Coordinated RNA-Seq and peptidomics identify neuropeptides and G-protein coupled receptors (GPCRs) in the large pine weevil Hylobius abietis, a major forestry pest

Pandit, Aniruddha A.; Ragionieri, Lapo; Marley, Richard; Yeoh, Joseph G.C.; Inward, Daegan J. G.; Davies, Shireen-A.; Predel, Reinhard; Dow, Julian A. T.

doi:10.1016/j.ibmb.2018.08.003

Cited by 17 publications

(27 citation statements)

References 76 publications

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“…neuropeptides predicted from the short splice variant of npf gene have been identified in neuropeptidomic of these insects (Nässel and Wegener, 2011;Pandit et al, 2018), whether long splice variant of npf gene encodes different mature neuropeptide remains unclear.…”

Section: Identification Of Various Neuropeptidesmentioning

confidence: 99%

“…The orcokinin B precursor can be processed into several similar isopeptides, and the number of peptides varies from species to species. The number of isoorcokinin-B is predicted 22 for the red palm beetle, more than 15 in H. abietis (Pandit et al, 2018), 10 in T. castaneum , and only one in D. melanogaster (Veenstra and Ida, 2014). The consensus for Orcokinin B isopeptides is X(I, L, V)DXXGGG in N-terminal based on sequence alignments (Supplementary Figure S3).…”

Section: Identification Of Various Neuropeptidesmentioning

confidence: 99%

“…In the red palm weevil, a transcript encoding calcitonin-B was identified. In both T. castaneum and H. abietis, two genes coding for calcitonin-B produce four and six isopeptides, respectively (Veenstra, 2014;Pandit et al, 2018). The red palm weevil transcript encodes seven mature isopeptides ( Supplementary Table S3).…”

Section: Identification Of Various Neuropeptidesmentioning

confidence: 99%

“…A pyrokinin and Capa precursor in hexapods can result in three types of neuropeptides: periviscerokinins (PVKs), pyrokinins (PKs), and tryptoPKs, and each one activates a specific receptor (Terhzaz et al, 2012;Pandit et al, 2018). The number and combination of these three peptides differ in different species based on different ways of gene duplication and subsequent diversification (Derst et al, 2016).…”

Section: Identification Of Various Neuropeptidesmentioning

confidence: 99%

“…Very limited information is available on neuropeptides and their functions on the red palm weevil. Base on genomics, transcriptomics, and peptidomics, numerous neuropeptides and receptors have been identified from Coleopterans (Weaver and Audsley, 2008;Cunningham et al, 2017;Pandit et al, 2018;Veenstra, 2019). The types and numbers of neuropeptides and receptors in Coleopterans are quite different from those from other insect species.…”

Section: Introductionmentioning

confidence: 99%

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Neuropeptides and G-Protein Coupled Receptors (GPCRs) in the Red Palm Weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae)

et al. 2020

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The red palm weevil Rhynchophorus ferrugineus is a devastating, invasive pest that causes serious damages to palm trees, and its invasiveness depends on its strong ability of physiological and behavioral adaptability. Neuropeptides and their receptors regulate physiology and behavior of insects, but these protein partners have not been identified from many insects. Here, we systematically identified neuropeptide precursors and the corresponding receptors in the red palm weevil, and analyzed their tissue expression patterns under control conditions and after pathogen infection. A total of 43 putative neuropeptide precursors were identified, including an extra myosuppressin peptide was identified with amino acid substitutions at two conserved sites. Forty-four putative neuropeptide receptors belonging to three classes were also identified, in which neuropeptide F receptors and insulin receptors were expanded compared to those in other insects. Based on qRT-PCR analyses, genes coding for several neuropeptide precursors and receptors were highly expressed in tissues other than the nervous system, suggesting that these neuropeptides and receptors play other roles in addition to neuro-reception. Some of the neuropeptides and receptors, like the tachykininrelated peptide and receptor, were significantly induced by pathogen infection, especially sensitive to Bacillus thuringiensis and Metarhizium anisopliae. Systemic identification and initial characterization of neuropeptides and their receptors in the red palm weevil provide a framework for further studies to reveal the functions of these ligand-and receptor-couples in regulating physiology, behavior, and immunity in this important insect pest species.

