Coordinated transcriptomics and peptidomics of central nervous system identify neuropeptides and their G protein-coupled receptors in the oriental fruit moth Grapholita molesta

Cheng, Jie; Yang, Xuelin; Tian, Zhiqiang; Shen, Zhizhang; Wang, Xueli; Zhu, Lin; Liu, Xiaoming; Li, Zhen; Liu, Xiaoxia

doi:10.1016/j.cbd.2021.100882

Cited by 3 publications

(8 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The HcLK genes were expressed in various tissues of H. cunea , especially highly expressed in the midgut and hindgut, as has been demonstrated in several insect species ( Terhzaz et al, 1999 ; Kwon et al, 2016 ). In Grapholita molesta , LK was predominately expressed in the gut and FB ( Cheng et al, 2021 ), whereas in Chilo suppressalis , LK were predominately expressed in the CNS and gut ( Xu et al, 2016 ). Seven transmembrane domains involved in GPCR ligand binding and receptor activation are functionally conserved in HcLKR , which contains amino acid motifs typical of the GPCR family ( Marco et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Functional Identification and Characterization of Leucokinin and Its Receptor in the Fall Webworm, Hyphantria cunea

Sun

Gao

et al. 2021

Front. Physiol.

View full text Add to dashboard Cite

Neuropeptides function as central neuromodulators and circulating hormones that modulate insect behavior and physiology. Leucokinin (LK) is an intercellular signaling molecule that mediates many physiological and behavioral processes. However, the functions of LK associated with environmental stress and feeding behavior in the fall webworm, Hyphantria cunea, is little known. Our primary objective is to understand the function of LK and LK receptor (LKR) neuroendocrine system in H. cunea. In the present study, the results showed that LK/LKR are expressed at different developmental stages and in various tissues of H. cunea. A candidate receptor–ligand pairing for LK was identified in the larval transcriptome of H. cunea. In a heterologous expression system, the calcium assay was used to demonstrate that LKR is activated by HcLKs in a dose-dependent manner, with 50% effective concentration (EC50) values of 8.44–90.44nM. Knockdown of HcLK and HcLKR by microinjecting target-specific dsRNA leads to several effects in H. cunea, including feeding promotion, increase in resistance to desiccation and starvation stress, and regulation of water homeostasis. The transcript levels of HILP2 (except in the LK knockdown group), HILP5, and HILP8 increased, whereas those of HILP3, HILP4, and HILP6 decreased; HILP1, HILP2 (in the LK knockdown group), and HILP7 gene expression was not influenced after LK and LKR knockdown. Variations in mRNA expression levels in insulin-like peptide genes in the knockdown larvae suggest an essential role of these genes in survival in H. cunea. To our knowledge, the present study is the first comprehensive study of LK and LKR – from gene to behavior – in H. cunea.

show abstract

Section: Discussionmentioning

confidence: 99%

Functional Identification and Characterization of Leucokinin and Its Receptor in the Fall Webworm, Hyphantria cunea

Sun

Gao

et al. 2021

Front. Physiol.

View full text Add to dashboard Cite

show abstract

“…These cDNAs were subjected to quantitative reverse transcription polymerase chain reaction (qRT‐PCR) using the TB Green™ Premix Ex Taq™ (Takara Bio) in a 20 μL reaction system on a CFX Connect TM Real‐Time PCR System (Bio‐Rad, Hercules, CA, USA). GAPDH (accession number: KJ094948.1) and actin (accession number: KF022227.1) were used as internal reference genes 7 . The efficiency of qRT‐PCR primers was validated before gene expression analysis (Supporting Information Table S1).…”

Section: Methodsmentioning

confidence: 99%

“…GAPDH (accession number: KJ094948.1) and actin (accession number: KF022227.1) were used as internal reference genes. 7 The efficiency of qRT-PCR primers was validated before gene expression analysis (Supporting Information Table S1).…”

Section: Quantitative Reverse Transcription Polymerase Chain Reaction...mentioning

confidence: 99%

“…The oriental fruit moth (OFM), Grapholita molesta Busck (Lepidoptera: Tortricidae), is a major invasive pest on Rosaceae fruit trees and has become a growing problem in the fruit growing areas of Asia, Europe, America, and Australia. 7,8 The robust reproductive ability has been reported to be one of the major reasons for ineffective management in OFM. 9,10 Unlike OFM males can mate multiple times (up to four times) during their lifetime, the majority of OFM females only mate a single time.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Peroxiredoxin 1 transfer during mating protects eupyrene sperm against oxdative stress in Grapholita molesta

