Coordination of cell cycle and cytokinesis in Trypanosoma brucei

McKean, Paul G.

doi:10.1016/j.mib.2003.10.010

Cited by 99 publications

(106 citation statements)

References 42 publications

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“…Furthermore, a strong TbDLP signal is detected next to the mitochondrial DNA. In T. brucei the highly concatenated mitochondrial genome, termed the kDNA, is not dispersed throughout the matrix but localized at a precise position opposite to the basal body of the flagellum and thus can easily be visualized by DAPI staining (McKean, 2003;Ogbadoyi et al, 2003). The localization of the kDNA-associated HA-tagged TbDLP was further analyzed by double staining with either the FP marker tomato lectin (Fig.…”

Section: Tbdlp Shows a Dual Localizationmentioning

confidence: 99%

“…Unlike the very dynamic mitochondria of mammalian cells (Scott et al, 2003), the T. brucei mitochondrion does not undergo any fission events throughout the cell cycle. Only before or during cytokinesis does the mitochondrion divide in two in order to allow its transmission to the daughter cells (McKean, 2003). Thus, in T. brucei mitochondrial fission must be coordinated with the cell cycle.…”

Section: Introductionmentioning

confidence: 99%

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Ablation of the single dynamin of T. brucei blocks mitochondrial fission and endocytosis and leads to a precise cytokinesis arrest

Chanez

Hehl

Engstler

et al. 2006

Journal of Cell Science

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Section: Tbdlp Shows a Dual Localizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ablation of the single dynamin of T. brucei blocks mitochondrial fission and endocytosis and leads to a precise cytokinesis arrest

Chanez

Hehl

Engstler

et al. 2006

Journal of Cell Science

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“…Whereas most organisms have a single type of mitochondrial chromosome, the mitochondrial DNA in T. brucei consists of two genetic elements, the maxicircles and the minicircles, which are topologically intertwined. Replication of the genome is restricted to a 'mitochondrial S-phase' that is strictly coordinated with the S-phase of the cell cycle [6]. Segregation of the replicated mitochondrial DNA is directly linked to the segregation of the flagellar basal body, and mitochondrial division seems to be a checkpoint for cytokinesis [7].…”

Section: Introductionmentioning

confidence: 99%

The direct route: a simplified pathway for protein import into the mitochondrion of trypanosomes

Schneider

Bursać

Lithgow

2008

Trends in Cell Biology

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“…Due to the single-unit nature of the mitochondrion, its duplication must be coordinated with the duplication of the nucleus (4). The mitochondrial genome of T. brucei, termed kinetoplast DNA (kDNA), is essential for growth of both the procyclic insect stage and the bloodstream form of the parasite (5).…”

mentioning

confidence: 99%

Trypanosomal TAC40 constitutes a novel subclass of mitochondrial β-barrel proteins specialized in mitochondrial genome inheritance

Schnarwiler

Niemann

Doiron

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Mitochondria cannot form de novo but require mechanisms allowing their inheritance to daughter cells. In contrast to most other eukaryotes Trypanosoma brucei has a single mitochondrion whose single-unit genome is physically connected to the flagellum. Here we identify a β-barrel mitochondrial outer membrane protein, termed tripartite attachment complex 40 (TAC40), that localizes to this connection. TAC40 is essential for mitochondrial DNA inheritance and belongs to the mitochondrial porin protein family. However, it is not specifically related to any of the three subclasses of mitochondrial porins represented by the metabolite transporter voltage-dependent anion channel (VDAC), the protein translocator of the outer membrane 40 (TOM40), or the fungi-specific MDM10, a component of the endoplasmic reticulum-mitochondria encounter structure (ERMES). MDM10 and TAC40 mediate cellular architecture and participate in transmembrane complexes that are essential for mitochondrial DNA inheritance. In yeast MDM10, in the context of the ERMES, is postulated to connect the mitochondrial genomes to actin filaments, whereas in trypanosomes TAC40 mediates the linkage of the mitochondrial DNA to the basal body of the flagellum. However, TAC40 does not colocalize with trypanosomal orthologs of ERMES components and, unlike MDM10, it regulates neither mitochondrial morphology nor the assembly of the protein translocase. TAC40 therefore defines a novel subclass of mitochondrial porins that is distinct from VDAC, TOM40, and MDM10. However, whereas the architecture of the TAC40-containing complex in trypanosomes and the MDM10-containing ERMES in yeast is very different, both are organized around a β-barrel protein of the mitochondrial porin family that mediates a DNA-cytoskeleton linkage that is essential for mitochondrial DNA inheritance.organelle | parasitic protozoa

show abstract

Coordination of cell cycle and cytokinesis in Trypanosoma brucei

Cited by 99 publications

References 42 publications

Ablation of the single dynamin of T. brucei blocks mitochondrial fission and endocytosis and leads to a precise cytokinesis arrest

Ablation of the single dynamin of T. brucei blocks mitochondrial fission and endocytosis and leads to a precise cytokinesis arrest

The direct route: a simplified pathway for protein import into the mitochondrion of trypanosomes

Trypanosomal TAC40 constitutes a novel subclass of mitochondrial β-barrel proteins specialized in mitochondrial genome inheritance

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