the AEPZ-Glyp/pDNA complexes in both the 293T and CNE2 cells, and almost equal to those of bPEI. Furthermore, pDNA could be more safely delivered to the blood vessels in brain tissue of Sprague Dawley rats by the DMAPA-Glyp derivatives, and then expressed as green fluorescence protein, compared with the control group. Conclusion The hyperbranched cationic glycogen derivatives, especially the DMAPA-Glyp derivatives, showed high gene-transfection efficiency, good blood compatibility, and low cyto toxicity when transfected in vitro and in vivo, which are novel potential nonviral gene vectors.

show abstract

“…Therefore, polyethylene glycol (PEG) with neutral hydrophilic polymers is attached to polyethyleneimine (PEG-g-PEI) to reduce cytotoxicity while maintaining considerable siRNA transfer efficiency. [7][8][9][10][11] In our previous study, PEG-g-PEI was shown to be a desirable nanoparticle carrier with relatively high siRNA delivery efficiency and low cytotoxicity. 12 Meanwhile, magnetic resonance imaging (MRI) techniques have great advantages in monitoring targeting events and therapeutic outcomes noninvasively.…”

Section: Introductionmentioning

confidence: 99%

Development of an MRI-visible nonviral vector for siRNA delivery targeting gastric cancer

Chen¹,

Wang²,

Lian³

et al. 2012

IJN

View full text Add to dashboard Cite

An antibody-directed nonviral vector, polyethylene glycol-grafted polyethylenimine functionalized with superparamagnetic iron oxide nanoparticles and a gastric cancer-associated CD44v6 single-chain variable fragment (scFv CD44v6 ,-PEG-g-PEI-SPION), was constructed as a gastric cancer-targeting and magnetic resonance imaging (MRI)-visible nanocarrier for small interfering RNA (siRNA) delivery. Biophysical characterization of PEG-g-PEI-SPION and scFv CD44v6 -PEG-g-PEI-SPION was carried out, including siRNA condensation capacity, cell viability, and transfection efficiency. Both the targeting and nontargeting nanocarriers were effective for transferring siRNA in vitro. The cellular uptake and distribution of nanoparticles complexed with siRNA was analyzed by fluorescence imaging and immunofluorescent staining. Moreover, the gastric cancer-targeting effect was verified in vivo by MRI and histology analysis. These results indicate that scFv CD44v6 -PEG-g-PEI-SPION is a promising nonviral vector for gastric cancer gene therapy and diagnosis.

show abstract

Copolymers of Ethylene Imine and N-(2-Hydroxyethyl)-ethylene Imine as Tools To Study Effects of Polymer Structure on Physicochemical and Biological Properties of DNA Complexes

Cited by 99 publications

References 29 publications

Effect of Headgroup on DNA−Cationic Surfactant Interactions

Effect of Headgroup on DNA−Cationic Surfactant Interactions

An efficient nonviral gene-delivery vector based on hyperbranched cationic glycogen derivatives

Development of an MRI-visible nonviral vector for siRNA delivery targeting gastric cancer

Contact Info

Product

Resources

About