Cheng

Zhu

et al. 2023

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND Each Grapholita molesta female only copulates once during its lifetime and thus must maintain the viability of stored eupyrene sperm for male reproductive success. The male ejaculate comprises abundant accessory gland proteins produced by the male accessory gland (AG), and many of which are major effectors for sperm storage and maintenance. RESULTS Here, we reported that an antioxidant protein, peroxiredoxin 1 (GmolPrx1), secreted by the male AG, is essential for protecting eupyrene sperm from oxidative stress and maintaining their quality during storage in the female bursa copulatrix (BC). Our data showed that GmolPrx1 is highly expressed in the AG of sexually mature males. The GmolPrx1 protein is localized to the cytoplasm of AG cells and delivered to the female BC during mating. Knockdown of GmolPrx1 strongly decreased the fertility of mated females. Additionally, we evaluated oxidative status in the spermatophore of females and found that the content of hydrogen peroxide increased significantly after mating with GmolPrx1 knockdown males. Finally, the quality assessment of eupyrene sperm demonstrated that the plasma membrane integrity, acrosome integrity, and DNA integrity were all severely impaired in the spermatophore of females after mating with GmolPrx1 knockdown males, which may contribute to the fertility decline in males. CONCLUSION Our current data demonstrated that activities of eupyrene sperm stored in females can be significantly impaired by enhanced oxidative stress through knocking down of GmolPrx1 in males. Our finding thus may further lay new foundations for the control of G. molesta through suppressing their populations by manipulating male reproductive genes. © 2023 Society of Chemical Industry.

show abstract

“…Its cognate receptor sNPFR belongs to the rhodopsin superfamily of G protein-coupled receptors (GPCRs), which share a 7 α-helical transmembrane structure (Mertens et al, 2002). To date, sNPF and its receptor have been reported in many insects, such as Diptera (Nässel et al, 2008;Li et al, 2022), Orthoptera (Dillen et al, 2013(Dillen et al, , 2014, and Coleoptera (Marciniak et al, 2020;Liu et al, 2021a), Hemiptera (Peng et al, 2021;Amir et al, 2022), Hymenoptera (Lu & Pietrantonio, 2011;Bestea et al, 2022b), and Lepidoptera (Nagata et al, 2012;Ma et al, 2017;Cheng et al, 2021). These data suggest that sNPF family neuropeptide signals widely exist in insect species.…”

Section: Introductionmentioning

confidence: 99%

Effect of short neuropeptide F signaling on larval feeding in Mythimna separata

Yang

Chen

et al. 2023

Insect Science

View full text Add to dashboard Cite

Mythimna separata is a notorious phytophagous pest which poses serious threats to cereal crops owing to the gluttony of the larvae. Because short neuropeptide F (sNPF) and its receptor sNPFR are involved in a diversity of physiological functions, especially in functions related to feeding in insects, it is a molecular target for pest control. Herein, an sNPF and 2 sNPFRs were identified and cloned from M. separata. Bioinformatics analysis revealed that the sNPF and its receptors had a highly conserved RLRFamide C‐terminus and 7 transmembrane domains, respectively. The sNPF and its receptor genes were distributed across larval periods and tissues, but 2 receptors had distinct expression patterns. The starvation‐induced assay elucidated that sNPF and sNPFR expression levels were downregulated under food deprivation and recovered with subsequent re‐feeding. RNA interference knockdown of sNPF, sNPFR1, and sNPFR2 by injection of double‐stranded RNA into larvae not only suppressed food consumption and increased body size and weight, but also led to decrease of glycogen and total lipid contents, and increase of trehalose compared with double‐stranded green fluorescent protein injection. Furthermore, molecular docking was performed on the interaction mode between sNPFR protein and its ligand sNPF based on the 3‐dimensional models constructed by AlphaFold; the results indicated that both receptors were presumably activated by the mature peptide sNPF‐2. These results revealed that sNPF signaling played a considerably vital role in the feeding regulation of M. separata and represents a potential control target for this pest.

show abstract

Coordinated transcriptomics and peptidomics of central nervous system identify neuropeptides and their G protein-coupled receptors in the oriental fruit moth Grapholita molesta

Cited by 3 publications

References 84 publications

Functional Identification and Characterization of Leucokinin and Its Receptor in the Fall Webworm, Hyphantria cunea

Functional Identification and Characterization of Leucokinin and Its Receptor in the Fall Webworm, Hyphantria cunea

Peroxiredoxin 1 transfer during mating protects eupyrene sperm against oxdative stress in Grapholita molesta

Effect of short neuropeptide F signaling on larval feeding in Mythimna separata

Contact Info

Product

Resources

About