Coordinated transcriptional regulation of calmegin, a testis-specific molecular chaperon, by histone deacetylase and CpG methyltransferase

Kim, Dong Hoon; Shim, Joong Sup; Kwon, Ho Jeong

doi:10.1038/emm.2005.61

Cited by 8 publications

(6 citation statements)

References 17 publications

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“…ChIPs were performed as described previously (Cho et al , 2001; Kim et al , 2005). The PCR signals were quantified using a Phosphoimager (Fujix BAS 2040) and normalized to the input DNA and the intergenic control.…”

Section: Methodsmentioning

confidence: 99%

Histone chaperones regulate histone exchange during transcription

Kim

Seol

Han

et al. 2007

EMBO J

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Transcription by RNA polymerase II is accompanied by dynamic changes in chromatin, including the eviction/deposition of nucleosomes or the covalent modification of histone subunits. This study examined the role of the histone H3/H4 chaperones, Asf1 and HIR, in histone mobility during transcription, with particular focus on the histone exchange pathway, using a dual histone expression system. The results showed that the exchange of H3/H4 normally occurs during transcription by the histone chaperones. Both Asf1 and HIR are important for histone deposition but have a different effect on histone exchange. While Asf1 mediated incorporation of external H3/H4 and renewal of pre-existing histones, HIR opposed it. The balance of two opposing activities might be an important mechanism for determining current chromatin states.

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Section: Methodsmentioning

confidence: 99%

Histone chaperones regulate histone exchange during transcription

Kim

Seol

Han

et al. 2007

EMBO J

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“…29 CLGN has been reported to act as a chaperone, which is involved in the histone-related pathway of cell division control. 30 Further molecular analyses based on the up-or down-regulated genes will be reported in a later separated paper. …”

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confidence: 99%

Synthesis of 8-Hydroxyquinoline Derivatives as Novel Antitumor Agents

Chan

Chui

Chan

et al. 2012

ACS Med. Chem. Lett.

103

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This letter describes the preparation of quinoline derivatives and their cytotoxic potentials toward human carcinoma cell lines. Among the selected compounds, 8-hydroxy-2-quinolinecarbaldehyde (3) showed the best in vitro cytotoxicity against the human cancer cell lines, including MDA231, T-47D, Hs578t, SaoS2, K562, SKHep1 (with a MTS 50 range of 12.5−25 μg/mL) and Hep3B (with a MTS 50 range of 6.25±0.034 μg/mL). The in vivo antitumor activity of compound 3 on subcutenaous Hep3B hepatocellular carcinoma xenograft in athymic nude mice was then studied. The results showed that the dose of 10 mg/kg/day of compound 3 with intraperitoneal injection for 9 days totally abolished the growth of the xenograft tumor of Hep3B with no histological damage on vital organs as compared with the control. The experimental results suggested that compound 3 has a good potential as an antitumor agent.

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“…Using 10 µg of protein, each reaction was performed as manufacturer's instruction in a 96 well-plate. After the reaction, HDAC activity was measured with a GENios microplate fluorometer having the Magellan software system (TECAN, Austria) with excitation at 360 nm and emission at 465 nm (Kim et al, 2005).…”

Section: Cell Culture and Proliferation Assaymentioning

confidence: 99%

Psammaplin A is a natural prodrug that inhibits class I histone deacetylase

Kim

Shin²,

Kwon³

2007

Exp Mol Med

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Histone deacetylase (HDAC) has been highlighted as one of key players in tumorigenesis and angiogenesis. Recently, several derivatives of psammaplin (Psams) from a marine sponge have been known to inhibit the HDAC activity, but the molecular mechanism for the inhibition has not fully understood. Here, we explored the mode of action of Psams for the inhibition of HDAC activity in the molecular and cellular level. Among the derivatives, psammaplin A (Psam A) showed the potent inhibitory activity in enzyme assay and anti-proliferation assay with IC 50 value of 0.003 and 1 µM, respectively. Psam A selectively induced hyperacetylation of histones in the cells, resulting in the upregulation of gelsolin, a well-known HDAC target gene, in a transcriptional level. In addition, reduced Psam A showed a stronger inhibitory activity than that of non-reduced one. Notably, glutathione-depleted cells were not sensitive to Psam A, implying that cellular reduction of the compound is responsible for the HDAC inhibition of Psam A after uptake into the cells. Together, these data demonstrate that Psam A could exhibit its activity under the reduced condition in the cells and be a new natural prodrug targeting HDAC.

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Coordinated transcriptional regulation of calmegin, a testis-specific molecular chaperon, by histone deacetylase and CpG methyltransferase

Cited by 8 publications

References 17 publications

Histone chaperones regulate histone exchange during transcription

Histone chaperones regulate histone exchange during transcription

Synthesis of 8-Hydroxyquinoline Derivatives as Novel Antitumor Agents

Psammaplin A is a natural prodrug that inhibits class I histone deacetylase

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