Coordinated transcriptional regulation of the carotenoid biosynthesis contributes to fruit lycopene content in high-lycopene tomato genotypes

Duduit, James R; Kosentka, Pawel Z; Miller, Morgan A; Blanco‐Ulate, Barbara; Lenucci, Marcello Salvatore; Panthee, Dilip R.; Perkins‐Veazie, Penelope; Liu, Wusheng

doi:10.1093/hr/uhac084

Cited by 6 publications

(2 citation statements)

References 70 publications

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“…Carotenoids have various health benefits, including antioxidant activity (Iannone et al., 1998), preventive effects on cardiovascular disease (Wang, Chung, et al., 2014), anti‐cancer effects (Kucuk et al., 2010), and anti‐aging effects (Kusmita et al., 2021). Lycopene is one of the carotenoids with relatively high antioxidant activity (Duduit et al., 2022). There are not many plants that can accumulate high levels of lycopene, which indicates its lycopene accumulation mechanism is extraordinary.…”

Section: Discussionmentioning

confidence: 99%

Lycopene ε‐cyclase mediated transition of α‐carotene and β‐carotene metabolic flow in carrot fleshy root

Wang

Zhang

et al. 2023

The Plant Journal

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SUMMARYThe accumulation of carotenoids, such as xanthophylls, lycopene, and carotenes, is responsible for the color of carrot (Daucus carota subsp. sativus) fleshy roots. The potential role of DcLCYE, encoding a lycopene ε‐cyclase associated with carrot root color, was investigated using cultivars with orange and red roots. The expression of DcLCYE in red carrot varieties was significantly lower than that in orange carrots at the mature stage. Furthermore, red carrots accumulated larger amounts of lycopene and lower levels of α‐carotene. Sequence comparison and prokaryotic expression analysis revealed that amino acid differences in red carrots did not affect the cyclization function of DcLCYE. Analysis of the catalytic activity of DcLCYE revealed that it mainly formed ε‐carotene, while a side activity on α‐carotene and γ‐carotene was also observed. Comparative analysis of the promoter region sequences indicated that differences in the promoter region may affect the transcription of DcLCYE. DcLCYE was overexpressed in the red carrot ‘Benhongjinshi’ under the control of the CaMV35S promoter. Lycopene in transgenic carrot roots was cyclized, resulting in the accumulation of higher levels of α‐carotene and xanthophylls, while the β‐carotene content was significantly decreased. The expression levels of other genes in the carotenoid pathway were simultaneously upregulated. Knockout of DcLCYE in the orange carrot ‘Kurodagosun’ by CRISPR/Cas9 technology resulted in a decrease in the α‐carotene and xanthophyll contents. The relative expression levels of DcPSY1, DcPSY2, and DcCHXE were sharply increased in DcLCYE knockout mutants. The results of this study provide insights into the function of DcLCYE in carrots, which could serve as a basis for creating colorful carrot germplasms.

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Section: Discussionmentioning

confidence: 99%

Lycopene ε‐cyclase mediated transition of α‐carotene and β‐carotene metabolic flow in carrot fleshy root

Wang

Zhang

et al. 2023

The Plant Journal

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“…qPCR was performed in a CFX96 Touch Real-Time PCR detection system (Bio-Rad Laboratories; Hercules, CA, USA) using the FastStart Universal SYBR Green Master (Roche Diagnostics Corporation; Indianapolis, IN, USA) as described in Zhao et al . [ 77 ] and Duduit et al [ 92 ]. The optimal annealing temperature, primer concentration, and appropriate cDNA concentrations were determined to get the lowest Cycle threshold (Ct) value as the optimal conditions prior to the qPCR.…”

Section: Methodsmentioning

confidence: 99%

Engineered Cleistogamy inCamelina sativafor bioconfinement

Huang

Gao

McAdams

et al. 2022

Horticulture Research

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Summary Camelina sativa is a self-pollinating and facultative outcrossing oilseed crop. Genetic engineering has been used to improve camelina yield potential for altered fatty acid composition, modified protein profiles, improved seed and oil yield, and enhanced drought resistance. The deployment of transgenic camelina in the field posits high risks related to the introgression of transgenes into non-transgenic camelina and wild relatives. Thus, effective bioconfinement strategies need to be developed to prevent pollen-mediated gene flow (PMGF) from transgenic camelina. In the present study, we overexpressed the cleistogamy (i.e. floral petal non-openness)-inducing PpJAZ1 gene from peach in transgenic camelina. Transgenic camelina overexpressing PpJAZ1 showed three levels of cleistogamy, affected pollen germination rates after anthesis but not during anthesis, and caused a minor silicle abortion only on the main branches. We also conducted field trials to examine the effects of the overexpressed PpJAZ1 on PMGF in the field, and found that the overexpressed PpJAZ1 dramatically inhibited PMGF from transgenic camelina to non-transgenic camelina under the field conditions. Thus, the engineered cleistogamy using the overexpressed PpJAZ1 is a highly effective bioconfinement strategy to limit PMGF from transgenic camelina, and could be used for bioconfinement in other dicot species.

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Edible flowers of India as alternate source of high quantity of lycopene

Majumder¹,

Mishra

2023

Vegetos

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Coordinated transcriptional regulation of the carotenoid biosynthesis contributes to fruit lycopene content in high-lycopene tomato genotypes

Cited by 6 publications

References 70 publications

Lycopene ε‐cyclase mediated transition of α‐carotene and β‐carotene metabolic flow in carrot fleshy root

Lycopene ε‐cyclase mediated transition of α‐carotene and β‐carotene metabolic flow in carrot fleshy root

Engineered Cleistogamy inCamelina sativafor bioconfinement

Edible flowers of India as alternate source of high quantity of lycopene

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