Coordination-Driven Self-Assembly of Triazole-Based Apoptosis-Inducible Metallomacrocycles

Abstract

Ru(II)-metallomacrocycles containing 4-pyridyl-1,2,3-triazole moiety were realized by coordination-driven self-assembly. All new compounds were characterized by electrospray ionisation mass spectrometry, elemental analysis, and 1 H and 13 C NMR spectroscopic techniques. The molecular structure of metallomacrocycle 8 was determined by single-crystal X-ray crystallography. The anticancer activities of metallomacrocycles 5 – 8 were evaluated by cytotoxicity, cell cycle analysis, and related protein expression. Metallomacrocycle 7 showed the highest cytotoxicity in HepG2 human hepatocellular carcinoma cells. In addition, apoptotic HepG2 cells were analyzed when metallomacrocycle 7 was treated. Our results suggest that metallomacrocycle 7 induces liver cancer cell death by increasing apoptosis and cell cycle arrest and that it has potential use as an agent for the treatment of human hepatocellular carcinoma.

The Hexacoordinate Si Complex SiCl4(4-Azidopyridine)2—Crystallographic Characterization of Two Conformers and Probing the Influence of SiCl4-Complexation on a Click Reaction with Phenylacetylene

“…Bridging thiocyanate (SCN − ) and terminal 4-azidopyridine ligands were reported for Ni complexes [80]. Products of (3+2)-cycloadditions (some triazoles obtained from azidopyridines), however, may then serve as network-forming ligands as well, like in some Cd complexes [81], and bridges in macrocycles, like in some Ru complexes [82,83]. 1 The data set of compound 1,4-3 was collected from a twinned crystal (1 0 0, 0.137 −1 0, 0.853 0 and the structure was refined as a two-domain twin using a HKLF5 format data set, which contai isolated reflections of the predominant domain and overlapping reflections.…”

Step‐By‐Step Tips to Grow X‐Ray Quality Single Crystals of Metal Macrocyclic Complexes

“…In particular, the "vapor deposition method" (VDM) and "layering method" (LM) are the most promising methods for the growth of single crystals from metal macrocyclic complexes, as crystals can be grown even from only a few milligrams of the macrocycles. [ 27–36 ]…”

“…In particular, the "vapor deposition method" (VDM) and "layering method" (LM) are the most promising methods for the growth of single crystals from metal macrocyclic complexes, as crystals can be grown even from only a few milligrams of the macrocycles. [27][28][29][30][31][32][33][34][35][36] Several parameters facilitate single crystal growth including the choice of the solvents and counter-solvents and the surrounding environment. Because the solubility and physical behavior of the sample vary with the solvent, the choice of the crystallization solvent is important.…”