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Section: Identification Of Various Neuropeptidesmentioning

confidence: 99%

Section: Identification Of Various Neuropeptidesmentioning

confidence: 99%

Section: Identification Of Various Neuropeptidesmentioning

confidence: 99%

Section: Identification Of Various Neuropeptidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Neuropeptides and G-Protein Coupled Receptors (GPCRs) in the Red Palm Weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae)

et al. 2020

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Structural diversity of adipokinetic hormones in the hyperdiverse coleopteran Cucujiformia

GÄde

Šimek

Marco

2019

Arch Insect Biochem Physiol

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Seventeen species of the coleopteran series Cucujiformia are investigated for the presence and sequence of putative adipokinetic hormones (AKHs). Cucujiformia includes species from the major superfamilies, that is, Chrysomeloidea, Curculionoidea, Cucujoidea, and Tenebrionoidea. The clade Phytophaga in which the Chrysomeloidea and Curculionoidea reside, harbor very detrimental species for agriculture and forestry. Thus, this study aims not only to demonstrate the structural biodiversity of AKHs in these beetle species and possible evolutionary trends but also to determine whether the AKHs from harmful pest species can be used as lead substances for a future putative insecticide that is harmless to beneficial insects. Sequence analysis of AKHs is achieved by liquid chromatography coupled to mass spectrometry. Most of the investigated species contain AKH octapeptides in their corpora cardiaca, although previously published work also found a few decapeptides, which we comment on. The signature and sole AKH in cerambycidae Chrysomeloidea and Curculionoidea is Peram-CAH-I (pEVNFSPNW amide), which is also found in the majority of chrysomelidae Chrysomeloidea and in the one investigated species of Cucujoidea albeit in a few cases associated with a second AKH which can be either Peram-CAH-II (pELTFTPNW amide), Emppe-AKH (pEVNFTPNW amide), or Micvi-CC (pEINFTPNW amide). The most often encountered AKH in Tenebrionoidea, family Meloidae as well as family Tenebrionidae, is Tenmo-HrTH (pELNFSPNW amide) followed by Pyrap-AKH (pELNFTPNW amide) and a Tenmo-HrTH extended decapeptide (in Meloidae). Finally, we examine AKH sequences from 43 species of cucujiform beetles, including the superfamily Coccinelloidea for a possible lead compound for producing a cucujiform-specific pesticide.

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Short neuropeptide F signaling regulates functioning of male reproductive system in Tenebrio molitor beetle

et al. 2020

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Neuropeptides of short neuropeptides F family (sNPF) have been identified in various arthropods. They are pleiotropic neuromolecules which so far have been mainly associated with regulation of feeding and metabolism, as well as growth and development, locomotion, circadian rhythm or learning and memory. Here, we describe the effects of Tenebrionid sNPF peptide (SGRSPSLRLRFa) on various aspects of the male reproductive physiology in the Tenebrio molitor beetle. We identified in silico the putative sNPF receptor Tenmo-sNPFR. Based on RT-PCR technique, it was shown that the receptor might be present in the male reproductive tissues of this beetle. The analysis of receptor amino acid sequence showed that it is similar to other beetle sNPFRs, as well as other insect species, and belongs rhodopsin-like G-protein-coupled receptors (GPCRs). Injections of Trica-sNPF and its shorter form Trica-sNPF (4-11) caused differentiated effects in T. molitor male reproductive tissues. After 24 h post injections, the peptides decreased the concentration of the soluble protein fraction in testes of 4-and 8-day-old beetles as well as the dry mass of these organs but only in 8-day-old individuals. The same effects were shown with regard to accessory glands. Both peptides decrease the concentration of the soluble protein fraction but do not affect the dry mass of this organ. Furthermore, injections of Trica-sNPF at the 10-7 M concentration decrease the total sperm number in the reproductive system. Surprisingly, the same concentration of the shorter form, Trica-sNPF (4-11) increased the sperm number. It was also shown that both peptides in different manner influence contractions of ejaculatory duct. The data presented in this article give new evidence that sNPFs are involved in the regulation of reproductive events in beetles, which might be the part of a larger neuropeptide network combining feeding, growth and development with the physiology of reproduction.

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Coordinated RNA-Seq and peptidomics identify neuropeptides and G-protein coupled receptors (GPCRs) in the large pine weevil Hylobius abietis, a major forestry pest

Cited by 17 publications

References 76 publications

Neuropeptides and G-Protein Coupled Receptors (GPCRs) in the Red Palm Weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae)

Neuropeptides and G-Protein Coupled Receptors (GPCRs) in the Red Palm Weevil Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae)

Structural diversity of adipokinetic hormones in the hyperdiverse coleopteran Cucujiformia

Short neuropeptide F signaling regulates functioning of male reproductive system in Tenebrio molitor beetle

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