COP1 SUPPRESSOR 6 represses the PIF4 and PIF5 action to promote light‐inhibited hypocotyl growth

Lan, Hongxia; Heng, Yueqin; Li, Jian; Zhang, Mengdi; Bian, Yeting; Chu, Li; Jiang, Yan; Wang, Xuncheng; Xu, Dongqing; Deng, Xing Wang

doi:10.1111/jipb.13350

Cited by 4 publications

(4 citation statements)

References 56 publications

(106 reference statements)

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“…To better understand the basis of COP1 function, we performed a genetic screen to identify the suppressors of cop1-6 in the dark. In addition to the published recessive cop1-6 suppressor mutants [42][43][44][45][46][47] , we identified and isolated 13 additional extragenic dominant suppressors of cop1-6 (Fig. 1a).…”

Section: Resultsmentioning

confidence: 99%

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Light regulates nuclear detainment of intron-retained transcripts through COP1-spliceosome to modulate photomorphogenesis

Zhou,

Zeng,

Yan

et al. 2024

Nat Commun

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Intron retention (IR) is the most common alternative splicing event in Arabidopsis. An increasing number of studies have demonstrated the major role of IR in gene expression regulation. The impacts of IR on plant growth and development and response to environments remain underexplored. Here, we found that IR functions directly in gene expression regulation on a genome-wide scale through the detainment of intron-retained transcripts (IRTs) in the nucleus. Nuclear-retained IRTs can be kept away from translation through this mechanism. COP1-dependent light modulation of the IRTs of light signaling genes, such as PIF4, RVE1, and ABA3, contribute to seedling morphological development in response to changing light conditions. Furthermore, light-induced IR changes are under the control of the spliceosome, and in part through COP1-dependent ubiquitination and degradation of DCS1, a plant-specific spliceosomal component. Our data suggest that light regulates the activity of the spliceosome and the consequent IRT nucleus detainment to modulate photomorphogenesis through COP1.

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Section: Resultsmentioning

confidence: 99%

“…Previous studies have reported that the cop1-6 recessive suppressers csu1 to csu6 have distinct functions [42][43][44][45][46][47] . Given our data suggesting that all six of the DCS proteins are components of the spliceosome, we considered COP1's potential function in mRNA splicing.…”

Section: Light and Cop1 Control Genome-wide Alternative Splicing In A...mentioning

confidence: 99%

Light regulates nuclear detainment of intron-retained transcripts through COP1-spliceosome to modulate photomorphogenesis

Zhou,

Zeng,

Yan

et al. 2024

Nat Commun

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“…The PIF4-ABI4 complex synergistically promotes primary seed dormancy Transcription factor PIF4 was initially characterized as a key component of phytochrome-and auxin-signaling pathways (Lau and Deng, 2010;Zhao and Bao, 2021), which served in several biological processes, including young seedling hypocotyl elongation (Gao et al, 2022;Lan et al, 2022), plant thermomorphogenesis, heat response and leaf senescence (Li et al, 2021;Lee et al, 2021b), as well as in response to several phytohormones and diverse environmental cues (Choi and Oh, 2016;Huang et al, 2019;Yan et al, 2020b;Qiu et al, 2021;Xu and Zhu, 2021;.…”

Section: Discussionmentioning

confidence: 99%

PIF4 interacts with ABI4 to serve as a transcriptional activator complex to promote seed dormancy by enhancing ABA biosynthesis and signaling

Luo,

Dai,

Xian

et al. 2024

JIPB

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Transcriptional regulation plays a key role in the control of seed dormancy, and many transcription factors (TFs) have been documented. However, the mechanisms underlying the interactions between different TFs within a transcriptional complex regulating seed dormancy remain largely unknown. Here, we showed that TF PHYTOCHROME‐INTERACTING FACTOR4 (PIF4) physically interacted with the abscisic acid (ABA) signaling responsive TF ABSCISIC ACID INSENSITIVE4 (ABI4) to act as a transcriptional complex to promote ABA biosynthesis and signaling, finally deepening primary seed dormancy. Both pif4 and abi4 single mutants exhibited a decreased primary seed dormancy phenotype, with a synergistic effect in the pif4/abi4 double mutant. PIF4 binds to ABI4 to form a heterodimer, and ABI4 stabilizes PIF4 at the protein level, whereas PIF4 does not affect the protein stabilization of ABI4. Subsequently, both TFs independently and synergistically promoted the expression of ABI4 and NCED6, a key gene for ABA anabolism. The genetic evidence is also consistent with the phenotypic, physiological and biochemical analysis results. Altogether, this study revealed a transcriptional regulatory cascade in which the PIF4–ABI4 transcriptional activator complex synergistically enhanced seed dormancy by facilitating ABA biosynthesis and signaling.

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“…The regulation and interaction of one TF by another is an efficient and effective mechanism to respond to environmental stimuli. For example, Arabidopsis CONSTITUTIVELY PHOTOMORPHOGENIC 1 SUPPRESSOR 6 (CSU6) represses hypocotyl growth via transcriptional and biochemical regulation of PHYTOCHROME‐INTERACTING FACTOR 4 (PIF4) and PIF5 (Lan et al, 2022). By contrast, one recent study showed that a bHLH TF, bHLH38, dually regulates OsDREB2A at the transcriptional level and via direct protein–protein interaction to promote stress tolerance (Du et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

OsNAC5 orchestrates OsABI5 to fine‐tune cold tolerance in rice

Li,

Song,

Wang

et al. 2024

JIPB

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Due to its tropical origins, rice (Oryza sativa) is susceptible to cold stress, which poses severe threats to production. OsNAC5, a NAC‐type transcription factor, participates in the cold stress response of rice, but the detailed mechanisms remain poorly understood. Here, we demonstrate that OsNAC5 positively regulates cold tolerance at germination and in seedlings by directly activating the expression of ABSCISIC ACID‐INSENSITIVE 5 (OsABI5). Haplotype analysis indicated that single nucleotide polymorphisms in a NAC‐binding site in the OsABI5 promoter are strongly associated with cold tolerance. OsNAC5 also enhanced OsABI5 stability, thus regulating the expression of cold‐responsive (COR) genes, enabling fine‐tuned control of OsABI5 action for rapid, precise plant responses to cold stress. DNA affinity purification sequencing coupled with transcriptome deep sequencing identified several OsABI5 target genes involved in COR expression, including DEHYDRATION‐RESPONSIVE ELEMENT BINDING FACTOR 1A (OsDREB1A), OsMYB20, and PEROXIDASE 70 (OsPRX70). In vivo and in vitro analyses suggested that OsABI5 positively regulates COR gene transcription, with marked COR upregulation in OsNAC5‐overexpressing lines and downregulation in osnac5 and/or osabi5 knockout mutants. This study extends our understanding of cold tolerance regulation via OsNAC5 through the OsABI5‐CORs transcription module, which may be used to ameliorate cold tolerance in rice via advanced breeding.This article is protected by copyright. All rights reserved.

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COP1 SUPPRESSOR 6 represses the PIF4 and PIF5 action to promote light‐inhibited hypocotyl growth

Cited by 4 publications

References 56 publications

Light regulates nuclear detainment of intron-retained transcripts through COP1-spliceosome to modulate photomorphogenesis

Light regulates nuclear detainment of intron-retained transcripts through COP1-spliceosome to modulate photomorphogenesis

PIF4 interacts with ABI4 to serve as a transcriptional activator complex to promote seed dormancy by enhancing ABA biosynthesis and signaling

OsNAC5 orchestrates OsABI5 to fine‐tune cold tolerance in rice

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